Coelenterazine is a superoxide anion-sensitive chemiluminescent probe: Its usefulness in the assay of respiratory burst in neutrophils

Lucas, Miguel; Solano, Francisca

doi:10.1016/0003-2697(92)90366-f

Cited by 69 publications

(43 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In response to ROS it generates a signal similar to that expected of an increase in Ca 2+ (Lucas and Solano 1992;Plieth 2005;Molecular Probes 2006). Therefore, experiments using this approach require careful controls for possibly confounding effects of ROS production.…”

Section: Ca 2+ Signaling In the Touch And Gravity Responsementioning

confidence: 88%

Touch Sensing and Thigmotropism

Monshausen¹,

Swanson²,

Gilroy³

2007

Plant Tropisms

View full text Add to dashboard Cite

Section: Ca 2+ Signaling In the Touch And Gravity Responsementioning

confidence: 88%

Touch Sensing and Thigmotropism

Monshausen¹,

Swanson²,

Gilroy³

2007

Plant Tropisms

View full text Add to dashboard Cite

“…75,76 Although instability, tissue metabolism, the sometimes broad reactivity of spin traps, and the cost of ESR spectrometers can be problematic, when combined with parallel strategies for detecting specific reactive species, ESR has proven to be a useful and revealing free radical detection strategy. [77][78][79] Lucigenin and LumH 2 and the light they can produce have long been studied in the hope that understanding these chemiluminescences would lead to insight into the mechanism of bioluminescence. Thus, Gleu and Petsch 80 knew in 1935 that oxidants such as hypochlorite or ferricyanide would elicit LumH 2 luminescence, provided that O 2 was also present, but these oxidants did not cause Luc 2ϩ to emit light.…”

Section: Electron Spin Resonance and Spin Trappingmentioning

confidence: 99%

Methods of Detection of Vascular Reactive Species

Tarpey

Fridovich²

2001

Circulation Research

481

106

View full text Add to dashboard Cite

Abstract-The evanescent nature of reactive oxygen and nitrogen species, the multiple cellular mechanisms evolved to maintain these substances at low (submicromolar) concentrations within the vascular system, and the often multifaceted nature of their reactivities have made measurement of these compounds within the vasculature problematic. This review attempts to provide a critical description of some of the most common approaches to quantification of nitric oxide, superoxide, hydrogen peroxide, and peroxynitrite, with attention to key issues that may influence the utility of a particular assay when adapted for use in vascular cells and tissues.

show abstract

“…Oxidation of free thiols on membranes by peroxynitrite was measured by the DTNB assay as above. Superoxide dismutase (SOD)-sensitive superoxide production was measured using the reaction of superoxide with coelenterazine to produce a chemiluminescent product (62). This probe is less susceptible to interaction with the respiratory chain than acetylated cytochrome c. Membranes (1 mg of protein/ml) were incubated in KPi buffer with various additions for 10 min, then washed twice and resuspended at 1 mg of protein/ml in KP i buffer.…”

Section: Methodsmentioning

confidence: 99%

Persistent S-Nitrosation of Complex I and Other Mitochondrial Membrane Proteins by S-Nitrosothiols but Not Nitric Oxide or Peroxynitrite

Dahm

Moore

Murphy

2006

Journal of Biological Chemistry

186

142

View full text Add to dashboard Cite

S-Nitrosation of mitochondrial proteins has been proposed to contribute to the pathophysiological interactions of nitric oxide (NO) and its derivatives with mitochondria but has not been shown directly. Furthermore, little is known about the mechanism of formation or the fate of these putative S-nitrosothiols. Here we have determined whether mitochondrial membrane protein thiols can be S-nitrosated on exposure to free NO from 3,3-bis(aminoethyl)-1-hydroxy-2-oxo-1-triazene (DETA-NONOate) by interaction with S-nitrosoglutathione or S-nitroso-N-acetylpenicillamine (SNAP) and by the NO derivative peroxynitrite. S-Nitrosation of protein thiols was measured directly by chemiluminescence detection. S-Nitrosoglutathione and S-nitroso-N-acetylpenicillamine led to extensive protein thiol oxidation, with about 30% of the modified protein thiols persistently S-nitrosated. In contrast, there was no protein thiol oxidation or S-nitrosation on exposure to 3,3-bis (aminoethyl)-1-hydroxy-2-oxo-1-triazene. Peroxynitrite extensively oxidized protein thiols but produced negligible amounts of S-nitrosothiols. Therefore, mitochondrial membrane protein thiols are S-nitrosated by preformed S-nitrosothiols but not by NO or by peroxynitrite. These S-nitrosated protein thiols were readily reduced by glutathione, so S-nitrosation will only persist when the mitochondrial glutathione pool is oxidized. Respiratory chain complex I was S-nitrosated by S-nitrosothiols, consistent with it being an important target for S-nitrosation during nitrosative stress. The S-nitrosation of complex I correlated with a significant loss of activity that was reversed by thiol reductants. S-Nitrosation was also associated with increased superoxide production from complex I. These findings point to a significant role for complex I S-nitrosation and consequent dysfunction during nitrosative stress in disorders such as Parkinson disease and sepsis.

show abstract

Coelenterazine is a superoxide anion-sensitive chemiluminescent probe: Its usefulness in the assay of respiratory burst in neutrophils

Cited by 69 publications

References 16 publications

Touch Sensing and Thigmotropism

Touch Sensing and Thigmotropism

Methods of Detection of Vascular Reactive Species

Persistent S-Nitrosation of Complex I and Other Mitochondrial Membrane Proteins by S-Nitrosothiols but Not Nitric Oxide or Peroxynitrite

Contact Info

Product

Resources

About