Coculture of endothelial progenitor cells and mesenchymal stem cells enhanced their proliferation and angiogenesis through <scp>PDGF</scp> and Notch signaling

Tang, Liang; Zhu, Lei; Gao, Weilun; Gong, Ming; Ren, Jianhua; Yao, Hui; Wang, Kun; Shi, Dongquan

doi:10.1002/2211-5463.12317

Cited by 58 publications

(48 citation statements)

References 40 publications

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“…High VWF mRNA levels in tumors may be an early sign of activation of the endothelium (43). PDGFR serves a key role in regulating the formation and function of blood vessels (44). These gene expression results reflect the findings of the present functional assays.…”

Section: Discussionsupporting

confidence: 83%

Tumor‑type‑dependent effects on the angiogenic abilities of endothelial cells in an in vitro rat cell model

Al‐Abboodi

Weber

et al. 2019

Oncol Rep

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Adequate vascularization is pivotal for tumor progression and metastasis. Tumor angiogenesis is based on a sequence of interactions between the tumor and surrounding cells and the extracellular matrix. It is widely known that a tumor can influence and control its surroundings to create favorable conditions for further growth. To investigate the influence of various tumor types on endothelial cells (ECs), an in vitro rat cell model was used and rat liver EC52 cells were co-cultured with conditioned medium derived from breast cancer MCR86, osteosarcoma ROS-1, colon cancer CC531 and rhabdomyosarcoma R1H cell lines. In a distinct tumor-type-dependent manner, the EC52 cells exhibited changes in their function and gene expression. In all functional cell culture assays (proliferation, migration, transmigration, invasion and tube formation) the breast cancer cells exerted a significant effect on the angiogenic abilities of the ECs. When comparing the various tumor cell types, only the breast and colon cancer cells led to a significant stimulation of the EC migration and invasion. Proliferation, migration, invasion and tube formation were not or only hardly influenced by the osteosarcoma or rhabdomyosarcoma cells. Similarly, the breast and colon cancer cells exhibited the strongest influence on the upregulation of EC angiogenic genes, including the ones encoding vascular endothelial growth factor A, platelet and endothelial cell adhesion molecule 1, fibroblast growth factor 2, Von Willebrand factor, C-X-C motif chemokine ligand 12 and tyrosine kinase with immunoglobulin-like and EGF-like domains 1. Therefore, it is hypothesized that tumor cells enhance the angiogenic properties of ECs, including proliferation, migration, invasion and tube formation in a tumor-type-dependent manner. This is likely based on the upregulation of pro-angiogenic genes in ECs induced by varying cytokine secretion signatures of tumor cells.

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Section: Discussionsupporting

confidence: 83%

Tumor‑type‑dependent effects on the angiogenic abilities of endothelial cells in an in vitro rat cell model

Al‐Abboodi

Weber

et al. 2019

Oncol Rep

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“…A study shows that BMSCs differentiate into endotheliocytes and pericytes for angiogenesis after they are injected into uterine scar tissue in the rat [ 28 ]. Coculturing endothelial progenitor cells and BMSCs enhances their proliferation and angiogenesis through platelet-derived growth factor (PDGF) and translocation-associated (Notch) signaling [ 29 ]. In addition, BMSC-derived angiogenin has a positive effect on regulating angiogenesis in grafted human ovarian tissue [ 30 ].…”

Section: Angiogenesismentioning

confidence: 99%

The therapeutic potential of bone marrow mesenchymal stem cells in premature ovarian failure

et al. 2018

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With the development of regenerative medicine, a variety of mesenchymal stem cells (MSCs) are increasingly considered for the treatment of premature ovarian failure (POF). Reportedly, bone marrow-derived MSCs (BMSCs) improve the ovarian reserve, which mainly depends on homing and paracrine activities. Furthermore, paracrine factors secreted by these stem cells play an important role in ovarian recovery. Relevant studies indicate that BMSC transplantation has some positive effects on the treatment of POF in animals, but BMSCs are not widely applied in clinical therapy. Clinical trials are ongoing despite the fact that several patients experiencing BMSC transplantation recover their normal menstrual cycles and even give birth to babies. In this review, we discuss the possible therapeutic mechanisms of BMSCs for POF, migration, antiapoptosis, antifibrosis, angiogenesis, anti-inflammation, immunoregulation, and oxidative stress, which provide the theoretical basis for further study and clinical therapy.

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“…The Notch signaling pathway serves a vital role in angiogenesis, and changes in the pathway lead to the abnormal development of blood vessels (13). Previous studies have reported that the Notch signaling pathway serves an important role in stem cell proliferation and angiogenesis (14).…”

Section: Introductionmentioning

confidence: 99%

Notch‑1 inhibition reduces proliferation and promotes osteogenic differentiation of bone marrow mesenchymal stem cells

Zou

2019

Exp Ther Med

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Low differentiation and high proliferation rates are critical factors affecting bone marrow mesenchymal stem cell (BMSC) tumorigenesis. The present study aimed to investigate the role of the Notch signaling pathway in BMSC proliferation and osteogenic differentiation. Mouse BMSCs were divided into control, vector, Notch1-small interfering (si)RNA, γ-secretase inhibitor, and Notch1-siRNA + γ-secretase inhibitor groups. The siRNA-Notch1, γ-secretase inhibitor, and Notch1-siRNA + γ-secretase inhibitor groups were treated with Notch1 siRNA and/or γ-secretase inhibitor. Following treatment, cell proliferation was evaluated using a Cell Counting Kit-8. Tumor-related factors, including transforming growth factor (TGF)-β1, c-Myc and p53, were detected by reverse transcription-quantitative polymerase chain reaction and western blot analyses. BMSC osteogenic differentiation was induced and the cells were stained with alizarin red at 14 and 21 days. Alkaline phosphatase (AKP) activity was also evaluated. The siRNA-Notch1 and γ-secretase inhibitor both reduced BMSC proliferation and the expression of TGF-β1 and c-Myc and increased the expression of p53. Following the induction of osteogenesis and staining with alizarin red, the level of AKP was significantly higher in cells in the siRNA-Notch1 and γ-secretase inhibitor groups compared with that in the control group. It was found that Notch1 inhibition reduced proliferation and promoted the osteogenic differentiation of BMSCs.

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Coculture of endothelial progenitor cells and mesenchymal stem cells enhanced their proliferation and angiogenesis through PDGF and Notch signaling

Cited by 58 publications

References 40 publications

Tumor‑type‑dependent effects on the angiogenic abilities of endothelial cells in an in vitro rat cell model

Tumor‑type‑dependent effects on the angiogenic abilities of endothelial cells in an in vitro rat cell model

The therapeutic potential of bone marrow mesenchymal stem cells in premature ovarian failure

Notch‑1 inhibition reduces proliferation and promotes osteogenic differentiation of bone marrow mesenchymal stem cells

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