Cocaine alters Homer1 natural antisense transcript in the nucleus accumbens

Sartor, Gregory C.; Powell, Samuel K.; Velmeshev, Dmitry; Lin, David T.W.; Magistri, Marco; Giudice, Jimena; Malvezzi, Andrea M.; Andrade, Nadja S.; Faghihi, Mohammad Ali; Wahlestedt, Claes

doi:10.1016/j.mcn.2017.10.003

Cited by 6 publications

(4 citation statements)

References 52 publications

(75 reference statements)

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“…We firstly identified DElncRNAs, DEmiRNAs and DEmRNAs in morphine addiction. Although these lncRNAs have not been reported to be involved in drug addiction, dysregulated expression of lncRNAs has been reported to play a critical role in the progression of addiction ( 26 , 27 ). Gao et al showed that upregulation of MEG3 was involved in morphine-induced autophagy in hippocampal neuronal HT22 cells ( 28 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis of Long Non-coding RNA-MicroRNA-mRNA Interactions in the Nucleus Accumbens Related to Morphine Addiction in Mice

Xie

et al. 2022

Front. Psychiatry

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Recent research suggest that some non-coding RNAs (ncRNAs) are important regulators of chromatin dynamics and gene expression in nervous system development and neurological diseases. Nevertheless, the molecular mechanisms of long non-coding RNAs (lncRNAs), acting as competing endogenous RNAs (ceRNAs), underlying morphine addiction are still unknown. In this research, RNA sequencing (RNA-seq) was used to examine the expression profiles of lncRNAs, miRNAs and mRNAs on the nucleus accumbens (NAc) tissues of mice trained with morphine or saline conditioned place preference (CPP), with differential expression of 31 lncRNAs, 393 miRNAs, and 371 mRNAs found. A ceRNA network was established for reciprocal interactions for 9 differentially expressed lncRNAs (DElncRNAs), 10 differentially expressed miRNAs (DEmiRNAs) and 12 differentially expressed mRNAs (DEmRNAs) based on predicted miRNAs shared by lncRNAs and mRNAs. KEGG pathway enrichment analyses were conducted to explore the potential functions of DEmRNAs interacting with lncRNAs in the ceRNA network. These DEmRNAs were enriched in synaptic plasticity-related pathways, including pyrimidine metabolism, ECM-receptor interaction, and focal adhesion. The correlation between the relative expression of lncRNAs, miRNAs and mRNAs was analyzed to further validate predicted ceRNA networks, and the Lnc15qD3-miR-139-3p-Lrp2 ceRNA regulatory interaction was determined. These results suggest that the comprehensive network represents a new insight into the lncRNA-mediated ceRNA regulatory mechanisms underlying morphine addiction and provide new potential diagnostic and prognostic biomarkers for morphine addiction.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis of Long Non-coding RNA-MicroRNA-mRNA Interactions in the Nucleus Accumbens Related to Morphine Addiction in Mice

Xie

et al. 2022

Front. Psychiatry

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“…However, a study revealed an increase in the expression of HOTAIRM1 in differentiating neurons, suggesting a role in early neurogenesis and brain development that may be disrupted by prenatal cocaine exposure (Lin et al, 2011). Another transcriptomic study also found an increase in Homer1 antisense (Homer1-AS) in the nucleus accumbens of mice after repeated cocaine exposure that in vitro was demonstrated to increase Homer1 sense expression (Sartor et al, 2017). Homer1 is an immediate-early gene and a scaffold protein found in postsynaptic densities that is associated with plasticity and spine morphogenesis (Shiraishi-Yamaguchi & Furuichi, 2007), meaning abnormal expression of Homer1-AS and subsequent dysregulation of Homer1 as a result of prenatal cocaine exposure may contribute to some of the abnormal neural development associated with prenatal cocaine exposure.…”

Section: Cocainementioning

confidence: 99%

Noncoding RNAs in development and teratology, with focus on effects of cannabis, cocaine, nicotine, and ethanol

