Coherent imaging of biological samples with femtosecond pulses at the free-electron laser FLASH

Mancuso, Adrian P.; Gorniak, Thomas; Staier, Florian; Yefanov, Oleksandr; Barth, R.; Christophis, Christof; Reime, B.; Gulden, J.; Singer, Andrej; Pettit, Michala E.; Nisius, Thomas; Wilhein, T.; Gutt, Christian; Grübel, G.; Guerassimova, N.; Treusch, R.; Feldhaus, J.; Eisebitt, Stefan; Weckert, E.; Grunze, M.; Rosenhahn, Axel; Vartanyants, Ivan A.

doi:10.1088/1367-2630/12/3/035003

Cited by 79 publications

(58 citation statements)

References 40 publications

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“…For the discrete Fourier transform of a cyclic convolution of two sequences f N and g N one obtains in analogy to the continuous case 91) for F N and G N denoting the discrete Fourier transforms of f N and g N . Discrete correlation.…”

Section: The Discrete Fourier Transform 19mentioning

confidence: 99%

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A study on new approaches in coherent x-ray microscopy of biological specimens

Giewekemeyer¹

2011

Göttingen Series in X-Ray Physics

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Section: The Discrete Fourier Transform 19mentioning

confidence: 99%

“…beamline UE52-SGM of the Berlin electron storage ring Bessy II, a dedicated experimental vacuum chamber [91] was used that has been developed by the group of Dr. Axel Rosenhahn at the University of Heidelberg. The incident beam was focused by mirrors and/or confined by slits to a size of about 17.4(hrz.)…”

mentioning

confidence: 99%

A study on new approaches in coherent x-ray microscopy of biological specimens

Giewekemeyer¹

2011

Göttingen Series in X-Ray Physics

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“…Besides the tight gap between the two bacteria no inner structure can be resolved in the reconstructed hologram. However, a reconstruction is possible, which was not the case with previous CXDI data in the 3rd harmonic [20]. In the cited CXDI study, insufficient coherence was indicated as the most likely cause for the problems during the reconstruction.…”

Section: 4mentioning

confidence: 89%

“…Even though progress in solving the phase problem has been made in the last years [17][18][19], it remains challenging. At FLASH, recent CXDI data in the 3rd harmonic at a photon energy of 462 eV was recorded, but the phase retrieval was hampered due to the limited coherence length in this case [20,21]. Contrary to CXDI, holography [22] is a coherent projection microscopy technique.…”

Section: Introductionmentioning

confidence: 99%

X-ray holographic microscopy with zone plates applied to biological samples in the water window using 3rd harmonic radiation from the free-electron laser FLASH

Gorniak¹,

Heine²,

Mancuso³

et al. 2011

Opt. Express

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Abstract:The imaging of hydrated biological samples -especially in the energy window of 284-540 eV, where water does not obscure the signal of soft organic matter and biologically relevant elements -is of tremendous interest for life sciences. Free-electron lasers can provide highly intense and coherent pulses, which allow single pulse imaging to overcome resolution limits set by radiation damage. One current challenge is to match both the desired energy and the intensity of the light source. We present the first images of dehydrated biological material acquired with 3rd harmonic radiation from FLASH by digital in-line zone plate holography as one step towards the vision of imaging hydrated biological material with photons in the water window. We also demonstrate the first application of ultrathin molecular sheets as suitable substrates for future free-electron laser experiments with biological samples in the form of a rat fibroblast cell and marine biofouling bacteria Cobetia marina. ©2011 Optical Society of America References and links1. C. A. Brau, "Free-electron lasers," Science 239(4844), 1115-1121 (1988). 2. J. Feldhaus, J. Arthur, and J. Hastings, "X-ray free-electron lasers," J. Phys. B: At. Mol. Opt. Phys. 38(9), S799-S819 (2005

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“…Nowadays, microscopy with a resolution down to the scale of proteins 5,6 is feasible at large-scale facilities such as synchrotrons and more recently at x-ray free-electron lasers. [7][8][9] However, for the routine operation in a clinical environment drawing on these large-scale facilities is not an option.…”

Section: Introductionmentioning

confidence: 99%

Cancer cell classification with coherent diffraction imaging using an extreme ultraviolet radiation source

et al. 2014

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Abstract. In cancer treatment, it is highly desirable to classify single cancer cells in real time. The standard method is polymerase chain reaction requiring a substantial amount of resources and time. Here, we present an innovative approach for rapidly classifying different cell types: we measure the diffraction pattern of a single cell illuminated with coherent extreme ultraviolet (XUV) laser-generated radiation. These patterns allow distinguishing different breast cancer cell types in a subsequent step. Moreover, the morphology of the object can be retrieved from the diffraction pattern with submicron resolution. In a proof-of-principle experiment, we prepared single MCF7 and SKBR3 breast cancer cells on gold-coated silica slides. The output of a laser-driven XUV light source is focused onto a single unstained and unlabeled cancer cell. With the resulting diffraction pattern, we could clearly identify the different cell types. With an improved setup, it will not only be feasible to classify circulating tumor cells with a high throughput, but also to identify smaller objects such as bacteria or even viruses.

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Coherent imaging of biological samples with femtosecond pulses at the free-electron laser FLASH

Cited by 79 publications

References 40 publications

A study on new approaches in coherent x-ray microscopy of biological specimens

A study on new approaches in coherent x-ray microscopy of biological specimens

X-ray holographic microscopy with zone plates applied to biological samples in the water window using 3rd harmonic radiation from the free-electron laser FLASH

Cancer cell classification with coherent diffraction imaging using an extreme ultraviolet radiation source

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