Coexistence of NDM-1-producing Escherichia coli and Citrobacter freundii in the same patient

Zhu, Bo; Chen, Ying; Xu, Hao; Ying, Jingjing

doi:10.1016/j.jgar.2018.04.013

Cited by 9 publications

(3 citation statements)

References 17 publications

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“…This is an important finding. However, even more importantly, our BLASTn analysis showed that IncX3 plasmids harboring various bla NDM genes, including bla NDM–1 (Lü et al, 2018; Zhu et al, 2018), bla NDM–4 (Espedido et al, 2015; Sugawara et al, 2017), bla NDM–5 (Krishnaraju et al, 2015; Zhu et al, 2016; Li et al, 2018; Xie et al, 2018) , bla NDM–6, bla NDM–7 (Pal et al, 2017; Sugawara et al, 2017), bla NDM–13 (Lv et al, 2016), bla NDM–17 (Liu et al, 2017), bla NDM–19 (Liu et al, 2019), bla NDM–20 (Liu Z. et al, 2018), and bla NDM–21 (Liu L. et al, 2018), which have been found in 15 species among Enterobacterales [ K. pneumoniae (Espedido et al, 2015; Krishnaraju et al, 2015) , K. oxytoca (Paskova et al, 2018; Yoon et al, 2018), Klebsiella michiganensis, Klebsiella aerogenes, E. coli (Zhu et al, 2016; Pal et al, 2017; Sugawara et al, 2017; Li et al, 2018; Xie et al, 2018) , C. freundii (Zhu et al, 2018) , S. enteric, Enterobacter hormaechei, Enterobacter cloacae (Lü et al, 2018) , Enterobacter asburiae (Paskova et al, 2018) , Enterobacter xiangfangensis (Paskova et al, 2018) , Cronobacter sakazakii, Raoultella planticola, Raoultella ornithinolytica (Paskova et al, 2018), and Kluyvera intermedia (Paskova et al, 2018)] from 13 different countries around the world thus far. These countries are China (Zhu et al, 2016; Li et al, 2018; Liu Z. et al, 2018; Xie et al, 2018), the Czech Republic (Paskova et al, 2018), Kuwait (Pal et al, 2017), Korea (Yoon et al, 2018), Oman (Pal et al, 2017), United States, Sweden, Myanmar (Sugawara et al, 2017), Vietnam, India (Krishnaraju et al, 2015), Arabian Peninsula (Pal et al, 2017), Canada, and Australia (Espedido et al, 2015).…”

Section: Discussionmentioning

confidence: 92%

Detection and Genomic Characterization of a Morganella morganii Isolate From China That Produces NDM-5

et al. 2019

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The increasing prevalence and transmission of the carbapenem resistance gene bla NDM–5 has led to a severe threat to public health. So far, bla NDM–5 has been widely detected in various species of Enterobacterales and different hosts across various cities. However, there is no report on the bla NDM–5– harboring Morganella morganii. In January 2016, the first NDM-5-producing Morganella morganii L241 was found in a stool sample of a patient diagnosed as recurrence of liver cancer in China. Identification of the species was performed using 16S rRNA gene sequencing. Carbapenemase genes were identified through both PCR and sequencing. To investigate the characteristics and complete genome sequence of the bla NDM–5 -harboring clinical isolate, antimicrobial susceptibility testing, S1 nuclease pulsed field gel electrophoresis, Southern blotting, transconjugation experiment, complete genome sequencing, and comparative genomic analysis were performed. M. morganii L241 was found to be resistant to broad-spectrum cephalosporins and carbapenems. The complete genome of L241 is made up from both a 3,850,444 bp circular chromosome and a 46,161 bp self-transmissible IncX3 plasmid encoding bla NDM–5 , which shared a conserved genetic context of bla NDM–5 (ΔIS 3000 -ΔIS Aba125 -IS 5 - bla NDM–5 - ble - trpF - dsbC -IS 26 ). BLASTn analysis showed that IncX3 plasmids harboring bla NDM genes have been found in 15 species among Enterobacterales from 13 different countries around the world thus far. In addition, comparative genomic analysis showed that M. morganii L241 exhibits a close relationship to M. morganii subsp. morganii KT with 107 SNPs. Our research demonstrated that IncX3 is a key element in the worldwide dissemination of bla NDM - 5 among various species. Further research will be necessary to control and prevent the spread of such plasmids.

