Coexpression of Two mRNA Isoforms of Insulin-Like Growth Factor-1 Gene and mRNA of YB-1 Gene in Patients with Multiple Myeloma

Шушанов, С. С.; Марьина, Л. Г.; Кравцова, Т. А.; Chernykh, Yu. B.; Kakpakova, E. S.

doi:10.1007/s10517-013-2023-6

Cited by 3 publications

(4 citation statements)

References 11 publications

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“…Hence, mRNA expression of insulin receptor only A isoform was characteristic of the studied MM cells. These results completely coincided with our previous data indicating the expression of mainly IRA mRNA in clinical samples from MM patients [1].…”

Section: Resultssupporting

confidence: 92%

“…According to some data, stimulation of IRA mediates proliferative effects, while activation of IRB mediates metabolic effects [7,14]. In a previous study [1], we have focused on IRA and IRB mRNA expression in aspirated bone marrow specimens from patients treated for stage III multiple myeloma (MM). These samples express mainly IRA mRNA (in 100% cases), while the expression of IRB mRNA is found in only 32% patients.…”

mentioning

confidence: 99%

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Insulin Effects on Survival of Human Multiple Myeloma Cells

Шушанов¹,

Кравцова²,

Chernykh³

et al. 2015

Bull Exp Biol Med

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Insulin effects of human multiple myeloma cell survival were studied on RPMI1640, RPMI8226, and IM9 lines differing by differentiation degree. The effects of exogenous insulin on tumor cell growth and survival varied. Insulin alone did not improve the viability of myeloma cells, while in combination with serum growth factors increased it. The IM9 cells with immunophenotype (CD(138+), CD(38-), CD(45+), CD(56-), CD(19+)) exhibited the highest sensitivity to serum growth factors, while RPMI1640 and RPMI8226 cells with (CD(138+), CD(38+), CD(45-), CD(56±), CD(19-)) immunophenotype were less sensitive. Studies of gene expression showed a significantly lower level of IRA mRNA expression in IM9 vs. RPMI1640 and RPMI8226 cells.

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Section: Resultssupporting

confidence: 92%

mentioning

confidence: 99%

Insulin Effects on Survival of Human Multiple Myeloma Cells

Шушанов¹,

Кравцова²,

Chernykh³

et al. 2015

Bull Exp Biol Med

Self Cite

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“…In the present study, HIF1α and IGF1 mRNA expression levels were significantly increased in RPMI-8226 and primary myeloma cells compared with those in healthy donor cells. The results have confirmed the data of previous studies (3)(4)(5)10,39), which have suggested that high levels of HIF1α and IGF1 contribute to angiogenesis and promote MM disease progression.…”

Section: Discussionsupporting

confidence: 90%

Rosiglitazone suppresses angiogenesis in multiple myeloma via downregulation of hypoxia-inducible factor-1α and insulin-like growth factor-1 mRNA expression

Rui

Huang

Liu

et al. 2014

Molecular Medicine Reports

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Rosiglitazone (RGZ) is a thiazolidinedione ligand of peroxisome proliferator-activated receptor-γ. Our previous studies have confirmed that RGZ possesses antitumoral properties. Bone marrow angiogenesis exhibits an important role in multiple myeloma (MM), and angiogenesis often correlates with the prognosis and disease burden of MM. However, to the best of our knowledge, inhibition of angiopoiesis by RGZ in MM has not yet been reported. The present study aimed to investigate whether RGZ prevents angiogenesis and the possible underlying mechanism of this effect in MM. RPMI‑8226 cells, primary myeloma cells from patients with MM or mononuclear cells from healthy patients were treated with different concentrations of RGZ, and various biological responses were detected using MTT, reverse transcription-polymerase chain reaction and western blot assays. The expression levels of hypoxia-inducible transcription factor‑1α (HIF1α) and insulin-like growth factor‑1 (IGF1) were significantly increased in the RPMI-8226 cells and the primary myeloma cells from the patients with MM compared with those in the mononuclear cells from the healthy patients. The results also showed that RGZ was able to inhibit proliferation and reduce viability of RPMI-8226 cells in a concentration-and time-dependent manner. RGZ was able to concentration‑dependently inhibit the expression of HIF1α and IGF1 mRNA in RPMI-8226 and primary myeloma cells from patients with MM. RGZ also inhibited the expression of pAKT and downregulated the expression levels of phosphorylated extracellular signal-regulated kinase (ERK) in RPMI-8226 cells. The results suggested that RGZ inhibits the angiopoiesis of tumors by interfering with the phosphatidylinositol 3-kinase/AKT and ERK signaling pathways.

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“…In addition, we documented an increased expression of IGF-IEc isoform in secondary compared to primary foci of neuroendocrine neoplasms (18). Other investigators have reported the overexpression of IGF-IEa and IGF-IEb in multiple myeloma patients (41) and of IGF-IEb in uterine cancer (23).…”

Section: Discussionsupporting

confidence: 71%