Coeruleotrigeminal inhibition of nociceptive processing in the rat trigeminal subnucleus caudalis

Tsuruoka, Masayoshi; Matsutani, Kiyo; Maeda, Masako; Inoue, Tomio

doi:10.1016/j.brainres.2003.09.023

Cited by 32 publications

(32 citation statements)

References 39 publications

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“…Electrical stimulation of the LC/SC was delivered at a stimulus intensity up to 50 lA in the present study. Under these conditions, it has been shown that LC/SC stimulation does not spread to nuclei or axons outside the LC/SC [19]. In the present study, LC/SC stimulation never produced contraction of the masseter muscle associated with stimulation .…”

Section: Discussioncontrasting

confidence: 57%

“…At these stimulus intensities, there was no trouble with electrical artifacts in relation to neuronal response measurements during LC/SC stimulation. In addition, LC/ SC stimulation never induced contraction of the masseter muscle associated with stimulation of the mesencephalic trigeminal nucleus, located just lateral to the LC/SC, suggesting that LC/SC stimulation may not have spread to nuclei or axons outside the LC/SC [19].…”

Section: Lc/sc Stimulationmentioning

confidence: 88%

“…These descending LC/SC neurons have been confirmed to be involved in modulation of nociceptive transmission in the spinal cord. Activation of the LC/ SC region either electrically or chemically can produce profound antinociception [6] and can inhibit nociceptive activity of spinal dorsal horn neurons [7,17] and trigeminal subnucleus caudalis neurons [19]. Thus, the coeruleospinal modulation system appears to play a significant role in spinal nociceptive processing.…”

Section: Introductionmentioning

confidence: 97%

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A possible synaptic configuration underlying coeruleospinal inhibition of visceral nociceptive transmission in the rat

et al. 2011

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A synaptic arrangement underlying descending inhibition from the locus coeruleus/subcoeruleus (LC/SC) on visceral nociceptive transmission in the spinal cord was investigated in the anesthetized rat. Extracellular recordings were made from the L(6)-S(2) segmental level using a carbon filament glass microelectrode (4-6 MΩ). Colorectal distention (CRD) was produced by inflating a balloon inside the descending colon and rectum. All neurons tested responded to both CRD and to cutaneous pinch (a force of 613 g/mm(2)), indicating that nociceptive signals from visceral organs and nociceptive signals from the cutaneous receptive field converge on a single neuron. These neurons were divided into two groups based on their response to CRD: short latency-abrupt and short latency-sustained neurons. Electrical stimulation of the LC/SC (30 or 50 μA, 100 Hz, 0.1 ms pulses) inhibited both CRD-evoked and cutaneous pinch-evoked responses in short latency-abrupt and short latency-sustained neurons. When graded CRD (20, 40, 60, and 80 mmHg) was delivered, LC/SC stimulation produced a reduction in slope of the linear CRD intensity-response magnitude curve without a change in the response threshold in both short latency-abrupt (n = 42) and short latency-sustained neurons (n = 11). This result suggests that coeruleospinal inhibition of visceral nociceptive transmission is due to a synaptic configuration in which inhibitory and excitatory terminals are in close spatial proximity, including presynaptic inhibition.

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Section: Discussioncontrasting

confidence: 57%

Section: Lc/sc Stimulationmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 97%

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A possible synaptic configuration underlying coeruleospinal inhibition of visceral nociceptive transmission in the rat

et al. 2011

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“…Some of the densest concentrations of terminals from the MVe and of cells projecting to the MVe are in the subcoeruleus nuclei that modulate nociception as well as control of micturition and sexual function (Aston-Jones et al, 1991;Sugaya et al, 1998;Tsuruoka et al, 2003). Dorsally, Barrington's nucleus, also implicated in micturition control (Valentino et al, 1994), contained moderately dense terminals and labeled cells following large or small tracer injections into the MVe.…”

