COBL9 and COBL7 synergistically regulate root hair tip growth via controlling apical cellulose deposition

Li, Zhengzheng; Zhou, Tao; Sun, Peng; Chen, Xinhang; Gong, Luping; Ge, Shengchao; Liang, You-Sheng

doi:10.1016/j.bbrc.2022.01.096

Cited by 9 publications

(9 citation statements)

References 31 publications

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“…The kojak mutant produces a normal bulge during the beginning of the root hair cell formation process, but this bulge prematurely swells and ruptures, eventually failing to form normal root hair, which suggests that the gene plays a role after the initiation stage . Two proteins from the COBL family, COBL9 and COBL7, are involved in cellulose biosynthesis and processing; changes in their expression levels, together with those of AtPRPL1 , a proline-rich protein, can also interfere with normal root hair growth . REPRESSOR OF EXCESSIVE ROOT HAIR GROWTH 1 (RXR1), a protein possessing an unknown functional domain known as DUF506, also negatively regulates root hair elongation …”

Section: Molecular Mechanisms Underlying Root Hair Developmentmentioning

confidence: 99%

Root Hair Development and Adaptation to Abiotic Stress

Zhang,

Yang,

Zhang

et al. 2023

J. Agric. Food Chem.

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Root hairs tie the root system to the soil substrate, facilitate water and nutrient absorption, and enable the interaction with microbes in the soil. Root hair development can be classified into three main development types (I−III). Root hair development type III has been extensively studied, mainly represented using the model plant Arabidopsis thaliana. Transcription factors, plant hormones, and proteins are involved at different root hair developmental stages. The mechanisms underlying development in types I and II have been examined using other representative plant species but have not been studied as intensively.Many key developmental genes in types I and II are highly homologous with those in type III, exhibiting conservation of related mechanisms. Root hairs are also involved in the regulation of plant response to abiotic stress by altering developmental patterns. Abiotic stress, regulatory genes, and plant hormones jointly regulate root hair development and growth; however, few studies have focused on how root hair recognizes abiotic stress signals. This review examines the molecular mechanisms of root hair development and adaptations under stress, and prospective future developments in root hair research are also discussed.

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Section: Molecular Mechanisms Underlying Root Hair Developmentmentioning

confidence: 99%

Root Hair Development and Adaptation to Abiotic Stress

Zhang,

Yang,

Zhang

et al. 2023

J. Agric. Food Chem.

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“…XTH positively regulated plant salt stress tolerance by regulating plant architecture ( Ishida and Yokoyama, 2022 ). COBL9 and COBL7 , two COBRA-like family genes, are required for salinity tolerance and COBL9 positively regulated root hair elongation and salinity tolerance in Arabidopsis ; the rice counterparts of these genes, OsBC1L1 and OsBC1L8 , function redundantly in response to salinity stress ( Li et al, 2022 ). Pectin plays an important role in adaptation to salt stress by regulating cell adhesion and tissue cohesion.…”

Section: Discussionmentioning

confidence: 99%

Combined transcriptomic and metabolomic analysis reveals a role for adenosine triphosphate-binding cassette transporters and cell wall remodeling in response to salt stress in strawberry

Chang

Sun

et al. 2022

Front. Plant Sci.

