Coexpression of Adrenomedullin and Its Receptors in the Reproductive System of the Rat: Effects on Steroid Secretion in Rat Ovary1

Li, Yuk-Yin; Li, Lei; Hwang, Isabel S.S.; Tang, Florence; Wai‐Sum, O

doi:10.1095/biolreprod.107.064022

Cited by 27 publications

(33 citation statements)

References 38 publications

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“…We have demonstrated a high expression of ADM peptide and mRNA (Li et al 2008) and an action of ADM on ciliary beat in the rat oviduct. This suggests that ADM is a potential factor to affect oviductal function.…”

Section: Introductionmentioning

confidence: 78%

Adrenomedullin increases ciliary beat frequency and decreases muscular contraction in the rat oviduct

Liao

Ho²,

Tang³

2011

Reproduction

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Our laboratory previously showed that oviduct produced the greatest amount of adrenomedullin (ADM) in the rat female reproductive tract. The aim of this study is to investigate the changes in ADM levels resulting from the contact between the sperm and the oviduct and the possible roles of ADM in ciliary beating and oviductal contractility. Oviducts from Sprague-Dawley rats removed at pre-and post-ovulatory stages were cut open longitudinally and treated with ADM and/or receptor blockers before ciliary beat frequency (CBF) was measured. The effects of sperm on ADM production and CBF in the oviduct were also determined. The contraction of the oviduct after treatment with ADM and receptor antagonists was measured using the organ-bath technique. The results showed that ADM increased the CBF in rat oviduct and this stimulating effect was blocked by the calcitonin-gene-related peptide (CGRP) receptor antagonist, hCGRP . CBF was lower in post-ovulatory than pre-ovulatory oviducts. The presence of sperm in the oviduct increased both the ADM level and CBF. ADM treatment was shown to inhibit the contractility of the oviduct by lowering the basal tone and decreasing the contraction amplitude. The ADM receptor antagonist, hADM , was effective in counteracting the relaxation effect of ADM in the oviduct. All in all, these results indicate that ADM may play a crucial role in transporting the gametes/embryos by regulating ciliary beating and muscular contraction.

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Section: Introductionmentioning

confidence: 78%

Adrenomedullin increases ciliary beat frequency and decreases muscular contraction in the rat oviduct

Liao

Ho²,

Tang³

2011

Reproduction

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“…Studies of ADM and CGRP in rat ovaries have shown that although CGRP is confined to nerve endings [141, 142], ADM expression in ovarian follicles increases from the small antral stage to the large antral stage and corpora lutea. In addition, levels of ADM in ovarian follicles and the corpora lutea vary during the estrous cycle [17–19, 21], and the ADM level in the corpus luteum is higher in late pregnancy compared to early pregnancy [24]. Although CGRP appeared to have no effect on the basal or follicle-stimulating hormone (FSH)-stimulated release of estradiol or progesterone from cultured rat granulosa cells [141], ADM and IMD stimulated cAMP and estradiol production in cultured granulosa cells [143] (Fig.…”

Section: Role Of Clr/ramp Receptor Signaling In the Ovarymentioning

confidence: 99%

“…ADM, CGRP, IMD, and their receptors are highly conserved across species [13–15], and are expressed in female reproductive organs including the ovaries, oviducts, and uterus as well as the placenta [16–21]. During the evolution of placental reproduction in therian mammals, these con-served peptides and receptors were recruited to perform new regulatory functions that originated in the cardiovascular system.…”

