Coexpression of tissue factor and tissue factor pathway inhibitor by human monocytes purified by leukapheresis and elutriation.Response of nonadherent cells to lipopolysaccharide

Nguyên, Philippe; Broussas, Matthieu; Cornillet‐Lefèbvre, Pascale; Potron, G

doi:10.1046/j.1537-2995.1999.39090975.x

Cited by 26 publications

(20 citation statements)

References 43 publications

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“…After density sedimentation of the mononuclear cells with lymphocyte separation medium (Organon, Teknika, Durham, NC), the monocytes were purified by counterflow centrifugal elutriation, as described previously [25], except that pyrogen-free PBS was used in the elutriation procedure. Monocytes were enriched >90% as determined by morphology, non-specific esterase staining, and flow cytometry.…”

Section: Methodsmentioning

confidence: 99%

Induction of IL-12 Production in Human Peripheral Monocytes byTrypanosoma cruziIs Mediated by Glycosylphosphatidylinositol-Anchored Mucin-Like Glycoproteins and Potentiated by IFN-γand CD40-CD40L Interactions

Abel

Ferreira

Navarro

et al. 2014

Mediators of Inflammation

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Chagas disease, caused by the protozoan parasite Trypanosoma cruzi (T. cruzi), is characterized by immunopathology driven by IFN-γ secreting Th1-like T cells. T. cruzi has a thick coat of mucin-like glycoproteins covering its surface, which plays an important role in parasite invasion and host immunomodulation. It has been extensively described that T. cruzi or its products—like GPI anchors isolated from GPI-anchored mucins from the trypomastigote life cycle stage (tGPI-mucins)—are potent inducers of proinflammatory responses (i.e., cytokines and NO production) by IFN-γ primed murine macrophages. However, little is known about whether T. cruzi or GPI-mucins exert a similar action in human cells. We therefore decided to further investigate the in vitro cytokine production profile from human mononuclear cells from uninfected donors exposed to T. cruzi as well as tGPI-mucins. We observed that both living T. cruzi trypomastigotes and tGPI-mucins are potent inducers of IL-12 by human peripheral blood monocytes and this effect depends on CD40-CD40L interaction and IFN-γ. Our findings suggest that the polarized T1-type cytokine profile seen in T. cruzi infected patients might be a long-term effect of IL-12 production induced by lifelong exposure to T. cruzi tGPI-mucins.

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Section: Methodsmentioning

confidence: 99%

Induction of IL-12 Production in Human Peripheral Monocytes byTrypanosoma cruziIs Mediated by Glycosylphosphatidylinositol-Anchored Mucin-Like Glycoproteins and Potentiated by IFN-γand CD40-CD40L Interactions

Abel

Ferreira

Navarro

et al. 2014

Mediators of Inflammation

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“…In addition to endothelial cells, many other vascular wall cells can synthesize TFPI, e.g., smooth muscle cells, monocytes, fibroblasts, and cardiomyocytes [24][25][26][27][28]. In order to make sure whether MsC in glomeruli synthesizes TFPI, we investigated the expression of TFPI in cultured rat mesangial cells by immunofluorescence.…”

Section: Tfpi Expression Changes In Thy1 Gnmentioning

confidence: 99%

Recombinant tissue factor pathway inhibitor induces apoptosis in cultured rat mesangial cells via its Kunitz-3 domain and C-terminal through inhibiting PI3-kinase/Akt pathway

