Coffea arabica Bean Extracts and Vitamin C: A Novel Combination Unleashes MCF-7 Cell Death

Abstract

Background:: Vitamin C (VC) is believed to enhance immunity and is regularly integrated as a supplementary agent during several treatments. Objective:: The green (GC) and roasted (RC) coffee (Coffea arabica) aqueous extracts (0, 125, 250 and 500 μg/ml) combined with VC (50 μg/ml) were examined on the cancerous MCF-7 cell line and normal human lymphocytes. Methods:: Neutral red uptake assay, comet assay, immunocytochemical reactivity for protein expression and mRNA expression of apoptosis-related genes were performed. Results:: A significant (P< 0.05) concentration-dependent increase of apoptotic features, such as morphological changes, and abundant nuclear condensation, altered the expression of p53 and caspase-3 mRNA, down-regulation of Bcl-2 protein as well as the acidic autophagosomal vacuolization in treated cells. The oxidative stress and DNA single-strand breaks were noticed too. Conclusion:: These results suggest that coffee in combination with VC undergoes apoptotic anticancer pathway. This supports the integration of coffee and VC as a valuable candidate for anticancer research and treatments.

In Vitro Antigenotoxic, Antihelminthic and Antioxidant Potentials Based on the Extracted Metabolites from Lichen, Candelariella vitellina

“…Several herbal extracts showed promising potent scolicidal and antischistosomal effects [42,43]. The herbs' protective potentials against various toxic agents [44][45][46][47], anticancer activities towards many types of cancers and anti-inflammatory properties [48][49][50][51][52][53][54] were also reported in vivo and in vitro.…”

Progress in Phytochemical and Bioactivities of Coffea arabica L.

“…Ratanamarno et al [5] reported that catechin (C, 6), epicatechin (EC, 7), epicatechin gallate (ECG, 8) and epigallocatechin gallate (EGCG, 9) in fresh C. arabica L. leaves. Chen et al identified delphinidin 3,5-diglucoside (10) and delphinidin 3-(6"-malonyl-glucoside) (11) in the young leaves of C. arabica L. [6] Besides the above mentioned constituents, flavonoids and their glycosides were also isolated from the leaves of C. arabica L., which were named as cyanidin-3-O-Glu (12), cyanidin-3-O-rutinoside (13), kaempferol (14), kaempferol-3-O-Glc (15), kaempferol-3-O-Glc-Hex-DeHex (16), kaempferol-3-O-Glc-Hex (17), kaempferol-3-O-Glc-(6''-Rha) (18), quercetin (19), quercitrin (20), isoquercitrin (21), rutin (22), hyperoside (23), quercetin-3-O-Glc-Hex-DeHex (24), quercetin-3-O-Glu (25), luteolin (26), patuletin (27), fisetin (28), myricetin (29) and apigenin (30). [6][7][8][9] Their structures are shown in Figure 2.…”

“…According to a new study by Ei-Garawani, apoptotic anticancer pathway of the green and roasted C. arabica L. aqueous extracts combined with VC was examined on the cancerous MCF-7 cell line and normal human lymphocytes. [20] Antidiabetic activity Sake et al [21] found that C. arabica L. bean and leaf extract showed a high decrease in blood glucose levels compared to the control group. Mellbye et al [22] found cafestol could increase glucose-stimulated insulin secretion in vitro and increase glucose uptake in human skeletal muscle cells.…”

Imidacloprid Induces Oxidative Stress and Genotoxicity in Nile Tilapia: The Role of Ascorbic Acid Combined Exposure

“…In Prochilodus lineatus sh, MN and DNA damage were evidenced in the erythrocytes as a result of Imid exposure 6,24 . Vitamin C, chemically known as ascorbic acid (Asc), is well known for its strong antioxidant potency [25][26][27] . It is a good reducing agent and inhibits lipid peroxidation 28 , And even in small doses, its effectiveness for redox recycling was proved 29 .…”