Coexistence of Ehrlichia phagocytophila and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from Italy as determined by 16S rRNA gene sequencing

Cinco, Marina; Padovan, D; Murgia, Rossella; Maroli, M.; Frusteri, L.; Heldtander, M.; Johansson, Karl‐Erik; Engvall, Eva

doi:10.1128/jcm.35.12.3365-3366.1997

Cited by 69 publications

(23 citation statements)

References 12 publications

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“…In Italy, the reported B. burgdorferi s.l. prevalence in questing ticks varies considerably among study areas: from 17.5% to 20.6% in Veneto and Friuli Venezia Giulia (Favia et al., ; Rizzoli et al., ; Capelli et al., ); from 1.3% to 40.1% in Trentino Alto Adige (Cinco et al., ; Mantelli et al., ; Pecchioli et al., ; Nazzi et al., ); 18.0% in Lombardy (Pistone et al., ); 18.2% in Liguria (Mannelli et al., ); from 8.7% to 23.9% in Tuscany (Bertolotti et al., ; Selmi et al., ; Tomassone et al., ); 10.4% in Emilia‐Romagna (Corrain et al., ); 27.9% in Lazio (Cinco et al., ); 29,9% in Marche (Pascucci and Cammà, ). It is difficult to compare our results with those reported in the literature given differences in tick species collected, geographic locations and climatic conditions.…”

Section: Discussionmentioning

confidence: 98%

Detection of Invasive Borrelia burgdorferi Strains in North‐Eastern Piedmont, Italy

Pintore

Ceballos

Iulini

et al. 2014

Zoonoses and Public Health

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Impacts• From 2008 to 2011, 11 human cases of Lyme Borreliosis were reported in the Verbano-Cusio-Ossola (VCO) province, north-eastern Piedmont, Italy.• The abundance and altitudinal distribution of ticks in VCO and B. burg dorferi s.l. in these vectors were examined. Phylogenetic analyses using ospC gene sequences were performed and 14 of 34 positive samples formed a clade with ospC reference sequences of human invasive strains.• The study supports the need for providing the right information to the institutions, physicians, forestry workers and visitors to adopt proper precautions during season at risk and to protect public health.

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Section: Discussionmentioning

confidence: 98%

Detection of Invasive Borrelia burgdorferi Strains in North‐Eastern Piedmont, Italy

Pintore

Ceballos

Iulini

et al. 2014

Zoonoses and Public Health

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“…Many investigations in Italy have been conducted in mountain and wild habitats (Cacciapuoti et al., 1995; Cinco et al., 1997, 1998; Ciceroni et al., 1998; Mannelli et al., 1999, 2003; Favia et al., 2001; Rizzoli et al., 2002; Santino et al., 2002, 2003; Bertolotti et al., 2006; Mantelli et al., 2006; Piccolin et al., 2006; Pecchioli et al., 2007). However, there is a lack of studies performed in periurban parks.…”

Section: Discussionmentioning

confidence: 99%

Study on Ticks and Tick‐Borne Zoonoses in Public Parks in Italy

Corrain

Drigo

Fenati

et al. 2012

Zoonoses and Public Health

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A survey on tick density and on tick-borne zoonoses was carried out in four public parks in the outskirts of Imola (northern Italy) from June to October 2006. All stages of Ixodes ricinus and only larvae of Riphicephalus sanguineus were recovered by dragging, performed on 100-m transects. Almost all ticks (99%) were harvested in one park. I. ricinus density (nymphs/100 m(2) ) ranged from 0 in park L to 6.3 in park F. Nymphs and adults of I. ricinus were subjected to PCR for Anaplasma phagocytophilum, Bartonella spp., Borrelia burgdorferi s. l. and Rickettsia spp. The observed prevalences were 38.3% for Bartonella henselae, 5.2% for Bartonella clarridgeiae, 10.4% for B. burgdorferi s. l., 2.6% for Rickettsia helvetica and 13% for Rickettsia monacensis, respectively. No DNA of A. phagocytophilum was found. Acarological risks (AR) were calculated as probabilities of collecting at least one infected nymph per transect. The AR values calculated for the various zoonotic agents were 11.4% for R. helvetica, 27.7% for B. clarridgeiae, 49.7% for B. burgdorferi s. l., 57.2% for R. monacensis and 90.4% for B. henselae, respectively. In this study, B. clarridgeiae was for the first time identified in I. ricinus ticks.

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“…Recent prevalence reports from several countries in Europe described granulocytic Ehrlichia infected free-living I. ricinus ticks in Switzerland, 0.8% [31], in Slovenia, 3.2% [30], or at the east and west coasts of Sweden, 3.1% and 9.2% [36], in a part of Italy, 24% [10], and 2.2% in two studies from southern Germany [6,13,14]. Our data from Thuringia in Middle Germany with 2.3% infected ticks (7 of 305 adult and nymphal I. ricinus ticks) show a close correlation to the described infection rate of 2.2% in Baden-Wu « rtemberg [6] and in Munich [14] in southern Germany.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of granulocytic Ehrlichiae in Ixodes ricinus ticks in Middle Germany (Thuringia) detected by PCR and sequencing of a 16S ribosomal DNA fragment

Hildebrandt

2002

FEMS Microbiology Letters

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A total of 305 Ixodes ricinus ticks (243 nymphs and 62 adults) were collected from three different regions of Thuringia in Middle Germany which are known to be endemic for Borrelia burgdorferi. Our aim was to investigate the carrier rate of ticks for granulocytic Ehrlichia species. The presence of ehrlichial 16S ribosomal DNA was investigated by polymerase chain reaction. Using primers specific for the Ehrlichia phagocytophila group PCR fragments of 151 bp and 943 bp, respectively, were produced in positive samples. Adult ticks showed a significantly higher infection rate (4/62; 6.5%) compared to nymphs (3/243; 1.2%). Prevalence rates varied between 0 and 3.8% regarding the different areas under investigation. The nucleotide sequences showed high similarity (between 97.5% and 99% identity) to the known sequences of the three E. phagocytophila group members HGE agent, E. phagocytophila and Ehrlichia equi. The sequence data did not allow a final classification to a particular member of this group.

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Coexistence of Ehrlichia phagocytophila and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from Italy as determined by 16S rRNA gene sequencing

Cited by 69 publications

References 12 publications

Detection of Invasive Borrelia burgdorferi Strains in North‐Eastern Piedmont, Italy

Detection of Invasive Borrelia burgdorferi Strains in North‐Eastern Piedmont, Italy

Study on Ticks and Tick‐Borne Zoonoses in Public Parks in Italy

Prevalence of granulocytic Ehrlichiae in Ixodes ricinus ticks in Middle Germany (Thuringia) detected by PCR and sequencing of a 16S ribosomal DNA fragment

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