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Strawberry (Fragaria × ananassa Duch) are sensitive to salt stress, and breeding salt-tolerant strawberry cultivars is the primary method to develop resistance to increased soil salinization. However, the underlying molecular mechanisms mediating the response of strawberry to salinity stress remain largely unknown. This study evaluated the salinity tolerance of 24 strawberry varieties, and transcriptomic and metabolomic analysis were performed of ‘Sweet Charlie’ (salt-tolerant) and ‘Benihoppe’ (salt-sensitive) to explore salt tolerance mechanisms in strawberry. Compared with the control, we identified 3412 differentially expressed genes (DEGs) and 209 differentially accumulated metabolites (DAMs) in ‘Benihoppe,’ and 5102 DEGs and 230 DAMs in ‘Sweet Charlie.’ DEGs Gene Ontology (GO) enrichment analyses indicated that the DEGs in ‘Benihoppe’ were enriched for ion homeostasis related terms, while in ‘Sweet Charlie,’ terms related to cell wall remodeling were over-represented. DEGs related to ion homeostasis and cell wall remodeling exhibited differential expression patterns in ‘Benihoppe’ and ‘Sweet Charlie.’ In ‘Benihoppe,’ 21 ion homeostasis-related DEGs and 32 cell wall remodeling-related DEGs were upregulated, while 23 ion homeostasis-related DEGs and 138 cell wall remodeling-related DEGs were downregulated. In ‘Sweet Charlie,’ 72 ion homeostasis-related DEGs and 275 cell wall remodeling-related DEGs were upregulated, while 11 ion homeostasis-related DEGs and 20 cell wall remodeling-related DEGs were downregulated. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses showed only four KEGG enriched pathways were shared between ‘Benihoppe’ and ‘Sweet Charlie,’ including flavonoid biosynthesis, phenylalanine metabolism, phenylpropanoid biosynthesis and ubiquinone, and other terpenoid-quinone biosynthesis. Integrating the results of transcriptomic and metabolomics analyses showed that adenosine triphosphate-binding cassette (ABC) transporters and flavonoid pathway genes might play important roles in the salt stress response in strawberry, and DAMs and DEGs related to ABC transporter and flavonoid pathways were differentially expressed or accumulated. The results of this study reveal that cell wall remodeling and ABC transporters contribute to the response to salt stress in strawberry, and that related genes showed differential expression patterns in varieties with different salt tolerances. These findings provide new insights into the underlying molecular mechanism of strawberry response to salt stress and suggest potential targets for the breeding of salt-tolerant strawberry varieties.

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“…Citrine CDS was amplified from Pro COBL10::SP‐citrine‐COBL10 plasmid (Li et al ., 2013). The SP‐citrine‐COBL7/8 were obtained according to a three‐step PCR protocol (Tian et al ., 2004; Li et al ., 2022), before inserted into pK2GW7 through LR recombination reaction. Then, the 35S promoters were replaced by the respective native promoter to generate Pro COBL7/8::SP‐citrine‐COBL7/8 .…”

Section: Methodsmentioning

confidence: 99%

“…COBL7 belongs to the COBL gene family, which encode GPIanchored proteins and play a role in cell wall expansion and cellulose deposition (Schindelman et al, 2001;Roudier et al, 2002Roudier et al, , 2005. The functionality of COBL7 is poorly understood beyond a descriptive study in root hair growth (Li et al, 2022). The moderate deficient phenotype of cobl7 in stomatal development suggests a possibility that COBL genes are functionally redundant.…”

Section: Cobl7 Plays a Predominant Role And Functions Redundantly Wit...mentioning

confidence: 99%

COBL7 is required for stomatal formation via regulation of cellulose deposition in Arabidopsis

Ge,

Sun,

et al. 2023

New Phytologist

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Summary As a key regulator of plant photosynthesis, water use efficiency and immunity, stomata are specialized cellular structures that adopt defined shapes. However, our knowledge about the genetic players of stomatal pore formation and stomatal morphogenesis remains limited. Forward genetic screening, positional cloning, confocal and electron microscopy, physiological and pharmacological assays were employed for isolation and characterization of mutants and genes. We identified a mutant, dsm1, with impaired cytokinesis and deformed stomata. DSM1 is highly expressed in guard mother cells and guard cells, and encodes COBRA‐LIKE 7 (COBL7), a plant‐specific glycosylphosphatidylinositol (GPI)‐anchored protein. COBRA‐LIKE 7 and its closest homologue, COBL8, are first enriched on the forming cell plates during cytokinesis, and then their subcellular distribution and abundance change are correlated with the progressive stages of stomatal pore formation. Both COBL7 and COBL8 possess an ability to bind cellulose. Perturbing the expression of COBL7 and COBL8 leads to a decrease in cellulose content and inhibition of stomatal pore development. Moreover, we found that COBL7, COBL8 and CSLD5 have synergistic effects on stomatal development and plant growth. Our findings reveal that COBL7 plays a predominant and functionally redundant role with COBL8 in stomatal formation through regulating cellulose deposition and ventral wall modification in Arabidopsis.

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COBL9 and COBL7 synergistically regulate root hair tip growth via controlling apical cellulose deposition

Cited by 9 publications

References 31 publications

Root Hair Development and Adaptation to Abiotic Stress

Root Hair Development and Adaptation to Abiotic Stress

Combined transcriptomic and metabolomic analysis reveals a role for adenosine triphosphate-binding cassette transporters and cell wall remodeling in response to salt stress in strawberry

COBL7 is required for stomatal formation via regulation of cellulose deposition in Arabidopsis

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