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Section: Discussionmentioning

confidence: 92%

Detection and Genomic Characterization of a Morganella morganii Isolate From China That Produces NDM-5

et al. 2019

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“…In C. freundii strains, bla NDM-1 was found to be carried by diverse plasmids, including IncX3, IncA/C, IncHI1, IncL/M, IncFII, and IncN1. 5 , 13 , 19 , 26–29 In Cf7308, bla NDM-1 was carried on an IncN1 plasmid identical to pNDM-BTR from a local E. coli isolate and pNDM-CWH001 from a C. freundii isolate in Wuhan city. The closely related bla NDM-1 -carrying IncN1 plasmids in E. coli and C. freundii suggested interspecies or intraspecies horizontal transfer has occurred in China.…”

Section: Discussionmentioning

confidence: 99%

Genetic Characterization of blaNDM-1-Carrying Citrobacter portucalensis Sequence Type 328 and Citrobacter freundii Sequence Type 98

Wang¹,

Li²,

Xiao³

et al. 2022

IDR

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“…In this overall scenario, reports of patients simultaneously infected and/or colonized with multiple species of CPE are becoming a source of real concern. Several cases of interspecies exchange of identical bla KPC - [7,8], bla OXA-48 - [9,10], and bla NDM-1 -carrying plasmids have been described [9,[11][12][13]. In particular, those involving the bla NDM-1 were mainly due to the horizontal spread of broad-host-range IncC plasmids (formerly IncA/C2) [14].…”

Section: Introductionmentioning

confidence: 99%

A Patient With Multiple Carbapenemase Producers Including an Unusual Citrobacter sedlakii Hosting an IncC blaNDM-1- and armA-carrying Plasmid

Moser

Keller

Campos-Madueno

et al. 2021

PAI

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Background. Patients colonized with multiple species of carbapenemase-producing Enterobacterales (CPE) are increasingly observed. This phenomenon can be due to the high local prevalence of these pathogens, the presence of important host risk factors, and the great genetic promiscuity of some carbapenemase genes. Methods. We analyzed 4 CPE (Escherichia coli, Klebsiella pneumoniae, Providencia stuartii, Citrobacter sedlakii), 1 extended-spectrum cephalosporin-resistant K. pneumoniae (ESC-R-Kp), and 1 carbapenemase-producing Acinetobacter baumannii simultaneously isolated from a patient transferred from Macedonia. Susceptibility tests were performed using a microdilution MIC system. The complete genome sequences were obtained by using both short-read and long-read whole-genome sequencing technologies. Results. All CPE presented high-level resistance to all aminoglycosides due to the expression of the armA 16S rRNA methylase. In C. sedlakii and E. coli (ST69), both the carbapenemase blaNDM-1 and armA genes were located on an identical IncC plasmid of type 1a. The K. pneumoniae (ST268) and P. stuartii carried chromosomal blaNDM-1 and blaOXA-48, respectively, while the ESC-R-Kp (ST395) harbored a plasmid-located blaCTX-M-15. In the latter 3 isolates, armA-harboring IncC plasmids similar to plasmids found in C. sedlakii and E. coli were also detected. The A. baumannii strain possessed the blaOXA-40 carbapenemase gene. Conclusions. The characterization of the genetic organization of IncC-type plasmids harbored by 3 different species from the same patient offered insights into the evolution of these broad- host-range plasmids. Moreover, we characterized here the first complete genome sequence of a carbapenemase-producing C. sedlakii strain, providing a reference for future studies on this rarely reported species.

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Coexistence of NDM-1-producing Escherichia coli and Citrobacter freundii in the same patient

Cited by 9 publications

References 17 publications

Detection and Genomic Characterization of a Morganella morganii Isolate From China That Produces NDM-5

Detection and Genomic Characterization of a Morganella morganii Isolate From China That Produces NDM-5

Genetic Characterization of blaNDM-1-Carrying Citrobacter portucalensis Sequence Type 328 and Citrobacter freundii Sequence Type 98

A Patient With Multiple Carbapenemase Producers Including an Unusual Citrobacter sedlakii Hosting an IncC blaNDM-1- and armA-carrying Plasmid

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