et al. 2007

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Tissue factor pathway inhibitor (TFPI) is an endogenous inhibitor of tissue factor (TF) induced coagulation. In addition to its anticoagulation activity, TFPI has other functions such as antiproliferation and inducing apoptosis. In the present study, we investigated whether or not TFPI induced apoptosis in cultured rat mesangial cells (MsCs) and the possible signal pathway that involved in the apoptotic process. We demonstrated that recombinant TFPI (rTFPI) induced apoptosis in cultured MsCs via its Kunitz-3 domain and C-terminal in a dose- and time-dependent manner by Hoechst 33258 assay, flow cytometry, nucleosomal laddering of DNA, caspase 3 assay. Because the serine/threonine protein kinase Akt has attracted attention as a mediator of survival (anti-apoptotic) signal in MsCs, we investigated the expression of phosphospecific-Akt and its downstream signal phospho-IkappaB-alpha and some other signal molecules like Fas and bcl-2. The results indicated that the process of apoptosis triggered by rTFPI is, at least in part, actively conducted by rat MsCs possibly through PI3-Kinase-Akt signal pathway not by binding to tissue factor. Our findings suggest that rTFPI has the potential usefulness in inducing apoptosis of MsCs under inflammatory conditions.

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“…Informed consent was obtained from each participant. Human monocytes were purified from cytapheresis material by elutriation, as previously described [19]. Monocyte purity was assessed by CD14 staining of isolated cells ([95% CD14-positive), and cell viability ([98%) was determined by the trypan blue exclusion principle.…”

Section: Monocyte Purificationmentioning

confidence: 99%

IL-10 modulates fondaparinux inhibition of monocyte-induced thrombin generation

Ben-Hadj-Khalifa

Hézard

Almawi

et al. 2011

J Thromb Thrombolysis

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In addition to its established immuno-regulating capacity, the anti-inflammatory cytokine interleukin (IL)-10 exerts direct effects on coagulation. IL-10 down regulates the expression of tissue factor (TF) and thrombin generation (TG). Thus, we hypothesised that IL-10 could enhance the effect of anticoagulants. To evaluate in vitro the potential additive effect of IL-10 on fondaparinux-induced anticoagulation. Human monocytes were purified by elutriation, and were activated by factor Xa (FXa). Real-time RT-PCR and Western blotting were used to evaluate FXa-induced TF synthesis. TG test was used as a functional test to assess TF-dependent monocyte procoagulation, and to evaluate the effects of IL-10 (200 and 500 pg/ml) and fondaparinux (0.0, 0.1, 0.4, 0.7 and 1.2 μg/ml), separately and in combination. We confirmed that FXa induced TF mRNA and protein synthesis by monocyte in a concentration dependent manner. We showed that FXa-activated monocytes triggered TG via TF expression. We reported that IL-10 inhibited TG with a marginal effect seen at 200 pg/ml. Results with fondaparinux showed a concentration-dependent TG inhibition. The combination of IL-10 and fondaparinux effects demonstrated that IL-10: (i) potentiates the inhibitory effect of fondaparinux on TG by 10-30%, and (ii) dramatically modifies fondaparinux IC50 for each TG parameter. IL-10 enhances in vitro the extent of anticoagulation induced by fondaparinux.

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Coexpression of tissue factor and tissue factor pathway inhibitor by human monocytes purified by leukapheresis and elutriation.Response of nonadherent cells to lipopolysaccharide

Abstract: In spite of the coexpression of TF mRNA and TFPI mRNA, elutriated monocytes are capable of supporting prothrombinase activity. This should be taken into account in the evaluation of the safety of adoptive cellular immunotherapy.

Cited by 26 publications

References 43 publications

Induction of IL-12 Production in Human Peripheral Monocytes byTrypanosoma cruziIs Mediated by Glycosylphosphatidylinositol-Anchored Mucin-Like Glycoproteins and Potentiated by IFN-γand CD40-CD40L Interactions

Induction of IL-12 Production in Human Peripheral Monocytes byTrypanosoma cruziIs Mediated by Glycosylphosphatidylinositol-Anchored Mucin-Like Glycoproteins and Potentiated by IFN-γand CD40-CD40L Interactions

Recombinant tissue factor pathway inhibitor induces apoptosis in cultured rat mesangial cells via its Kunitz-3 domain and C-terminal through inhibiting PI3-kinase/Akt pathway

IL-10 modulates fondaparinux inhibition of monocyte-induced thrombin generation

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