Coherence-controlled holographic microscope

Kolman, Pavel; Chmelík, Radim

doi:10.1364/oe.18.021990

Cited by 70 publications

(50 citation statements)

References 33 publications

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“…The cells were imaged with a coherence-controlled holographic microscope (CCHM). 24,25 The imaging in CCHM is based on the interference of the object and the reference light beams, which enables it to detect the phase delay induced by the specimen (SP). It has been demonstrated in several publications that the measured phase in the image corresponds to the dry mass density distribution within the cell.…”

Section: Introductionmentioning

confidence: 99%

Automated classification of cell morphology by coherence-controlled holographic microscopy

et al. 2017

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Abstract. In the last few years, classification of cells by machine learning has become frequently used in biology. However, most of the approaches are based on morphometric (MO) features, which are not quantitative in terms of cell mass. This may result in poor classification accuracy. Here, we study the potential contribution of coherence-controlled holographic microscopy enabling quantitative phase imaging for the classification of cell morphologies. We compare our approach with the commonly used method based on MO features. We tested both classification approaches in an experiment with nutritionally deprived cancer tissue cells, while employing several supervised machine learning algorithms. Most of the classifiers provided higher performance when quantitative phase features were employed. Based on the results, it can be concluded that the quantitative phase features played an important role in improving the performance of the classification. The methodology could be valuable help in refining the monitoring of live cells in an automated fashion. We believe that coherencecontrolled holographic microscopy, as a tool for quantitative phase imaging, offers all preconditions for the accurate automated analysis of live cell behavior while enabling noninvasive label-free imaging with sufficient contrast and high-spatiotemporal phase sensitivity.

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Section: Introductionmentioning

confidence: 99%

Automated classification of cell morphology by coherence-controlled holographic microscopy

et al. 2017

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“…6,15 The cell is a convenient thin and transparent specimen for this method. Nonaqueous components of the cell have similar specific refractive increments 16 higher than water.…”

Section: Coherence-controlled Holographic Microscopementioning

confidence: 99%

Coherence-controlled holographic microscopy enabled recognition of necrosis as the mechanism of cancer cells death after exposure to cytopathic turbid emulsion

et al. 2015

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“…[1][2][3][4][5][6][7] Although it is easier to obtain interference with a highly coherent source, using such a source in interferometric and holographic imaging in general, and in IPM in particular, significantly reduces the image quality due to parasitic interferences and coherent noise. [7][8][9][10][11][12] To overcome this problem, low-coherence light sources are employed. However, to obtain interference using these sources, meticulous alignment between the optical paths of the two beams is required.…”

Section: Introductionmentioning

confidence: 99%

“…9 The practical implication of this limitation is that large samples cannot be simultaneously recorded by off-axis interferometry on the entire camera sensor using low-coherence sources. Diffractive gratings can solve this problem by tilting the field of the beams to be in plane, [9][10][11][12][13] with the cost of possible aliasing and image modulation.…”

Section: Introductionmentioning

confidence: 99%

Doubling the field of view in off-axis low-coherence interferometric imaging

2014

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We present a new interferometric and holographic approach, named interferometry with doubled imaging area (IDIA), with which it is possible to double the camera field of view while performing off-axis interferometric imaging, without changing the imaging parameters, such as the magnification and the resolution. This technique enables quantitative amplitude and phase imaging of wider samples without reducing the acquisition frame rate due to scanning. The method is implemented using a compact interferometric module that connects to a regular digital camera, and is useful in a wide range of applications in which neither the field of view nor the acquisition rate can be compromised. Specifically, the IDIA principle allows doubling the off-axis interferometric field of view, which might be narrower than the camera field of view due to low-coherence illumination. We demonstrate the proposed technique for scan-free quantitative optical thickness imaging of microscopic biological samples, including live neurons and a human sperm cell in rapid motion under high magnification. In addition, we used the IDIA principle to perform non-destructive profilometry during a rapid lithography process of transparent structures.

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Coherence-controlled holographic microscope

Cited by 70 publications

References 33 publications

Automated classification of cell morphology by coherence-controlled holographic microscopy

Automated classification of cell morphology by coherence-controlled holographic microscopy

Coherence-controlled holographic microscopy enabled recognition of necrosis as the mechanism of cancer cells death after exposure to cytopathic turbid emulsion

Doubling the field of view in off-axis low-coherence interferometric imaging

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