1990
DOI: 10.1002/cne.903000202
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Cochlear innervation in the developing rat: An immunocytochemical study of neurofilament and spectrin proteins

Abstract: We have studied the innervation of the developing cochlea by immunocytochemical staining of the cytoskeletal proteins, neurofilament (NF), and spectrin (brain spectrin and erythrocyte spectrin). NF immunoreactivity was seen in spiral ganglion cell bodies and their processes and in fibers of the intraganglionic spiral bundle (IGSB) on gestational day 16. NF immunoreactivity with monoclonal antibodies to NF160 and NF68 was present beneath both inner hair cells (the IHC) and outer hair cells (OHCs) on gestational… Show more

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Cited by 45 publications
(33 citation statements)
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“…The efferent innervation of the OHCs in the embryonic rat forms prenatally, which is earlier than previously believed [10]. Galinovic-Schwartz et al [24] have purified a protein (Mr = 66) of neuronal IFs defined as NF-66 which seems to correspond to a-internexin.…”
Section: Discussionmentioning
confidence: 97%
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“…The efferent innervation of the OHCs in the embryonic rat forms prenatally, which is earlier than previously believed [10]. Galinovic-Schwartz et al [24] have purified a protein (Mr = 66) of neuronal IFs defined as NF-66 which seems to correspond to a-internexin.…”
Section: Discussionmentioning
confidence: 97%
“…Moreover, such differences in immunomorphological ex pression (as estimated semiquantitatively) coincided at a time when two populations of differently immunostained spiral and vestibular ganglion cells were already present. Such a difference might show the dual origin of otic gan glion cells, the separation into type I and type II spiral ganglion cells or differences in innervation patterns from Scarpa's ganglion to the two types of vestibular hair cells [9]Immunostaining to each subtypes of NF has been per formed in the developing optic nerve [21], in the develop ing cochlea [10] and in mature spiral ganglion neurons [22]. In developmental neuronal tissue, NF-H is the last NF subtype to be expressed.…”
Section: Discussionmentioning
confidence: 99%
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“…Enhanced neurofilament content was initially noted with light and electron microscopy (Nishimura et al, 1965;Thomsen, 1966;Kellerhals et al, 1967) and neurofilament stains (Kiang et al, 1982). Subsequent analysis, with immunohistochemical techniques, showed a predominance of neurofilament 68 and 160, phosphorylated neurofilament 200, and peripherin in these same neurons (Dau and Wenthold, 1985;Ryugo, 1986, 1991;Romand et al, 1990;Hafidi et al, 1990Hafidi et al, , 1993Anniko et al, 1995). These markers are useful in vitro because other ways of classifying the cells were not possible.…”
Section: Discussionmentioning
confidence: 99%
“…After labeling auditory nerve fibers with an antibody against neurofilament-associated protein 200, which is expressed in both afferent and efferent fibers (Hafidi et al, 1990), we used laserscanning confocal microscopy to reconstruct the peripheral trajectories of the axons innervating the hair cells in five cochleas. Because there is no efferent innervation in the high-frequency region of lizard basilar papillas (Manley, 1990), the auditory nerve fibers in this region represent only afferent terminals, the distal axonal segments connecting hair cells to eighth-nerve neurons.…”
Section: Innervation Pattern Of the Gecko's Hair Cellsmentioning
confidence: 99%