Cocaine challenge increases the expression of immunoglobulin heavy chain binding protein in the rat nucleus accumbens

Ryu, In Soo; Choe, Eun Sang

doi:10.1016/j.neulet.2014.06.031

Cited by 5 publications

(4 citation statements)

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“…Since repeated cocaine exposure increases Ca 2+ influx via stimulation of glutamate receptors ( Ryu and Choe, 2014 ; Yang and Choe, 2014 ), we determined the contribution of Ca 2+ influx to the phosphorylation of DARPP32-Thr75. Intracaudate infusion of the Ca 2+ chelator, BAPTA-AM (50 nmol), significantly decreased the elevation of pDARPP32-Thr75 immunoreactivity after repeated cocaine administration ( Figure 2B ).…”

Section: Resultsmentioning

confidence: 99%

“…This increase stimulates Na + channels such as AMPA and kainate glutamate receptors in GABAergic striatal neurons and produces excitatory postsynaptic potentials, resulting in the elevated release of Ca 2+ from the internal stores ( Di Ciano and Everitt, 2001 ; Vanderschuren et al, 2005 ; Robison et al, 2013 ). Taken together, increases in membrane potentials by increasing Ca 2+ and Na + influxes in GABAergic neurons after repeated cocaine further increase cytosolic Ca 2+ concentrations via voltage-operated Ca 2+ channels in the ER ( Ryu and Choe, 2014 ), leading to DARPP32-Thr75 phosphorylation. Thus, blockade of ryanodine-sensitive Ca 2+ channels in the ER decreased the elevation of the phosphorylation in this study.…”

Section: Discussionmentioning

confidence: 99%

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Activation of Protein Kinases and Phosphatases Coupled to Glutamate Receptors Regulates the Phosphorylation State of DARPP32 at Threonine 75 After Repeated Exposure to Cocaine in the Rat Dorsal Striatum in a Ca²⁺-Dependent Manner

Kim

Ryu

Seo

et al. 2015

IJNPPY

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Background:Phosphorylation state of dopamine- and cAMP-regulated phosphoprotein, molecular weight 32kDa (DARPP32) is crucial to understand drug-mediated synaptic plasticity. In this study, mechanisms underlying repeated cocaine-stimulated phosphorylation of DARPP32 at threonine 75 (pDARPP32-Thr75) were determined by investigating the hypothesis that activation of protein kinases and phosphatases coupled to glutamate signaling is necessary for the regulation of pDARPP32-Thr75 after repeated cocaine administration.Methods:Intracaudate drug infusions into the rat dorsal striatum followed by Western immunoblot analysis were mainly performed to test this hypothesis.Results:The results demonstrated that 7 repeated daily intraperitoneal injections of cocaine (20mg/kg) upregulated the expression of pDARPP32-Thr75. Increases in the cytosolic Ca2+ concentrations followed by Ca2+-dependent protein kinase activation through stimulation of Ca2+ channels in striatal neurons were necessary for the phosphorylation. Activation of protein phosphatases further regulated the phosphorylation state by deactivating pDARPP32-Thr75 and upstream protein kinases.Conclusion:These findings suggest that activation of protein kinases and phosphatases coupled to glutamate receptors controls the phosphorylation state of DARPP32-Thr75 after repeated exposure to cocaine in the dorsal striatum in a Ca2+-dependent manner.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Activation of Protein Kinases and Phosphatases Coupled to Glutamate Receptors Regulates the Phosphorylation State of DARPP32 at Threonine 75 After Repeated Exposure to Cocaine in the Rat Dorsal Striatum in a Ca²⁺-Dependent Manner

Kim

Ryu

Seo

et al. 2015

IJNPPY

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“…The possibility of gliosis resulting from the implantation and the infusion of drugs dissolved in DMSO/aCSF was examined by performing Nissl staining (Figure 3B). 33 …”

Section: Methodsmentioning

confidence: 99%

Linking of NMDA receptors and mGluR5 in the nucleus accumbens core to repeated cocaine‐induced 50‐kHz ultrasonic vocalization in rats

et al. 2021

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Rats express a positive emotional state by emitting 50‐kHz ultrasonic vocalization (USV) calls in response to drug exposure. This study demonstrated the linking of glutamate receptors in the nucleus accumbens (NAc) to vocal expression of 50‐kHz USV calls after repeated cocaine administration in freely moving rats. Repeated systemic injections of cocaine (20 mg/kg/day, i.p.) for seven consecutive days increased the number of 50‐kHz USV calls. Intra‐NAc core infusion of the broad‐glutamate receptor antagonist, γDGG (50 nmol/side), decreased the repeated cocaine‐induced increase in the number of 50‐kHz USV calls. Intra‐NAc core infusion of the N‐methyl‐D‐aspartate (NMDA) receptor antagonist, MK801 (2 nmol/side), but not α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid or kainic acid receptor antagonist, CNQX disodium salt (2 nmol/side), decreased the number of 50‐kHz USV calls that had been elevated by repeated exposure to cocaine. Intra‐NAc core infusion of the group I metabotropic glutamate receptor subtype 5 (mGluR5), MPEP (0.5 nmol/side), MTEP (15 nmol/side) and inositol‐1,4,5‐trisphosphate receptor blocker, xestospongin C (0.004 nmol/side) decreased the cocaine‐induced increase in the number of USV calls. These data suggest that the NMDA receptor‐ and mGluR5‐dependent increase in intracellular Ca2+ concentrations in the NAc core is linked to a positive emotional state after repeated exposure to cocaine in rats.

