Coexpression of ER  with ER  and Progestin Receptor Proteins in the Female Rat Forebrain: Effects of Estradiol Treatment

Greco, Béatrice

doi:10.1210/en.142.12.5172

Cited by 106 publications

(106 citation statements)

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“…Moreover, variation in the expression of ER-b was not found to be dependent on the concentration of a circulating gonadal steroid (Greco et al 2001). found that the expression of ER-b is negatively correlated with changes in plasma osmolality in males.…”

Section: Discussionmentioning

confidence: 90%

Effect of the interaction between food state and the action of estrogen on oxytocinergic system activity

Lucio-Oliveira¹,

Franci²

2011

Journal of Endocrinology

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Increased plasma osmolality by food intake evokes augmentation of plasma oxytocin (OT). Ovarian steroids may also influence the balance of body fluids by acting on OT neurones. Our aim was to determine if estrogen influences the activity of OT neurones in paraventricular nucleus (PVN) and supraoptic nucleus (SON) under different osmotic situations. Ovariectomized rats (OVX) were treated with either estradiol (E 2 ) or vehicle and were divided into three groups: group I was fed ad libitum, group II underwent 48 h of fasting, and group III was refed after 48 h of fasting. On the day of the experiment, blood samples were collected to determine the plasma osmolality and OT. The animals were subsequently perfused, and OT/FOS immunofluorescence analysis was conducted on neurones in the PVN and the SON. When compared to animals which were fasted or fed ad libitum, the plasma osmolality of refed animals was higher, regardless of whether they were treated with vehicle or E 2 . We observed neural activation of OT cells in vehicle-or E 2 -treated OVX rats refed after 48 h of fasting, but not in animals fed ad libitum or in animals that only underwent 48 h of fasting. Finally, the percentage of neurones that co-expressed OT and FOS was lower in both the PVN and the SON of animals treated with E 2 and refed, when compared to vehicle-treated animals. These results suggest that E 2 may have an inhibitory effect on OT neurones and may modulate the secretion of OT in response to the increase of osmolality induced by refeeding.

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Section: Discussionmentioning

confidence: 90%

Effect of the interaction between food state and the action of estrogen on oxytocinergic system activity

Lucio-Oliveira¹,

Franci²

2011

Journal of Endocrinology

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“…The ER subtypes have unique roles in estrogen-dependent gene regulation and the transcriptional activities of ERα and ERβ differ depending on the cell and tissue context (Hall and McDonnell, 1999). The majority of tissues express predominantly one ER subtype over another, however expression of both ERα and ERβ in the same cell type has been reported for instance in neurons and thymocytes (Greco et al, 2001;Mor et al, 2001). Therefore the presence of both subtypes would predict a more complex regulation of estrogen-mediated gene expression.…”

Section: Introductionmentioning

confidence: 99%

Subtype specific estrogen receptor action protects against changes in MMP-2 activation in mouse retinal pigmented epithelial cells

Elliot

Catanuto

Fernández

et al. 2008

Experimental Eye Research

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Eyes with age-related macular degeneration (AMD) demonstrate accumulation of specific deposits and extracellular matrix (ECM) molecules under the retinal pigment epithelium (RPE). AMD is about two times more prevalent in aging postmenopausal women. Therefore we studied whether 17β-estradiol (E 2 ) modulates the expression and activity of the trimolecular complex (MMP-2, TIMP-2 and MMP-14), molecules which are of major importance for ECM turnover in RPE. We used cell lines isolated from estrogen receptor knockout mice (ERKO) to determine which ER (estrogen receptor) subtype was important for ECM regulation in RPE cells.We found that mouse RPE sheets had higher baseline MMP-2 activity in the presence of ERβ. This correlated with higher MMP-2 activity in RPE cell lines isolated from ERKOα mice. Exposure to E 2 increased MMP-2 activity in mouse RPE cell lines. In addition E 2 increased transcriptional activation of the MMP-2 promoter through a functional Sp1 site which required the presence of ERβ, but not ERα. E 2 also maintained levels of pro MMP-2, and MMP-14 and TIMP-2 activity after oxidant injury. Since the direct effects of E 2 on MMP-2 transcriptional activation and the regulation of the trimolecular complex after oxidant induced injury requires ERβ, this receptor subtype may have a role as a potential therapeutic target to prevent changes in activation of MMP-2.

