Coherent Quenching of a Fluorophore for the Design of a Highly Sensitive In‐Stem Molecular Beacon

Abstract

Excitonische Wechselwirkung wurde beim Design eines hochempfindlichen „molekularen Beacons“ genutzt, der in seiner Stammregion D‐Threoninole mit Fluorophor und Fluoreszenzlöscher als Pseudobasenpaare trägt (ISMB; siehe Schema: optimierte Kombination mit Cy3 und modifiziertem Methylrot). Durch Minimierung der Differenz zwischen λmax von Fluorophor und Fluoreszenzlöscher wurde die Löscheffizienz maximiert.

Rapid Electron Transfer of Stacked Heterodimers of Perylene Diimide Derivatives in a DNA Duplex

“…Functional nucleic acids with the ability to generate an emission based on a binding event in the presence of target molecules have been designed and characterized . Molecular beacons (MBs) are DNA hairpin structures with a pair of fluorophore and quencher molecules, which are designed to convert recognition events into a fluorescence signal through conformational changes in the DNA hairpin from the closed to open form, and are utilized in DNA/RNA targeting applications, intracellular mRNA imaging, real‐time PCR, and fluorescence biosensors . To improve the sensitivity and specificity of the MBs to target molecules, many approaches have been applied .…”

Visual Scanometric Detection of DNA through Silver Enhancement Regulated by Gold‐Nanoparticle Aggregation with a Molecular Beacon as the Trigger

“…Recently, MBs have received considerable interest because of the inherent signaling mechanism by energy transfer and the high selectivity in the detection of DNA sequences and proteins. [22][23][24][25][26][27] Different from regular MBs that contain stems based on the Watson-Crick system, MBs based on a non-Watson-Crick system show greater selectivity, control, and resistance toward nuclease digestion than Abstract: A convenient and label-free scanometric approach for DNA assay was designed by integrating a metalion-mediated conformational molecular beacon (MB) and silver-signal amplification regulated by gold-nanoparticle (AuNP) aggregation. The strategy was based on displacing the interaction between the target DNA sequence and a competitor Hg 2 + ion with a link DNA sequence.…”

In‐Stem‐Labeled Molecular Beacons for Distinct Fluorescent Color Readout

“…The most important developments in this area have been quencher-free MBs, [3] low-noise stemless PNA MBs, [4] wavelength-shifting MBs, [5,6] and MBs based on excimer fluorescence color readout. [7][8][9][10][11] Recently, we reported that two thiazole orange (TO) chromophores as artificial nucleobases in DNA form a hydrophobically interacting interstrand dimer which results in a distinct change in fluorescence color upon DNA hybridization. [7] Herein we present an advanced design of in-stemlabeled, wavelength-shifting MBs based on the combination of TO and thiazole red (TR) as an interstrand chromophore pair for energy transfer (ET).…”

“…In comparison with conventional MBs like DNA6 and other recently published MBs [3][4][5][6][7][8][9][10][11] our approach has two major advantages: 1) The fluorescence readout allows a clear and distinct discrimination simply by the emission color (140 nm shift). 2) As a result of the well-separated emission bands, the remarkably high contrast f between duplex and hairpin form enhances the signal-to-noise ratio.…”