COBLL1 modulates cell morphology and facilitates androgen receptor genomic binding in advanced prostate cancer

Takayama, Ken Ichi; Suzuki, Takashi; Fujimura, Tetsuya; Takahashi, Satoru; Inoue, Satoshi

doi:10.1073/pnas.1721957115

Cited by 23 publications

(33 citation statements)

References 39 publications

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“…Prostate cancer is one of the most common cancers diagnosed in developed countries . Despite an increased ability to detect PC and the presence of various types of treatment, including androgen deprivation therapy, PC could still be lethal after it progresses to CRPC . Multiple mechanisms are proposed for AR involvement and the development of CRPC .…”

Section: Introductionmentioning

confidence: 99%

“…Despite an increased ability to detect PC and the presence of various types of treatment, including androgen deprivation therapy, PC could still be lethal after it progresses to CRPC . Multiple mechanisms are proposed for AR involvement and the development of CRPC . Nevertheless, in men with metastatic disease, the prognosis is poor with an average overall survival of 24‐48 months .…”

Section: Introductionmentioning

confidence: 99%

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Androgen‐responsive tripartite motif 36 enhances tumor‐suppressive effect by regulating apoptosis‐related pathway in prostate cancer

et al. 2018

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Tripartite motif 36 (TRIM36) belongs to the TRIM family, most members of which are involved in ubiquitination and degradation of target proteins by functioning as E3 ubiquitin ligases. The function of TRIM36 has not been well documented, therefore, we investigated the clinical significance and function of TRIM36 in human prostate cancer (PC). Multivariate logistic regression analysis showed that TRIM36 immunoreactivity was an independent predictor of cancer‐specific survival of PC patients. Gain‐of‐function study revealed that overexpression of TRIM36 suppressed cell proliferation and migration of LNCaP, 22Rv1, and DU145 cells. Moreover, TRIM36 knockdown using siRNA suppressed apoptosis and promoted cell proliferation and migration in LNCaP and 22Rv1 cells. Furthermore, our microarray analysis revealed that the apoptosis‐related pathway was significantly upregulated by TRIM36 overexpression. The TUNEL assay showed that apoptosis promoted by docetaxel treatment was alleviated in siTRIM36‐treated LNCaP and 22Rv1 cells. Taken together, these results suggest that high expression of TRIM36 is associated with favorable prognosis and that TRIM36 plays a tumor‐suppressive role by inhibiting cell proliferation and migration as well as promoting apoptosis in PC.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Androgen‐responsive tripartite motif 36 enhances tumor‐suppressive effect by regulating apoptosis‐related pathway in prostate cancer

et al. 2018

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“…In order to demonstrate the direct regulation of APP by AR, we surveyed the AR ChIP‐seq data (Takayama, Suzuki, Fujimura, Takahashi, & Inoue, , or unpublished data) and histone modification patterns in the APP locus for the identification of several candidate AR‐binding sites. When an AR ChIP analysis was carried out, we found that one ARBS in the APP intronic region was functional in the SH‐SY5Y cells (Figure a).…”

Section: Resultsmentioning

confidence: 99%

“…ChIP and quantitative PCR (qPCR) were conducted as previously described (Takayama et al, , ). The fold enrichment relative to input was measured by performing qPCR using the KAPA SYBR Green PCR Master Mix (Sigma Genosys) and Applied Biosystems StepOne.…”

Section: Methodsmentioning

confidence: 99%

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Amyloid precursor protein, an androgen‐regulated gene, is targeted by RNA‐binding protein PSF/SFPQ in neuronal cells

2019

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Amyloid precursor protein (APP) is a representative gene related to Alzheimer's disease (AD). Androgens function by binding to the androgen receptor (AR). Both androgen and RNA‐binding protein PSF play a role in the pathology of AD. However, the involvement of AR and PSF in APP regulation in neuron has not been investigated. Here, we explored the regulatory mechanism of APP expression by AR and PSF using neuron‐derived cells. We demonstrated that androgen up‐regulates the production of APP at the mRNA and protein levels. This induction is enhanced by AR over‐expression and inhibited by its silencing. One candidate AR‐binding region was identified in the intron region of APP and validated its activity as AR‐dependent enhancer by the luciferase assay. Furthermore, the public transcriptome data of brain tissues of mice indicated that APP is regulated by PSF post‐transcriptionally. We observed a decreased expression of APP after PSF knockdown and interaction of PSF with the APP transcript. Moreover, we revealed that silencing of PSF inhibited the stability of the APP mRNA. Thus, these results presented a new regulatory mechanism of APP expression by androgen through AR‐mediated transcription and PSF at the post‐transcriptional level that might be associated with the occurrence of AD.

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Reciprocal interactions among Cobll1, PACSIN2, and SH3BP1 regulate drug resistance in chronic myeloid leukemia

Park

Yoo

et al. 2022

Cancer Medicine

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Cobll1 affects blast crisis (BC) progression and tyrosine kinase inhibitor (TKI) resistance in chronic myeloid leukemia (CML). PACSIN2, a novel Cobll1 binding protein, activates TKI-induced apoptosis in K562 cells, and this activation is suppressed by Cobll1 through the interaction between PACSIN2 and Cobll1.PACSIN2 also binds and inhibits SH3BP1 which activates the downstream Rac1 pathway and induces TKI resistance. PACSIN2 competitively interacts with Cobll1 or SH3BP1 with a higher affinity for Cobll1. Cobll1 preferentially binds to PACSIN2, releasing SH3BP1 to promote the SH3BP1/Rac1 pathway and suppress

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COBLL1 modulates cell morphology and facilitates androgen receptor genomic binding in advanced prostate cancer

Cited by 23 publications

References 39 publications

Androgen‐responsive tripartite motif 36 enhances tumor‐suppressive effect by regulating apoptosis‐related pathway in prostate cancer

Androgen‐responsive tripartite motif 36 enhances tumor‐suppressive effect by regulating apoptosis‐related pathway in prostate cancer

Amyloid precursor protein, an androgen‐regulated gene, is targeted by RNA‐binding protein PSF/SFPQ in neuronal cells

Reciprocal interactions among Cobll1, PACSIN2, and SH3BP1 regulate drug resistance in chronic myeloid leukemia

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