Coculturing denuded oocytes during the in vitro maturation of bovine cumulus oocyte complexes exerts a synergistic effect on embryo development

Oocyte maturation with royal jelly increases embryo development and reduces apoptosis in goats

“…The synergistic effect of royal jelly on embryo development could be attributed to improving the IVM microenvironment (Choi et al, 2013), in addition to increasing nuclear and cytoplasmic maturation of oocytes (Dey et al, 2012). Interestingly, it seems that the royal jelly antioxidative potentiality could also be sustained throughout embryo cleavage until blastocyst formation, as observed in the current study and previous reports when various antioxidant supplements were used during IVM (Ali et al, 2003;Rodríguez-González et al, 2003;Kwak et al, 2012;Do et al, 2015;Fakruzzaman et al, 2015;Mishra et al, 2016).…”

“…There are different ways to improve the oocyte microenvironment during the course of the maturation process. Co-culture of intact COCs with a specific ratio of denuded oocytes has improved nuclear, cytoplasmic maturation (Dey et al, 2012;Souza-Fabjan et al, 2016) and the rate of blastocyst formation, in addition to quality (Choi et al, 2013). Antioxidants such as peroxiredoxin II (Fakruzzaman et al, 2015), melatonin (Do et al, 2015), resveratrol (Kwak et al, 2012), l-carnitine 131 (Mishra et al, 2016), cysteine (Ali et al, 2003), and cysteamine (Rodríguez-González et al, 2003) have been supplemented to IVM media to improve developmental competence of pre-implantation blastocysts of different mammalian species.…”

Effect of peroxiredoxin II on the quality and mitochondrial activity of pre-implantation bovine embryos

“…In the present study, although mechanism by which this occurred was not fully investigated, it may be that PRDX II treatment as antioxidant enzyme maintains the mitochondrial function by removing ROS, and produces "healthy" matured oocytes, result in improved blastocyst quality. Therefore, it is speculated from our results and those reported previously (Albuz et al, 2010;Deb et al, 2012;Dey et al, 2012;Choi et al, 2013) that improving IVM condition could enable the embryo to bypass the post-fertilization in vitro conditions. Although our data partially support this idea, further investigations using global profiling tool like microarray could help more to understand the molecular mechanisms involved in pronounced improving embryo quality.…”

Interaction of donor age, parity and repeated recovery of cumulus–oocyte complexes by ovum pick-up on in vitro embryo production and viability after transfer

“…Accordingly, grade 1þ 2 COCs were considered good quality while grade 3 þ 4 were considered low quality ones. All COCs retrieved of each donor were washed twice in maturation medium supplemented with 10% (v/v) FBS, 1 mg/mL of estradiol-17β, 10 mg/mL of FSH, 0.6 mM of cystein and 0.2 mM of sodium pyruvate, 50 mg/mL gentamycin sulphate and transferred in 700 mL of IVM medium at 38.5°C in a humidified atmosphere of 5% CO 2 in air for 23-24 h, and cultured as described previously (Dey et al, 2012).…”