Coculture of Bladder Urothelial and Smooth Muscle Cells on Small Intestinal Submucosa: Potential Applications for Tissue Engineering Technology

Zhang, Yuanyuan; Kropp, Bradley P.; Moore, P. J.; Cowan, Rick; Furness, Peter D.; Kolligian, Mark E.; Frey, Peter; Cheng, Earl Y.

doi:10.1016/s0022-5347(05)67220-5

Cited by 131 publications

(69 citation statements)

References 24 publications

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“…Individual cell suspensions were washed twice with Hank's balanced salt solution, resuspended and plated on cell culture flasks. Primary cultures of smooth muscle cells were cultured in modified F12 media supplemented with 10% FBS and routinely passaged as described by Baskin et al [3] . All cultures were incubated in 5% carbon dioxide at 37 ℃ and fed with fresh media every 2 days.…”

Section: Cell Culturementioning

confidence: 99%

Biocompatibility of compounds of extracellular matrix and thermally reversible hydrogel

Yao

Yang

Zhang

et al. 2007

J. Wuhan Univ. Technol.

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A new scaffold material composed of extracellular matrix (ECM) and thermal sensitive hydrogel (HG), and evaluated its biocompatibility were investigated. We cultured bladder smooth muscle cells with this compound material, and then observed with phase contrast microscopy and scanning electron microscope (SEM) to assess the cell growth and morphology. The cell adhesion and proliferation were detected with MTT assay and cell count. Results show the ECM/HG compounds appeared as a net-like and red-stained construction with enough meshes and without any cellular fragments. 6 h after implantation, cells were observed adhere on the compounds and extend spurious along the fibers 12 h later. Under SEM even some ECM was observed to be secreted. MTT assay shows there was obvious statistic difference among 3 groups (P<0.05). ECM/HG compound materials show a good biocompatibility, which confirms that it would be an ideal tissue engineering scaffolds.

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Section: Cell Culturementioning

confidence: 99%

Biocompatibility of compounds of extracellular matrix and thermally reversible hydrogel

Yao

Yang

Zhang

et al. 2007

J. Wuhan Univ. Technol.

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“…Zunächst stand die Entwicklung geeigneter Matrixstrukturen im Vordergrund, die später durch Besiedelung mit körpereigenen Zellen eine komplette Rekonstruktion von Harnblase, Corpora cavernosa sowie Urethra erlaubten [24,45]. Die Problematik dieser ausdifferenzierten somatischen Zellen besteht einerseits in ihrer schlechten Kultivierbarkeit und andererseits in ihrem sehr eingeschränkten Proliferationspotenzial.…”

Section: Embryonale Stammzellenunclassified

Embryonale und adulte Stammzellen f�r Tissue Engineering in der Urologie

Bartsch

Frimberger

2004

Urologe [A]

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Stem cells are considered a possible alternative treatment for degenerative diseases. Parkinson's disease and diabetes mellitus may become curable using stem cells. The term "stem cell" is used very broadly, although it has been well defined. Undifferentiated cells (stem cells and progenitor cells) are isolated from prenatal and postnatal tissues. This review provides an overview of embryonic and adult stem cells and describes potential applications in the field of tissue engineering in urology.

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“…In vivo implantation of SIS and other collagen matrices derived from bladder submucosa has demonstrated the ability to promote tissue-specific regeneration of functional bladder segments [9][10][11][12][13].…”

Section: Unseeded Technologymentioning

confidence: 99%

“…SIS is a unique substrate that provides an in vitro environment for bladder urothelial and smooth muscle cells to interact with each other and with the surrounding matrix in a manner that is similar to what is observed in vivo during the early events in bladder regeneration. Seeding of bladder cells on SIS with different co-culture techniques and subsequent placement of the cell-matrix construct into the host for continued regeneration may enhance our abilities to provide a patient with new bladder tissue for replacement therapy [10]. Other investigations into cell-seeded SIS demonstrated multilayered bladder regeneration in a nude mouse model [19].…”

Section: Cell-seeded Technologymentioning

confidence: 99%

Tissue engineering in urology: Where are we going?

2003

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Tissue engineering in urology is a broad term used to describe the development of alternative tissue sources for diseased or dysfunctional native urologic tissue. This article reviews the recently published techniques involving synthetic and natural biodegradable matrices alone, known as "unseeded" scaffolds, and the latest data on "seeded" scaffolds, which are impregnated with cultured cells from urologic organs. Recent discoveries in reporter gene labeling of urologic tissue are discussed as a new method to identify and track the fates of these transplanted cells in vivo. This article also investigates how these bioengineering techniques are applied to synthetic and natural scaffolds, such as polyglycolic acid and porcine small intestine submucosa, to increase bladder capacity, repair urethral strictures, and replace corporal plaques in Peyronie's disease. Furthermore, recently published reports that these materials have been seeded with chondrocytes to create corporal rods for penile prostheses and stents for ureteral and urethral stricture disease are discussed. With these latest developments as a foundation, the future directions of tissue engineering in urology are presented.

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Coculture of Bladder Urothelial and Smooth Muscle Cells on Small Intestinal Submucosa: Potential Applications for Tissue Engineering Technology

Cited by 131 publications

References 24 publications

Biocompatibility of compounds of extracellular matrix and thermally reversible hydrogel

Biocompatibility of compounds of extracellular matrix and thermally reversible hydrogel

Embryonale und adulte Stammzellen f�r Tissue Engineering in der Urologie

Tissue engineering in urology: Where are we going?

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