Coexpression of thyroid hormone receptor isoforms in mouse oligodendrocytes

Abstract

Double and triple immunocytochemistry with stage-specific markers and specific antireceptor antibodies was used to study expression of nuclear thyroid hormone receptor (TR) isoforms in cultured mouse oligodendrocytes. To evaluate the coexpression of each TR isoform, antibodies were raised in rabbits and mice against specific regions of alpha1-TR and alpha2-TR common to both alpha isoforms and beta1-TR. Their specificities were assessed by Western blotting and by immunocytochemistry on rat hepatocytes. Oligodendrocyte subpopulations were found to coexpress the alpha- and beta1-TR epitopes at defined developmental stages. Both alpha- and beta1-TR isoforms are colocalized in oligodendrocytes during an early stage identified by the marker OL-1, before 2',3'-cyclic nucleotide 3'-phosphohydrolase is expressed. Expression of beta1-TR varies during maturation, and that of alpha-TR decreases during terminal maturation.

Transferrin and thyroid hormone converge in the control of myelinogenesis

“…There appears to be general consensus that both OPCs and OLGs express TRα and that OLGs express TRβ (Carré et al, 1998). According to Bury et al (2002), TRα and TRβ colocalize in OLGs before CNPase is expressed. Thus, it might be speculated that, during the initial stages of OLG differentiation, Tf triggers TRα expression in the SVZ and promotes undifferentiated proliferating cells to become responsive to this trophic factor.…”

A selective thyroid hormone β receptor agonist enhances human and rodent oligodendrocyte differentiation

“…While some groups (Baas et al, 1994a; Baas et al, 1994b; Billon et al, 2002; Billon et al, 2001; Bury et al, 2002) report expression in differentiated OLs but not OPCs, others have found THRβ1 to be present in both cell types (Barres et al, 1994). These discrepant findings may be explained by changes in the expression of THRβ1 within OPCs that are dependent on the “age” of the cell.…”

Aorta-Derived Mesoangioblasts Differentiate into the Oligodendrocytes by Inhibition of the Rho Kinase Signaling Pathway

“…*A statistically significant difference between these values in the figure. Color images available online at www .liebertonline.com/scd progressively decreases as OP cells mature into oligodendrocytes, and it is therefore primarily a marker of OP cells [63]. TRa was detected in the nucleus of ADM cultured in GM, in a punctate staining pattern that colocalized with DAPI (Fig.…”