“…Surrounding elements often include chaperones, crystallization domains, mobile elements, transporters, prophages, and virulence factors (Koni and Ellar, 1993 ; Shao et al, 2001 ; Elleuch et al, 2016 ; Adalat et al, 2017 ; Fayad et al, 2020 ; Lechuga et al, 2020 ). Moreover, the arrangement and distribution of such elements across genomes reveal crucial details about toxin functionality, host adaptation, diversification, and evolution of biopesticides (Khasdan et al, 2007 ; Peng et al, 2015 ; Ruffner et al, 2015 ; Fiedoruk et al, 2017 ; Zheng et al, 2017 ; Fayad et al, 2020 ; Wang et al, 2020 ). Hence, a more exhaustive approach would not only detect pesticidal sequences but also enable comparative genomics analysis of the candidate toxin in order to characterize the complete expression unit.…”