Pinson

Miranda

2019

Birth Defects Research

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Completion of the Human Genome Project has led to the identification of a large number of transcription start sites that are not paired with protein‐coding genes, supporting the growing recognition of the abundance of encoded nonprotein‐coding RNAs (ncRNAs) and their importance for speciation and species‐specific development. Present in both plants and animals, ncRNAs vary in size, function, primary sequence, and secondary structure. While microRNAs (miRNAs) are the best known, there are a number of other ncRNAs (long[er] nonprotein‐coding RNA, pseudogenes, circular RNAs, and so on) that have been shown to play an important role in the development either directly or via networks of proteins and other ncRNAs, including modulating the impact of miRNAs. Furthermore, these ncRNAs and their developmental regulatory networks are sensitive to teratogens such as ethanol, cannabis, cocaine, and nicotine. A better understanding of the developmental role of ncRNAs and their capacity to mediate teratogenesis is a necessary step in efforts to minimize the long‐term consequences of developmental exposures to drugs‐of‐abuse. Moreover, with increasing awareness of the prevalence of polydrug use, experimental models will need to incorporate more complex drug exposure paradigms into meaningful assessments of developmental ncRNA function.

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“…Additionally, they found evidence that these corresponding changes in NAc lncRNA might correspond with changes in addiction-associated mRNA ( Zhu et al, 2016 ). Further research has characterized a specific lncRNA that is antisense to a synaptically localized addiction-related gene, Homer1 ( Sartor et al, 2017 ). After repeated injections of cocaine, it was found that Homer1-AS was increased within the NAc.…”

Section: Ncrna Regulation Of Substance Use Disorders (Suds)mentioning

confidence: 99%

Regulation of Adult Neurogenesis by Non-coding RNAs: Implications for Substance Use Disorders

Oliver

Mandyam

2018

Front. Neurosci.

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The discovery of non-coding RNAs (ncRNAs)has been one of the central findings from early genomic sequencing studies. Not only was the presence of these genes unknown previously, it was the staggering disproportionate share of the genome that was predicted to be encoded by ncRNAs that was truly significant in genomic research. Over the years the function of various classes of these ncRNAs has been revealed. One of the first and enduring regulatory programs associated with these factors was development. In the neurosciences, the discovery of adult derived populations of dividing cells within the brain was equally substantial. The brain was hypothesized to be plastic only in its neuronal connectivity, but the discovery of the generation of new neurons was a novel mechanism of neuronal and behavioral plasticity. The process of adult neurogenesis resembles early neuronal development and has been found to share many parallels in the proper stages of specified genetic programs. Adult neurogenesis has also been found to play a role in learning and memory involved in particular hippocampal-dependent behaviors. Substance use disorders (SUDs) are an example of a behavioral condition that is associated with and possibly driven by hippocampal alterations. Our laboratory has determined that hippocampal adult neurogenesis is necessary for a rodent model of methamphetamine relapse. Due to the previous research on ncRNAs in development and in other brain regions involved in SUDs, we posit that ncRNAs may play a role in adult neurogenesis associated with this disorder. This review will cover the regulatory mechanisms of various classes of ncRNAs on the coordinated genetic program associated with adult neurogenesis with a special focus on how these programs could be dysregulated in SUDs.

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Cocaine alters Homer1 natural antisense transcript in the nucleus accumbens

Cited by 6 publications

References 52 publications

Transcriptomic Analysis of Long Non-coding RNA-MicroRNA-mRNA Interactions in the Nucleus Accumbens Related to Morphine Addiction in Mice

Transcriptomic Analysis of Long Non-coding RNA-MicroRNA-mRNA Interactions in the Nucleus Accumbens Related to Morphine Addiction in Mice

Noncoding RNAs in development and teratology, with focus on effects of cannabis, cocaine, nicotine, and ethanol

Regulation of Adult Neurogenesis by Non-coding RNAs: Implications for Substance Use Disorders

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