Cocirculation of two dengue virus serotypes in individual and pooled samples of Aedes aegypti and Aedes albopictus larvae

Pessanha, José Eduardo Marques; Caiaffa, Waleska Teixeira; Cecílio, Alzira Batista; Iani, Felipe Campos de Melo; Araújo, Simone Costa; Nascimento, José Carlos Rodrigues; Kroon, Erna Geessien; Proietti, Fernando Augusto; Arias, Jorge R.

doi:10.1590/s0037-86822011000100023

Cited by 35 publications

(48 citation statements)

References 12 publications

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“…Co-infection with the serotypes DENV-1/-4 and DENV-2/-4 were observed in Mato Grosso state 41 ; co-infection with DENV-1/-4, in São José do Rio Preto, São Paulo state 42 ; co-infection with DENV-1/-4, DENV-2/-4, DENV-1/-2, and DENV-3/-4, in Manaus, Amazonas state11, 43, 44; co-infection with DENV-2/-3, in Tauá, Ceará state 45 ; co-infection with DENV-1/-2, in Barretos, São Paulo state 46 ; and co-infection with DENV-1/-2, in Cuiabá, Mato Grosso state 47, 48. Co-infections with more than one serotype were also detected in Ae.…”

Section: Denv-4mentioning

confidence: 99%

Mosquito-transmitted viruses – the great Brazilian challenge

Mota

Terzian

Silva

et al. 2016

Brazilian Journal of Microbiology

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Arboviruses pose a serious threat to public health worldwide, overloading the healthcare system and causing economic losses. These viruses form a very diverse group, and in Brazil, arboviruses belonging to the families Flaviviridae and Togaviridae are predominant. Unfortunately, the number of arboviruses increases in proportion with factors such as deforestation, poor sanitation, climate changes, and introduction of new viruses like Chikungunya virus and Zika virus.In Brazil, dengue is endemic, along with the presence of other arboviruses. The situation is complicated by the scarcity of diagnostic infrastructure and the absence of approved vaccines for these diseases. Disease control, thus, relies solely on vector control. Therefore, enhanced clinical knowledge and improved general awareness about these arboviruses are indispensable to tackle diagnostic inadequacies.

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Section: Denv-4mentioning

confidence: 99%

Mosquito-transmitted viruses – the great Brazilian challenge

Mota

Terzian

Silva

et al. 2016

Brazilian Journal of Microbiology

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“…albopictus could be infected with more than 20 types of arboviruses, drawing attention to its broad vector competence, although its role in natural transmission is uncertain (Gubler et al, 2001, Vega-Rua et al, 2013). Most relevant for our work, Pessanha et al (2011) and Martins et al (2012) demonstrated the occurrence of DENV-infected Ae. albopictus larva in Brazilian cities including in the city of our study.…”

Section: Introductionmentioning

confidence: 83%

“…albopictus females, which achieved the value of 84.0%. Pessanha et al (2011) valuating three districts of Belo Horizonte (Central-South, East and Venda Nova) verified that 50.0% of larvae of Ae. albopictus were infected by Dengue virus .…”

Section: Discussionmentioning

confidence: 99%

“…Even so, the discovery of DENV-infected larvae of Ae. albopictus in the Brazilian states of Minas Gerais, Pernambuco and Roraima, located respectively in the southeast, northeast and west of the country, has drawn attention (Pessanha et al, 2011, Antunes et al, 2014, Deininger et al, 2014, Villela and Natal, 2014). In 2012, Martins et al (2012) found for the first time Ae.…”