Section: Introductionmentioning

confidence: 99%

Roles of CLR/RAMP Receptor Signaling in Reproduction and Development

Chang¹,

Hsu

2013

CPPS

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Adrenomedullin (ADM), calcitonin gene-related peptides (α- and β-CGRPs), and intermedin/adrenomedullin 2 (IMD/ADM2) are major regulators of vascular tone and cardiovascular development in vertebrates. Recent research into their functions in reproduction has illuminated the role of these peptides and their cognate receptors (calcitonin receptor-like receptor/receptor activity-modifying protein (CLR/RAMP) receptors) in fetal–maternal blood circulation, feto-placental development, female gamete development, and gamete movement in the oviduct. Although ADM family peptides function in a temporally and spatially specific manner in various reproductive processes, they appear to act via a similar set of second messengers, including nitric oxide, cyclic GMP, cyclic AMP, and calcium-activated potassium channels in different tissues. These discoveries supported the view that CLR/RAMP receptors were recruited to perform a variety of newly evolved reproductive functions during the evolution of internal reproduction in mammals. These advances also provided insight into how CLR/RAMP receptor signaling pathways coordinate with other physiological adaptions to accommodate the extra metabolic needs during pregnancy, and captured some important details as to how fetal–maternal vascular communications are generated in the first place. Furthermore, these findings have revealed novel, promising opportunities for the prevention and treatment of aberrant pregnancies such as pregnancy-induced hypertension, preeclampsia, and tubal ectopic pregnancy. However, significant efforts are still needed to clarify the relationships between certain components of the CLR/RAMP signaling pathway and aberrant pregnancies before CLR/RAMP receptors can become targets for clinical management. With this understanding, this review summarizes recent progresses with particular focus on clinical implications.

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“…It has been shown that the presence of sperm up-regulates the expression of oviductal ADM in mice, suggesting a possible physiological role of ADM in this part of the female genital tract (13). Previous work in our group has demonstrated the expression of ADM peptide and mRNA in rat oviduct by immunohistochemistry and RT-PCR (11,14). In humans, our preliminary work (15) also showed that ADM in the human oviduct varied in its levels with the hormonal cycle, stimulated ciliary motility of the human oviduct and was up-regulated by contact with sperm.…”

mentioning

confidence: 88%

Possible Role of Adrenomedullin In the Pathogenesis of Tubal Ectopic Pregnancy

Liao

Li²,

Ho³

et al. 2012

The Journal of Clinical Endocrinology &Amp; Metabolism

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Context: Tubal ectopic pregnancy (tEP) is currently the leading cause of pregnancy-related deaths during the first trimester. Our current knowledge on the molecular pathogenesis is limited. Objective:The objective of the study was to find out the possible role of adrenomedullin (ADM) in the pathogenesis of tEP. Design:This was an experimental in vitro study on oviductal tissue. Setting:The study was conducted at a university teaching hospital. Patients and Interventions:Patients included those having oviducts removed surgically during salpingectomy for tEP or hysterectomy for benign gynecological conditions. Oviductal tissues were incubated in hormonal condition mimicking early pregnancy before used for in vitro experiments. Main Outcome Measures:Plasma ADM concentration, oviductal expression of ADM and its receptors, ciliary beat frequency, smooth muscle contraction were measured. Results:The ciliary beat frequency and frequency of muscle contraction were lower in the oviducts from patients with tEP than those from simulated normal pregnancy. The plasma and oviductal tissue ADM levels were also lower. The decreases in ciliary beat and frequency of contraction were restored to normal after ADM treatment. Conclusions:The results suggest that the lower ADM level in the oviducts of tEP may lead to the decrease in ciliary beating and muscle contraction, with the result that the embryo is retained and implanted in the oviduct. Our findings explain for the first time the etiology of tubal pregnancy on the basis of an impairment of the transport of the fertilized ovum resulting from an ADM deficiency and raise the possibility of using the plasma ADM level as a predictor for tubal ectopic pregnancy. (J Clin Endocrinol Metab 97: 2105-2112, 2012)

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Coexpression of Adrenomedullin and Its Receptors in the Reproductive System of the Rat: Effects on Steroid Secretion in Rat Ovary1

Cited by 27 publications

References 38 publications

Adrenomedullin increases ciliary beat frequency and decreases muscular contraction in the rat oviduct

Adrenomedullin increases ciliary beat frequency and decreases muscular contraction in the rat oviduct

Roles of CLR/RAMP Receptor Signaling in Reproduction and Development

Possible Role of Adrenomedullin In the Pathogenesis of Tubal Ectopic Pregnancy

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