Coevolution of cells and viruses in a persistent infection of foot-and-mouth disease virus in cell culture

Torre, Juan Carlos de la; Martínez‐Salas, Encarnación; Díez, José Manuel Olivares; Villaverde, Antonio; Gebauer, Fátima; Rocha, E; Dávila, Mercedes; Domingo, Esteban

doi:10.1128/jvi.62.6.2050-2058.1988

Cited by 129 publications

(94 citation statements)

References 31 publications

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“…On the other hand, data from FMDV persistent infections in cell cultures have revealed a co-evolution of both cell and resident virus [18] and a rapid phenotypic and genotypic variation of the host cells during the establishment of persistance [19]. Therefore, it has been suggested that a yet unidentified mechanism associated with virus replication could be responsible for this cellular genetic instability [19].…”

Section: Activationmentioning

confidence: 99%

A recombinant foot-and-mouth disease virus antigen inhibits DNA replication and triggers the SOS response in

Benito¹,

Viaplana²,

Corchero³

et al. 1995

FEMS Microbiology Letters

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The 3D gene of foot-and-mouth disease virus encodes the viral RNA dependent RNA polymerase, also called virus infection associated (VIA) antigen, which is the most important serological marker of virus infection. This 3D gene from a serotype C1 virus has been cloned and overexpressed in Escherichia coli under the control of the strong lambda lytic promoters. The resulting 51 kDa recombinant protein has been shown to be immunoreactive with sera from infected animals. After induction of gene expression, an immediate and dramatic arrest of cell DNA synthesis occurs, similar to that produced by genotoxic doses of the drug mitomycin C. This effect does not occur during the production of either a truncated VIA antigen or other related and non-related viral proteins. The inhibition of DNA replication results in a subsequent induction of the host SOS DNA-repair response and in an increase of the mutation frequency in the surviving cells.

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Section: Activationmentioning

confidence: 99%

A recombinant foot-and-mouth disease virus antigen inhibits DNA replication and triggers the SOS response in

Benito¹,

Viaplana²,

Corchero³

et al. 1995

FEMS Microbiology Letters

Self Cite

View full text Add to dashboard Cite

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“…The mechanisms that establish persistent infections in coronaviruses are still not understood; however, as with other RNA viruses, the interplay between virus and host may be the key to initiate persistence, which is then followed by the selection of a mutation to maintain the persistent infection [25][26][27][28]. Although there are some exceptions [24,[28][29]30], the general principle for establishment of virus persistence appears to be the attenuation virus gene expression and a restriction of cytopathic effect [25,29,31]. Whether there are mutations other than the one described above that might contribute to BCoV persistent infection remains to be determined.…”

Section: Introductionmentioning

confidence: 99%

A Leaderless Genome Identified during Persistent Bovine Coronavirus Infection Is Associated with Attenuation of Gene Expression

Liao

2013

PLoS ONE

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The establishment of persistent viral infection is often associated with the selection of one or more mutant viruses. For example, it has been found that an intraleader open reading frame (ORF) in genomic and subgenomic mRNA (sgmRNA) molecules is selected during bovine coronavirus (BCoV) persistence which leads to translation attenuation of the downstream ORF. Here, we report the unexpected identification of leaderless genomes, in addition to leader-containing genomes, in a cell culture persistently infected with BCoV. The discovery was made by using a head-to-tail ligation method that examines genomic 5′-terminal sequences at different times postinfection. Functional analyses of the leaderless genomic RNA in a BCoV defective interfering (DI) RNA revealed that (1) the leaderless genome was able to serve as a template for the synthesis of negative-strand genome, although it cannot perform replicative positive-strand genomic RNA synthesis, and (2) the leaderless genome retained its function in translation and transcription, although the efficiency of these processes was impaired. Therefore, this previously unidentified leaderless genome is associated with the attenuation of genome expression. Whether the leaderless genome contributes to the establishment of persistent infection remains to be determined.

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“…However, the molecular basis of persistence is largely unknown and is additionally complicated by the fact that various mechanisms seem to operate in different cell virus systems (reviewed in Holland, 1990;Oldstone, 1991;Ahmed et al, 1996). Persistently infected cell cultures represent a valuable tool to study the mechanisms of viral persistence and the molecular evolution of viruses and cells (de la Torre et al, 1985(de la Torre et al, , 1988. The latter aspect is especially interesting in the case of single-stranded RNA viruses which lack proofreading and postreplicative error correction mechanisms and are believed to have a high mutation rate (Lai, 1992;Domingo et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Generation of cytopathogenic subgenomic RNA of classical swine fever virus in persistently infected porcine cell lines

Mittelholzer¹,

Moser²,

Tratschin³

et al. 1997

Virus Research

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Two biological clones (A.1 and B.2) of the classical swine fever virus strain Alfort/187 and the recombinant virus vA187-1, derived from a cDNA clone of Alfort/187, were used to establish persistently infected cultures of the swine kidney cell lines SK-6 and PK-41. It was found that 100% of the cells in the passaged cultures were positive for viral antigen throughout the course of the experiment. Additionally, supernatants collected upon passaging of the cells continuously contained high titers of infectious virus. In six separate cultures persistently infected with either the biological clones or the recombinant virus, a cytopathic effect occurred spontaneously between passage 8 and 94. The cytopathogenic agent in the supernatants of these cultures could be passaged repeatedly, suggesting the generation of a mutant virus. Analysis of RNA from such cultures revealed the presence of a subgenomic viral RNA of approximately 8 kilobases (kb). In all six cases, this RNA had an identical internal deletion of 4764 nucleotides, including the region coding for all structural proteins. The subgenomic RNA replicated and was packaged in the presence of wild-type virus. Cells infected with cytopathogenic virus contained increased amounts of the viral protein NS3 thought to be involved in pestivirus cytopathogenicity.

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Coevolution of cells and viruses in a persistent infection of foot-and-mouth disease virus in cell culture

Cited by 129 publications

References 31 publications

A recombinant foot-and-mouth disease virus antigen inhibits DNA replication and triggers the SOS response in

A recombinant foot-and-mouth disease virus antigen inhibits DNA replication and triggers the SOS response in

A Leaderless Genome Identified during Persistent Bovine Coronavirus Infection Is Associated with Attenuation of Gene Expression

Generation of cytopathogenic subgenomic RNA of classical swine fever virus in persistently infected porcine cell lines

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