Coevolution of insulin-like growth factors, insulin and their receptors and binding proteins in New World Monkeys

Abstract

Previous work has shown that the evolution of both insulin-like growth factor 1 (IGF1) and insulin shows an episode of accelerated change on the branch leading to New World monkeys (NWM). Here the possibility that this is accompanied by a corresponding episode of accelerated evolution of IGF1 receptor (IGF1R), insulin receptor (IR) and/or IGF binding proteins (IGFBPs) was investigated. Analysis of receptor sequences from a range of primates and some non-primate mammals showed that accelerated evolution did indeed occur on this branch in the case of IGF1R and IR, but not for the similar insulin receptor-related receptor (IRRR) which does not bind insulin or IGF1. Marked accelerated evolution on this branch was also seen for some IGFBPs, but not the mannose 6-phosphate/IGF2 receptor or epidermal growth factor receptor. The rate of evolution slowed before divergence of the lineages leading to the NWM for which sequences are available (Callithrix and Saimiri). For the IGF1R and IR the accelerated evolution was most marked for the extracellular domains (ectodomains).Application of the branch-sites method showed dN/dS ratios significantly greater than 1.0 for both receptor ectodomains and for IGFBP1, and allowed identification of residues likely to have been subject to selection. These residues were concentrated in the N-terminal half of the IGF1R ectodomain but the C-terminal half of the IR ectodomain, which could have implications for the formation of hybrid receptors. Overall the results suggest that adaptive coevolution of IGF1, insulin and their receptors and some IGFBPs occurred during the evolution of NWM. For the most part, the residues that change on this branch could not be associated with specific functional aspects (ligand binding, receptor dimerization, glycosylation) and the physiological significance of this coevolution remains to be established.

An Insulin Receptor-Binding Multifunctional Protein from Tamarindus indica L. Presents a Hypoglycemic Effect in a Diet-Induced Type 2 Diabetes—Preclinical Study

“…Although TTIp 56/287 did not have a similar structure to insulin, it interacted with IR in a different region, and presented an insulin-mimetic action, shown through the preclinical analyses performed. Researchers state that insulin-mimetic plant proteins do not present essentially the same characteristics when compared to each other or to mammalian insulin, as observed in the present study [ 67 , 68 , 69 ]. However, they usually have one point in common: the ability to reduce blood glucose concentrations of laboratory animals with diabetes.…”

Evolution of the Insulin Gene: Changes in Gene Number, Sequence, and Processing

“…These changes, in sequence and function, have been accompanied with an acceleration of the rate of evolution of the insulin protein sequence in the guinea pig and relatives (40,43,44). Similar, but less dramatic, episodes of accelerated evolution of insulin sequences have been observed in some species of New World monkeys (44,45), species that have insulin hormones with lower potency (46).…”

Characterization of a novel alternatively-spliced 5′ exon in the human insulin-like growth factor I (IGF-I) gene, expressed in liver and some cancers

“…Notably, in the two New-World monkeys use of exon 2 predominates (Fig. 6B); the IGF-I system is unusual in this group in several other ways, including accelerated evolution of IGF-I and of its receptor [29].…”