Cognate interactions between helper T cells and B cells

Noelle, Randolph J.; Snow, E Charles

doi:10.1016/0167-5699(90)90142-v

Cited by 126 publications

(61 citation statements)

References 66 publications

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“…Indeed, although proteins need to encompass a processing step within an antigenpresenting cell, peptides can be recognized directly by the primed lymphocyte [17], After stimulation by peptides, cells from some P.falciparumprimed donors produced antibodies binding to the stimulating peptide but also to the other studied peptides. This observation suggests either the presence of crossed reactivity of the antibodies or some degree of by-standing cellular activation [18], that may be explained by the efficient triggering by peptides of primed T lymphocytes, Serologic studies demonstrated that antibodies to non-repeated sequences of Pfl55/RESA do not cross-react with repeats [10], Nevertheless, elucidation of the precise specificity of the produced antibodies would require further competition experiments.…”

Section: Discussionmentioning

confidence: 99%

Quantification of antibody-secreting lymphocytes that react with Pf155/RESA fromPlasmodium falciparum: an ELISPOT assay for field studies

Fiévet

Chougnet

Dubois

et al. 1993

Clinical and Experimental Immunology

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SUMMARYWe have adapted the enzyme-linked immunospot assay (ELISPOT) to enumerate the cells from Plasmodium falciparum-primed donors that produce IgG in vitro in response to malaria antigens. In vitro activation of cell cultures with two synthetic peptides (EENVEHDA)4, and (LGRSGGDIIKMQTL) corresponding to immunodominant T cell epitopes of the ring-infected erythrocyte surface antigen (Pf 155/RESA) gave specific antibody-secreting cells (ASC) in five and six of the 15 P, falciparum-primed donors from Cameroon. Antibodies produced after a stimulation by synthetic peptides reacted also with total parasite proteins. However, crude P, falciparum antigen did not trigger a higher number of cells than did synthetic peptides. The absence of significant relation between the presence of sera antibodies and in vitro ASC against the same peptide suggests that the kinetics of circulating primed lymphocytes and antibodies are different. We evaluated 004-0-29% of peripheral blood B cells to be the frequency of memory cells specific to a single Pfl 55/RESA epitope in these donors. This study suggests that the ELISPOT assay should permit the analysis of B cell responses to malarial antigens at the single-cell level and its applicability to epidemiological field studies. This assay should be well suited to the identification of T helper epitopes capable of inducing the production of antibodies by human B cells, and will constitute an important tool for the selection of immunogens to be included in a subunit vaccine.

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Section: Discussionmentioning

confidence: 99%

Quantification of antibody-secreting lymphocytes that react with Pf155/RESA fromPlasmodium falciparum: an ELISPOT assay for field studies

Fiévet

Chougnet

Dubois

et al. 1993

Clinical and Experimental Immunology

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“…Physical contact between T celis and antigen-presenting B cells is particularly important for B cell differentiation into immunoglobulin-producing cells [41]. This process would be important in the periodontitis lesion as well.…”

Section: Discussionmentioning

confidence: 99%

Immunohistological analysis of T cell functional subsets in chronic inflammatory periodontal disease

Yamazaki

Nakajima

Hara

1995

Clinical and Experimental Immunology

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SUMMARY IL‐2, interferon‐gamma (IFN‐γ), IL‐4 and IL‐6 producing T cells in periodontitis and gingivitis‐affected human tissues were investigated by immunohistochemistry to clarify the relationship between T cell functional subsets and disease entity. Using alkaline‐phosphatase anti‐alkaline‐phosphatase technique, the relative proportions of each cytokine‐producing T cell were calculated in the crevicular 1/3, middle 1/3 and oral 1/3 areas selected in the connective tissue of sections. CD19:CD3 and CD4:CD8 ratios were determined on the serial sections. Compared with gingivitis tissues, the proportion of cytokine‐producing cells in periodontitis‐affected samples was higher overall in the crevicular 1/3 (P < 0·02). The middle 1/3 exhibited a higher percentage of cytokine‐producing cells, except for IL‐6‐producing cells. Frequencies of cytokine‐producing cells in the oral 1/3 did not differ. IL‐4 was the prominent cytokine in periodontitis‐affected tissues, with the highest proportion detected in the crevicular 1/3. The CD19:CD3 ratio was higher in periodontitis tissues irrespective of the location, indicating a B cell dominance in periodontitis lesions. Furthermore, a significant positive correlation between the proportion of IL‐4‐producing cells and the CD19:CD3 ratio was noted. The CD4:CD8 ratio consistently exceeded 2·0 in both periodontitis and gingivitis. These results suggest that immunoregulation of both periodontitis and gingivitis are T cell‐dependent, but in periodontitis type 2 helper T cells predominate and thereby control B cell activation.

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“…Promiscuous S phase entry induced by D1/T286A is countered by apoptosis in young transgenic mice A majority of B cells are quiescent until exposed to antigen (Noelle and Snow, 1990). Given that cyclin D1 overexpression results in reduced growth factor requirements and premature S phase entry in cultured cells (Quelle et al, 1993), we considered the possibility that D1/T286A might trigger the promiscuous cell cycle entry of B lymphocytes.…”

Section: D1/t286a Associates With Cdk4 In Transgenic Lymphocytesmentioning

confidence: 99%

Expression of constitutively nuclear cyclin D1 in murine lymphocytes induces B-cell lymphoma

et al. 2005

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Mantle cell lymphoma (MCL) is a B-cell lymphoma characterized by overexpression of cyclin D1 due to the t(11;14) chromosomal translocation. While expression of cyclin D1 correlates with MCL development, expression of wild-type (WT) cyclin D1 transgene in murine lymphocytes is unable to drive B-cell lymphoma. As cyclin D1 mutants that are refractory to nuclear export display heighten oncogenicity in vitro compared with WT D1, we generated mice expressing FLAG-D1/T286A, a constitutively nuclear mutant, under the control of the immunoglobulin enhancer, El. D1/T286A transgenic mice universally develop a mature B-cell lymphoma. Expression of D1/T286A in B lymphocytes results in S phase entry in resting lymphocytes and increased apoptosis in spleens of young premalignant mice. Lymphoma onset correlates with perturbations in p53/ MDM2/p19 Arf expression and with BcL-2 overexpression suggesting that alterations in one or both of these pathways may contribute to lymphoma development. Our results describe a cyclin D1-driven model of B-cell lymphomagenesis and provide evidence that nuclearretention of cyclin D1 is oncogenic in vivo.

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Cognate interactions between helper T cells and B cells

Cited by 126 publications

References 66 publications

Quantification of antibody-secreting lymphocytes that react with Pf155/RESA fromPlasmodium falciparum: an ELISPOT assay for field studies

Quantification of antibody-secreting lymphocytes that react with Pf155/RESA fromPlasmodium falciparum: an ELISPOT assay for field studies

Immunohistological analysis of T cell functional subsets in chronic inflammatory periodontal disease

Expression of constitutively nuclear cyclin D1 in murine lymphocytes induces B-cell lymphoma

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