Section: Connections To Sleep and Arousal Systemsmentioning

confidence: 97%

“…Pretectal nuclei are retinorecipient (Morin and Blanchard, 1997) and the olivary pretectal nucleus in particular is part of the pupillary constriction circuit (Klooster et al, 1995;Chan et al, 1995;Clarke et al, 2003). The anterior pretectal nucleus is implicated in nociception processing (Foster et al, 1989;Prado and Faganello, 2000;Villarreal et al, 2004), a feature consistent with the MVe connections with the subcoeruleus area that also participates in nociception (Tsuruoka et al, 2003).…”

Section: Visuomotor System Connectionsmentioning

confidence: 97%

Medial vestibular connections with the hypocretin (orexin) system

2005

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The mammalian medial vestibular nucleus (MVe) receives input from all vestibular endorgans and provides extensive projections to the central nervous system. Recent studies have demonstrated projections from the MVe to the circadian rhythm system. In addition, there are known projections from the MVe to regions considered to be involved in sleep and arousal. In this study, afferent and efferent subcortical connectivity of the medial vestibular nucleus of the golden hamster (Mesocricetus auratus) was evaluated using cholera toxin subunit-B (retrograde), Phaseolus vulgaris leucoagglutinin (anterograde), and pseudorabies virus (transneuronal retrograde) tract-tracing techniques. The results demonstrate MVe connections with regions mediating visuomotor and postural control, as previously observed in other mammals. The data also identify extensive projections from the MVe to regions mediating arousal and sleep-related functions, most of which receive immunohistochemically identified projections from the lateral hypothalamic hypocretin (orexin) neurons. These include the locus coeruleus, dorsal and pedunculopontine tegmental nuclei, dorsal raphe, and lateral preoptic area. The MVe itself receives a projection from hypocretin cells. CTB tracing demonstrated reciprocal connections between the MVe and most brain areas receiving MVe efferents. Virus tracing confirmed and extended the MVe afferent connections identified with CTB and additionally demonstrated transneuronal connectivity with the suprachiasmatic nucleus and the medial habenular nucleus. These anatomical data indicate that the vestibular system has access to a broad array of neural functions not typically associated with visuomotor, balance, or equilibrium, and that the MVe is likely to receive information from many of the same regions to which it projects.

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Lentivectors are efficient tools to manipulate the dendritic cell cytoskeleton

Metelo¹,

Ward²,

Thrasher

et al. 2011

Cytoskeleton

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Dendritic cells (DC) are key cells of the innate immune system required to prime adaptive immunity. Central DC functions including antigen uptake and presentation and DC migration are critically dependent on dynamic cytoskeletal reorganisation, the regulation of which remains poorly understood. Cytoskeletal studies are complicated by the fact that DC cytoarchitecture is altered considerably by maturation stimuli, including many tools employed for biological manipulation. Lentiviral vectors, capable of transducing non-dividing cells such as DC, hold promise both for experimental and therapeutic manipulation of DC gene and protein expression but controversy remains about their effect on DC maturation. Here, we have examined the potential of lentiviral vectors as tools for gene delivery to monocyte derived human DC with preservation of immature DC cytoskeletal structure and function. We show that vesicular stomatitis virus G glycoprotein (VSVG)-pseudotyped lentivectors are most efficient at transducing immature DC and their precursor monocytes. Even at high multiplicities of infection transduced DC retained an immature cytoskeletal phenotype, with no significant alteration of migration, antigen uptake or T-cell stimulation capacities. Furthermore, lentivectors did not alter subsequent functional maturation of the DC cytoskeleton in response to lipopolysaccharide exposure. Together our data show that VSVG-psudotyped lentiviral vectors are an effective tool for gene manipulation in human DC with preservation of functional immaturity and plasticity, making them ideal for studies of the DC cytoskeleton.

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Coeruleotrigeminal inhibition of nociceptive processing in the rat trigeminal subnucleus caudalis

Cited by 32 publications

References 39 publications

A possible synaptic configuration underlying coeruleospinal inhibition of visceral nociceptive transmission in the rat

A possible synaptic configuration underlying coeruleospinal inhibition of visceral nociceptive transmission in the rat

Medial vestibular connections with the hypocretin (orexin) system

Lentivectors are efficient tools to manipulate the dendritic cell cytoskeleton

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