Cockroach Giant Interneurones Stained by Cobalt-Backfilling of Dissected Axons

Harrow, I D; Hue, Bernard; Pelhate, M.; Sattelle, D. B.

doi:10.1242/jeb.84.1.341

Cited by 49 publications

(4 citation statements)

References 9 publications

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“…Although axonal position noted during dissection proved a fairly reliable indicator that the cell under test was GI 2, this could only be confirmed on completion of a pharmacological experiment by backfilling the isolated axon with cobaltous chloride, ^i n g a procedure described earlier (Harrow et al 1980).…”

Section: Identification Ofgi2mentioning

confidence: 99%

“…2). The single axon backfill procedure (Harrow et al 1980) allowed identification of GI 2 upon completion of electrophysiological experiments. Each giant interneurone has a unique morphology in the terminal abdominal ganglion (Daley, Vardi, Appignani & Camhi, 1981).…”

Section: Actions Of A-bungarvtoxin On the Axon Ofgi2mentioning

confidence: 99%

“…It is also possible to record synaptic potentials for long periods from a single giant interneurone by the oil-gap, single-fibre technique (Pichon & Callec, 1970), using the dissected axon of one of the giant interneurones. After pharmacological experiments the giant axon can be cobalt backfilled for identification (Harrow, Hue, Pelhate & Sattelle, 1980).…”

Section: Introductionmentioning

confidence: 99%

“…Fig.3. Camera lucida drawings of unintenaified cobalt fills of GI 2 prepared by the axonal backfill method(Harrow, Hue, Pelhate & Sattelle, 1980). (A) Desheathed preparation of the sixth abdominal ganglion in which cobalt filling began after the compound EPSP recorded from the interneurone was blocked by a-bungarotoxin (1-0 X 10"'M).…”

mentioning

confidence: 99%

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α-Bungarotoxin Blocks Excitatory Synaptic Transmission Between Cercal Sensory Neurones and Giant Interneurone 2 of the Cockroach,Periplaneta Americana

Sattelle

Harrow

Hue

et al. 1983

Journal of Experimental Biology

109

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1. Autoradiographic localization of an 125I-α-bungarotoxin binding component revealed that specific binding was distributed mainly in the neuropile and to some extent in the periphery of the terminal abdominal ganglion of the cockroach, Periplaneta americana (L.). 2. Action potentials recorded from the axon of GI2 under current-clamp conditions were not affected by exposure to 1.0×10−5M α-bungarotoxin. 3. Excitatory postsynaptic potentials recorded from GI2, evoked by stimulation of cereal sensory neurones, were sensitive to block by relatively low concentrations (1.0×10−9-1.0×10−7 M) of α-bungarotoxin. The time-course for blockade was found to depend on toxin concentration and frequency of afferent stimulation. 4. Excitatory postsynaptic potentials recorded from GI 2 were not affected by exposure to 1.0×10−6M quinuclidinyl benzilate. 5. We conclude that at least a portion of the 125I-α-bungarotoxin binding component represents cholinergic receptors which have a postsynaptic function at synapses between cereal sensory neurones and GI 2.

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Section: Identification Ofgi2mentioning

confidence: 99%

Section: Actions Of A-bungarvtoxin On the Axon Ofgi2mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

α-Bungarotoxin Blocks Excitatory Synaptic Transmission Between Cercal Sensory Neurones and Giant Interneurone 2 of the Cockroach,Periplaneta Americana

Sattelle

Harrow

Hue

et al. 1983

Journal of Experimental Biology

109

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Acetylcholine Receptors of Identified Insect Neurons

Harrow

David

Sattelle

1982

Ciba Foundation Symposium 88 ‐ Neuropharmacology of Insects

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Functional assay for GABA receptor subtypes of a cockroach giant interneuron

Hue¹

1991

Arch Insect Biochem Physiol

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gamma-Aminobutyric acid (GABA) receptors were examined in the cockroach central nervous system (CNS) using the single fiber-oil gap method applied to an identified giant interneuron. Short-lasting pressure application of 10 mM GABA developed a multiphasic response composed of a fast hyperpolarization followed by a transient depolarizing component and a stable hyperpolarization. This triphasic characteristic shape of the response was modified according to the dose of GABA injected or bath-applied and to the precise localization of the injection within the dendritic area. The transient depolarizing phase showed a negative reversal potential of -70 mV. Both hyperpolarizing phases reversed at a more negative level ranging to -80 mV. A positive shift of these values was caused by a decrease in external chloride concentration. Bath-application of 0.1 mM picrotoxin (Ptx) decreased the depolarizing phase which was progressively replaced by a stable hyperpolarization. The transient depolarizing component desensitized quickly and was the most sensitive phase to Ptx action. The Ptx-resistant response reversed at a mean value of -100 mV close to the equilibrium potential for potassium ions (EK+), suggesting that it was generated by a K(+)-channel coupled receptor. Although baclofen was unable to mimic the Ptx-resistant GABA response, the compound CGA 147823, known to bind with a high specificity to vertebrate GABAB receptors, has been successfully used to reproduce the Ptx-resistant GABA response. It is suggested that, in addition to GABA receptors linked to chloride channels, the insect CNS possesses GABA receptors sharing ionic characteristics of GABAB receptors especially those located in the vertebrate CNS, although they are insensitive to baclofen.

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Cockroach Giant Interneurones Stained by Cobalt-Backfilling of Dissected Axons

Cited by 49 publications

References 9 publications

α-Bungarotoxin Blocks Excitatory Synaptic Transmission Between Cercal Sensory Neurones and Giant Interneurone 2 of the Cockroach,Periplaneta Americana

α-Bungarotoxin Blocks Excitatory Synaptic Transmission Between Cercal Sensory Neurones and Giant Interneurone 2 of the Cockroach,Periplaneta Americana

Acetylcholine Receptors of Identified Insect Neurons

Functional assay for GABA receptor subtypes of a cockroach giant interneuron

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