Coffee consumption induces GSTP in plasma and protects lymphocytes against (±)-anti-benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide induced DNA-damage: Results of controlled human intervention trials

Steinkellner, Hans; Hoelzl, Christine; Uhl, Maria; Cavin, Christophe; Haidinger, Gerald; Gsur, Andrea; Schmid, Rainer; Kundi, Michael; Bichler, Julia; Knasmüller, Siegfried

doi:10.1016/j.mrfmmm.2005.04.016

Cited by 66 publications

(48 citation statements)

References 66 publications

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“…Before that there are no significant effects on DNA damage, but markedly reduced hGSTP1 levels. There was also a significant correlation (p = 0.005) between hGSTP1 protein levels and oxidative DNA damage, which agrees well with other studies showing that increased hGSTP1 values were accompanied by a reduction of DNA damage in human leucocytes [40]. The immediate decrease of hGSTP1 protein expression is probably not due to the direct toxicity of the fruit juice polyphenols, since our in vitro studies do not indicate a particular vulnerability of the peripheral leucocytes after polyphenol incubation.…”

Section: Discussionsupporting

confidence: 91%

Intervention with polyphenol‐rich fruit juices results in an elevation of glutathione S‐transferase P1 (hGSTP1) protein expression in human leucocytes of healthy volunteers

Hofmann

Liegibel

Winterhalter

et al. 2006

Molecular Nutrition Food Res

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Polyphenols are probably antigenotoxic on account of their antioxidant activities and might alter phase I and II enzymes in a way that results in chemoprotection. We investigated the hypothesis that polyphenols enhance expression of glutathione S-transferases (GSTs), which increases carcinogen detoxification and thereby provides protection against oxidative stress. HGSTP1 protein expression and GST polymorphisms were determined in leucocytes obtained during an intervention study with healthy subjects consuming two fruit juices in an 8 wk trial (polyphenol-free run in phase, juice intervention phase, washout phase, second juice intervention phase, each treatment regime lasted for 2 wk). The study had originally shown that juice intervention significantly reduced oxidative DNA damage in leucocytes at week 8 (Bub, A., Watzl, B., Blockhaus, M., Briviba, K. et al., J. Nutr. Biochem. 2003, 14, 90-98). We reanalysed the levels of DNA damage based on GST genotypes. We also treated leucocytes in vitro with mixtures of polyphenols and determined cytotoxicity and expression of 96 genes related to drug metabolism. Key results with leucocytes of the intervention study were that the initial content of hGSTP1 protein was first suppressed at weeks 4 and 6. At week 8, however, hGSTP1 protein expression was significantly increased. HGSTP1 protein levels and DNA damage were inversely correlated (p = 0.005), but there was no difference for cells obtained from subjects with hGSTM1*1 and hGSTM1*0 genotypes, nor was there any difference between cells from subjects consuming the two different juices. The treatment of leucocytes with polyphenol mixtures in vitro did not result in modulated GST gene expression or total GST activity, but in an up-regulation of other biotransformation enzymes (e. g., members of the cytochrome P450 and the sulphotransferase family). In conclusion, in vitro treatment of leucocytes led to a modulated mRNA expression of selected genes, not directly related to oxidative defence systems. In vivo, however, we observed a delayed enhancement of hGSTP1, which could be associated with an initial repression of oxidative DNA damage in leucocytes from human subjects, consuming juices with high levels of polyphenols.

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Section: Discussionsupporting

confidence: 91%

Intervention with polyphenol‐rich fruit juices results in an elevation of glutathione S‐transferase P1 (hGSTP1) protein expression in human leucocytes of healthy volunteers

Hofmann

Liegibel

Winterhalter

et al. 2006

Molecular Nutrition Food Res

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“…1B). High ROS levels have been already reported to induce the Nrf2/ ARE pathway [11]. Cell cytotoxicity, inhibiting regular cellular response to oxidative stress, might decrease Nrf2-nuclear translocation, thereby explaining the here detected effects.…”

supporting

confidence: 66%

Coffees rich in chlorogenic acid or N‐methylpyridinium induce chemopreventive phase II‐enzymes via the Nrf2/ARE pathway in vitro and in vivo

Boettler

Volz

Pahlke

et al. 2011

Molecular Nutrition Food Res

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Recently, the coffee constituents 5-O-caffeoylquinic acid (CGA) and N-methylpyridinium (NMP) were identified as inducers of the Nrf2/antioxidant-response element (ARE) detoxifying pathway under cell-culture condition. To study the impact of CGA and NMP on the Nrf2-activating properties of a complex coffee beverage, two different model coffees were generated by variation of the roasting conditions: a low-roast coffee rich in CGA and a heavy-roast low in CGA but containing high levels of NMP. Activation of the Nrf2/antioxidant-response element pathway was monitored in vitro and in vivo.

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“…variety of environmental and dietary factors such as garlic organosulfur compounds (44), chemopreventive selenocysteine conjugates (43a), and coffee (42). Hence, GSTP induction by such agents could modify the CVD risk of cigarette smoking.…”

Section: Discussionmentioning

confidence: 99%

Glutathione-S-transferase P protects against endothelial dysfunction induced by exposure to tobacco smoke

Conklin¹,

Haberzettl²,

Prough³

et al. 2009

American Journal of Physiology-Heart and Circulatory Physiology

100

101

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Coffee consumption induces GSTP in plasma and protects lymphocytes against (±)-anti-benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide induced DNA-damage: Results of controlled human intervention trials

Cited by 66 publications

References 66 publications

Intervention with polyphenol‐rich fruit juices results in an elevation of glutathione S‐transferase P1 (hGSTP1) protein expression in human leucocytes of healthy volunteers

Intervention with polyphenol‐rich fruit juices results in an elevation of glutathione S‐transferase P1 (hGSTP1) protein expression in human leucocytes of healthy volunteers

Coffees rich in chlorogenic acid or N‐methylpyridinium induce chemopreventive phase II‐enzymes via the Nrf2/ARE pathway in vitro and in vivo

Glutathione-S-transferase P protects against endothelial dysfunction induced by exposure to tobacco smoke

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