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“…Therefore, one possibility is that the protein-protein interactions required for ER␤ signaling are altered with age and the bioavailability of estrogens. Previous studies have shown that ER␤ can associate with traditional coregulators in the brain such as steroid coactivator-1 and estrogen receptor associated protein 140 (5)(6)(7), and that these associations are modified by multiple factors, including age. For instance, one study demonstrated decreased association between estrogen receptor associated protein 140 and ER␤ in the aged hippocampus, despite an overall increase in estrogen receptor associated protein 140 expression (6).…”

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confidence: 99%

Age-dependent Effects of 17β-estradiol on the Dynamics of Estrogen Receptor β (ERβ) Protein–Protein Interactions in the Ventral Hippocampus

Mott

Pinceti

Rao

et al. 2014

Molecular & Cellular Proteomics

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Recent clinical evidence suggests that the neuroprotective and beneficial effects of hormone therapy may be limited by factors related to age and reproductive status. The patient's age and length of time without circulating ovarian hormones are likely to be key factors in the specific neurological outcomes of hormone therapy. However, the mechanisms underlying age-related changes in hormone efficacy have not been determined. We hypothesized that there are intrinsic changes in estrogen receptor ␤ (ER␤) function that determine its ability to mediate the actions of 17␤-estradiol (E2) in brain regions such as the ventral hippocampus. In this study, we identified and quantified a subset of ER␤ protein interactions in the ventral hippocampus that were significantly altered by E2 replacement in young and aged animals, using two-dimensional differential gel electrophoresis coupled with liquid chromatography-electrospray ionization-tandem mass spectrometry. This study demonstrates quantitative changes in ER␤ protein-protein interactions with E2 replacement that are dependent upon age in the ventral hippocampus and how these changes could alter processes such as transcriptional regulation. Thus, our data provide evidence that changes in ER␤ protein interactions are a potential mechanism for age-related changes in E2 responsiveness in the brain after menopause. Molecular & Cellular Proteomics 13: 10.1074/mcp.M113.031559, 760-779, 2014.The neuroprotective and beneficial effects of estrogens in the brain have been reported for decades, yet recent evidence from clinical trials suggests that the benefits of estrogens in postmenopausal women might not outweigh the risks (1-3). Specifically, the risk of cardiovascular disease and invasive breast cancer was significantly increased in postmenopausal women given hormone therapy as part of the largest clinical trial performed to date (Women's Health Initiative). These results sharply contradicted substantial evidence from numerous studies in animal models, prompting a reevaluation of the data from the Women's Health Initiative studies. Later it was determined that factors contributing to the observed detrimental effects of hormone therapy in the Women's Health Initiative study included advanced age, the types of synthetic estrogens and progestins used in the study, and, perhaps most important, the number of years postmenopause prior to the initiation of hormone therapy (4). However, more than 10 years after the conclusion of these studies there is little to no mechanistic explanation for how aging contributes to a change in estrogen signaling.One possibility is that there are age-related changes in the way the brain responds to estrogens. We hypothesized that there are intrinsic changes in the function of estrogen receptors in the brain with advanced age, and estrogen receptor ␤ (ER␤) 1 in particular has been shown to be a critical regulator of many neurobiological functions. An important component of ER␤ signaling is requisite associations with intracellular coregulatory proteins. ...

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Coexpression of ER with ER and Progestin Receptor Proteins in the Female Rat Forebrain: Effects of Estradiol Treatment

Cited by 106 publications

References 0 publications

Effect of the interaction between food state and the action of estrogen on oxytocinergic system activity

Effect of the interaction between food state and the action of estrogen on oxytocinergic system activity

Subtype specific estrogen receptor action protects against changes in MMP-2 activation in mouse retinal pigmented epithelial cells

Age-dependent Effects of 17β-estradiol on the Dynamics of Estrogen Receptor β (ERβ) Protein–Protein Interactions in the Ventral Hippocampus

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