Coenzyme Q10 Efficacy Test for Human Skin Equivalents Using a Pumpless Skin-On-A-Chip System

Kim, Jisue; Kim, Kyung‐Hee; Sung, Gun Yong

doi:10.3390/ijms21228475

Cited by 28 publications

(25 citation statements)

References 20 publications

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“…Three type I collagen studies have confirmed that rat-tail collagen is the most appropriate scaffold [ 32 ] and that pumpless SOC produces anticancer-drug-induced side effects on the skin [ 33 ] and anti-aging effects. We have reported the effects of curcuma longa leaf extract (CLLE) [ 34 ] and CoQ10 drugs [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of α-Lipoic Acid on the Development of Human Skin Equivalents Using a Pumpless Skin-on-a-Chip Model

Kim

et al. 2021

IJMS

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Owing to the prohibition of cosmetic animal testing, various attempts have recently been made using skin-on-a-chip (SOC) technology as a replacement for animal testing. Previously, we reported the development of a pumpless SOC capable of drug testing with a simple drive using the principle that the medium flows along the channel by gravity when the chip is tilted using a microfluidic channel. In this study, using pumpless SOC, instead of drug testing at the single-cell level, we evaluated the efficacy of α-lipoic acid (ALA), which is known as an anti-aging substance in skin equivalents, for skin tissue and epidermal structure formation. The expression of proteins and changes in genotyping were compared and evaluated. Hematoxylin and eosin staining for histological analysis showed a difference in the activity of fibroblasts in the dermis layer with respect to the presence or absence of ALA. We observed that the epidermis layer became increasingly prominent as the culture period was extended by treatment with 10 μM ALA. The expression of epidermal structural proteins of filaggrin, involucrin, keratin 10, and collagen IV increased because of the effect of ALA. Changes in the epidermis layer were noticeable after the ALA treatment. As a result of aging, damage to the skin-barrier function and structural integrity is reduced, indicating that ALA has an anti-aging effect. We performed a gene analysis of filaggrin, involucrin, keratin 10, integrin, and collagen I genes in ALA-treated human skin equivalents, which indicated an increase in filaggrin gene expression after ALA treatment. These results indicate that pumpless SOC can be used as an in vitro skin model similar to human skin, protein and gene expression can be analyzed, and it can be used for functional drug tests of cosmetic materials in the future. This technology is expected to contribute to the development of skin disease models.

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Section: Introductionmentioning

confidence: 99%

Effect of α-Lipoic Acid on the Development of Human Skin Equivalents Using a Pumpless Skin-on-a-Chip Model

Kim

et al. 2021

IJMS

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“…When the skin is damaged, it activates cytokines’ expression, causing inflammation in the damaged area and fighting against the potential intruder pathogens. Angiogenesis and the pro-angiogenic factors like PDGF (platelet-derived growth factor), VEGF (vascular endothelial growth factor), and TGF-β (transforming growth factor β) can help in the regeneration of the tissue by stimulating the proliferation, migration, and tube formation of endothelial cells [ 87 , 88 , 89 , 90 ]. Studies have shown similarities in the skin defense reaction and repairing mechanism in high UV exposure and skin surface damage, including inflammation, increased cell migration, and proliferation [ 83 , 91 ].…”

Section: Utilization Of Skin-on-a-chip Systemsmentioning

confidence: 99%

“…As different warning signs appear in a tissue, cytokine expression and release increases for activating the defense of the body. This signal cascade can be started by tissue damage, pathogens, and foreign bodies, but in the case of the skin, it can also be the consequence of high exposure to UV light [ 83 , 88 , 89 , 90 ].…”

Section: Utilization Of Skin-on-a-chip Systemsmentioning

confidence: 99%

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Development of Skin-On-A-Chip Platforms for Different Utilizations: Factors to Be Considered

et al. 2021

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There is increasing interest in miniaturized technologies in diagnostics, therapeutic testing, and biomedicinal fundamental research. The same is true for the dermal studies in topical drug development, dermatological disease pathology testing, and cosmetic science. This review aims to collect the recent scientific literature and knowledge about the application of skin-on-a-chip technology in drug diffusion studies, in pharmacological and toxicological experiments, in wound healing, and in fields of cosmetic science (ageing or repair). The basic mathematical models are also presented in the article to predict physical phenomena, such as fluid movement, drug diffusion, and heat transfer taking place across the dermal layers in the chip using Computational Fluid Dynamics techniques. Soon, it can be envisioned that animal studies might be at least in part replaced with skin-on-a-chip technology leading to more reliable results close to study on humans. The new technology is a cost-effective alternative to traditional methods used in research institutes, university labs, and industry. With this article, the authors would like to call attention to a new investigational family of platforms to refresh the researchers’ theranostics and preclinical, experimental toolbox.

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“…Our research confirmed that providing media to a pumpless skin-on-a-chip system with gravity flow using 3D skin equivalents cultured on transwell inserts and microfluidic chips provided sufficient nutrients for cells to multiply and differentiate [ 24 ]. Furthermore, human skin equivalent models that use pumpless skin-on-a-chip systems with gravity flow are used as part of drug screening platforms to evaluate drug efficacy [ 24 , 30 , 31 , 32 , 33 , 34 ]. A previous study also confirmed that the thickness of the epidermal layer was increased by mechanical stretching [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Development of an Aged Full-Thickness Skin Model Using Flexible Skin-on-a-Chip Subjected to Mechanical Stimulus Reflecting the Circadian Rhythm

Jeong

Kim

Jeon

et al. 2021

IJMS

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The skin is subject to both intrinsic aging caused by metabolic processes in the body and extrinsic aging caused by exposure to environmental factors. Intrinsic aging is an important obstacle to in vitro experimentation as its long-term progression is difficult to replicate. Here, we accelerated aging of a full-thickness skin equivalent by applying periodic mechanical stimulation, replicating the circadian rhythm for 28 days. This aging skin model was developed by culturing a full-thickness, three-dimensional skin equivalent with human fibroblasts and keratinocytes to produce flexible skin-on-a-chip. Accelerated aging associated with periodic compressive stress was evidenced by reductions in the epidermal layer thickness, contraction rate, and secretion of Myb. Increases in β-galactosidase gene expression and secretion of reactive oxygen species and transforming growth factor-β1 were also observed. This in vitro aging skin model is expected to greatly accelerate drug development for skin diseases and cosmetics that cannot be tested on animals.

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Coenzyme Q10 Efficacy Test for Human Skin Equivalents Using a Pumpless Skin-On-A-Chip System

Cited by 28 publications

References 20 publications

Effect of α-Lipoic Acid on the Development of Human Skin Equivalents Using a Pumpless Skin-on-a-Chip Model

Effect of α-Lipoic Acid on the Development of Human Skin Equivalents Using a Pumpless Skin-on-a-Chip Model

Development of Skin-On-A-Chip Platforms for Different Utilizations: Factors to Be Considered

Development of an Aged Full-Thickness Skin Model Using Flexible Skin-on-a-Chip Subjected to Mechanical Stimulus Reflecting the Circadian Rhythm

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