Section: Introductionmentioning

confidence: 99%

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Aedes ( Stegomyia ) albopictus ’ dynamics influenced by spatiotemporal characteristics in a Brazilian dengue-endemic risk city

et al. 2016

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Brazil reported the majority of the dengue cases in Americas during the last two decades, where the occurrence of human dengue cases is exclusively attributed to the Aedes (Stegomyia) aegypti (Linnaeus). Nowadays, other recognized Dengue virus (DENV) vector in Asian countries, Aedes (Stegomyia) albopictus (Skuse), has been detected in more than half of the 5565 Brazilian municipalities. Therefore, the aim of the present study was to investigate the presence of, and determine the Ae. albopictus’ dynamics influenced by spatiotemporal characteristics in a dengue-endemic risk city of Belo Horizonte, Minas Gerais State’s capital. Aedes albopictus were collected across four consecutive DENV transmission seasons from 2010 to 2014. These mosquitoes were caught in three selected districts, which had been reported in the previous ten years as having high mosquito densities and an elevated concentration of human dengue cases during epidemic seasons. All field-caught Ae. albopictus was individually processed by real-time RT-PCR, to research the DENV presence. The third season (p < 0.05) and the Pampulha district (p < 0.05) had the highest proportions of field-caught Ae. albopictus, respectively. The second season had the highest proportion of DENV-infected field-caught females (p < 0.05), but there was no difference among the proportions of DENV-infected Ae. albopictus when comparing the collection in the three districts (p = 0.98). Minimum (p = 0.004) and maximum (p < 0.0001) temperature were correlated with the field-caught Ae. albopictus in four different periods and districts. In the generalized linear model of Poisson, the field-caught DENV-infected Ae. albopictus (p = 0.005), East district (p = 0.003), minimum temperature (p < 0.0001) and relative humidity (p = 0.001) remained associated with the total number of human dengue cases. Our study demonstrated that the number of field-caught DENV-infected Ae. albopictus was inversed correlated with the number of human dengue cases. Our study raises the possibility that the DENV circulating in mosquitoes Ae. albopictus is happening in non-epidemic periods, showing that this species may be keeping only the presence of the virus in nature. Further long-term studies are necessary to better understand the role of Ae. albopictus in DENV transmission and or its vectorial competence in Belo Horizonte and in other endemic cities in Brazil and in the New World countries.

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“…Female A. aegypti may be able to transmit both viruses simultaneously 17 . Pessanha et al 21 detected A. aegypti larvae that were positive for more than 1 serotype, also showing that dual infection can be passed on to offspring via transovarial transmission.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity of dengue virus serotypes 1 and 2 in the State of Paraná, Brazil, based on a fragment of the capsid/premembrane junction region

Bona

Twerdochlib

Navarro-Silva

2012

Rev. Soc. Bras. Med. Trop.

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INTRODUCTION: The precise identification of the genetic variants of the dengue virus is important to understand its dispersion and virulence patterns and to identify the strains responsible for epidemic outbreaks. This study investigated the genetic variants of the capsid-premembrane junction region fragment in the dengue virus serotypes 1 and 2 (DENV1-2). METHODS: Samples from 11 municipalities in the State of Paraná, Brazil, were provided by the Central Laboratory of Paraná. They were isolated from the cell culture line C6/36 (Aedes albopictus) and were positive for indirect immunofluorescence. Ribonucleic acid (RNA) extracted from these samples was submitted to the reverse transcription polymerase chain reaction (RT-PCR) and nested PCR. RESULTS: RT-PCR revealed that 4 of the samples were co-infected with both serotypes. The isolated DENV-1 sequences were 95-100% similar to the sequences of other serotype 1 strains deposited in GenBank. Similarly, the isolated DENV-2 sequences were 98-100% similar to other serotype 2 sequences in GenBank. According to our neighbor-joining tree, all strains obtained in this study belonged to genotype V of DENV-1. The DENV-2 strains, by contrast, belonged to the American/Asian genotypes. CONCLUSIONS: The monitoring of circulating strains is an important tool to detect the migration of virus subtypes involved in dengue epidemics.

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Cocirculation of two dengue virus serotypes in individual and pooled samples of Aedes aegypti and Aedes albopictus larvae

Cited by 35 publications

References 12 publications

Mosquito-transmitted viruses – the great Brazilian challenge

Mosquito-transmitted viruses – the great Brazilian challenge

Aedes ( Stegomyia ) albopictus ’ dynamics influenced by spatiotemporal characteristics in a Brazilian dengue-endemic risk city

Genetic diversity of dengue virus serotypes 1 and 2 in the State of Paraná, Brazil, based on a fragment of the capsid/premembrane junction region

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