Introduction: Early detection of viral gastroenteritis is important because it causes hospitalizations in children and administration of antibiotics unnecessarily. Rotavirus and adenovirus are the most common viral pathogens in childhood gastroenteritis. The aim of this study was to investigate the rate of rotavirus and adenovirus in stool samples sent from children with acute gastroenteritis. Methods: The results of stool samples sent to the Tokat Gaziosmanpasa University Medical Faculty Research and Practice Hospital Microbiology Laboratory for the investigation of rotavirus and adenovirus antigens from children diagnosed with gastroenteritis between January 2017 and January 2018 were evaluated retrospectively. Rotavirus and adenovirus 40/41 antigens were detected by immunochromatography. Results: There were 1635 and 1608 patients' samples analyzed for rotavirus and adenovirus antigens, respectively. Rotavirus was detected in 133 (8.1%) patients, adenovirus in 30 (1.8%) and both agents in seven (0.4%) patients. Rotavirus antigen positivity was found to be significantly higher (p<0.05) in the patients aged between 13-24 months compared to other age groups. There was no significant difference between age groups in terms of adenovirus positivity (p>0.05). The majority of rotavirus gastroenteritis cases were seen in the winter (43.6%) and it was statistically significant (p<0.05). Discussion and Conclusion: Rapid antigen testing for viral gastroenteritis agents contributes to the planning of gastroenteritis treatment and decreases unnecessary antibiotic usage in children. It has been also concluded that it is important to consider the possibility of rotavirus gastroenteritis in children older than five years since 13.5% of patients were in this group.