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“…Western immunoblotting was performed as previously described [ 23 ]. For western immunoblotting, PC12 cells were plated in 6-well plates at a density of 1 × 10 4 cells/well in the culture medium for 24 h. The treated doses of L. platyphylla extract and nicotine following in vitro study were determined from the results of a cell viability test.…”

Section: Methodsmentioning

confidence: 99%

Mitigating Effects of Liriope platyphylla on Nicotine-Induced Behavioral Sensitization and Quality Control of Compounds

et al. 2020

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In this study we investigated the mitigating effects of Liriope platyphylla Wang et Tang extract on behavioral sensitization and the quantification of its major compounds. The extract of L. platyphylla reduces the expression of tyrosine hydroxylase (TH) protein, which is increased by nicotine, back to normal levels, and increases the expression of dopamine transporter (DAT) protein, which is reduced by nicotine, back to normal levels in PC12 cells. In this study, rats received nicotine (0.4 mg/kg, subcutaneously) only for seven days and then received extract of L. platyphylla (200 or 400 mg/kg, oral) 1 h prior to nicotine administration for an additional seven days. The extract of L. platyphylla reduced locomotor activity compared to the nicotine control group in rats. The extract of L. platyphylla significantly attenuated the repeated nicotine-induced DAT protein expression in the nucleus accumbens (NAc), but there was no effect on increased TH protein expression in the dorsal striatum. These findings suggest that L. platyphylla extract has a mitigating effect on nicotine-induced behavioral sensitization by modulating DAT protein expression in the NAc. For quality control of L. plathyphylla, spicatoside A and D, which are saponin compounds, were quantified in the L. platyphylla extract. The amounts of spicatoside A and D in L. platyphylla extract obtained from ultra-high-performance liquid chromatography with tandem mass spectrometry were 0.148 and 0.272 mg/g, respectively. The identification of these compounds in L. platyphylla, which can be used for quality control, provides important information for the development of drugs to treat nicotine dependence.

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Cocaine challenge increases the expression of immunoglobulin heavy chain binding protein in the rat nucleus accumbens

Cited by 5 publications

References 24 publications

Activation of Protein Kinases and Phosphatases Coupled to Glutamate Receptors Regulates the Phosphorylation State of DARPP32 at Threonine 75 After Repeated Exposure to Cocaine in the Rat Dorsal Striatum in a Ca²⁺-Dependent Manner

Activation of Protein Kinases and Phosphatases Coupled to Glutamate Receptors Regulates the Phosphorylation State of DARPP32 at Threonine 75 After Repeated Exposure to Cocaine in the Rat Dorsal Striatum in a Ca²⁺-Dependent Manner

Linking of NMDA receptors and mGluR5 in the nucleus accumbens core to repeated cocaine‐induced 50‐kHz ultrasonic vocalization in rats

Mitigating Effects of Liriope platyphylla on Nicotine-Induced Behavioral Sensitization and Quality Control of Compounds

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Cocaine challenge increases the expression of immunoglobulin heavy chain binding protein in the rat nucleus accumbens

Cited by 5 publications

References 24 publications

Activation of Protein Kinases and Phosphatases Coupled to Glutamate Receptors Regulates the Phosphorylation State of DARPP32 at Threonine 75 After Repeated Exposure to Cocaine in the Rat Dorsal Striatum in a Ca2+-Dependent Manner

Activation of Protein Kinases and Phosphatases Coupled to Glutamate Receptors Regulates the Phosphorylation State of DARPP32 at Threonine 75 After Repeated Exposure to Cocaine in the Rat Dorsal Striatum in a Ca2+-Dependent Manner

Linking of NMDA receptors and mGluR5 in the nucleus accumbens core to repeated cocaine‐induced 50‐kHz ultrasonic vocalization in rats

Mitigating Effects of Liriope platyphylla on Nicotine-Induced Behavioral Sensitization and Quality Control of Compounds

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Product

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Activation of Protein Kinases and Phosphatases Coupled to Glutamate Receptors Regulates the Phosphorylation State of DARPP32 at Threonine 75 After Repeated Exposure to Cocaine in the Rat Dorsal Striatum in a Ca²⁺-Dependent Manner

Activation of Protein Kinases and Phosphatases Coupled to Glutamate Receptors Regulates the Phosphorylation State of DARPP32 at Threonine 75 After Repeated Exposure to Cocaine in the Rat Dorsal Striatum in a Ca²⁺-Dependent Manner