Cobalt oxide magnetic nanoparticles–chitosan nanocomposite based electrochemical urea biosensor

Ali, Abderrahman Ait; Israr-Qadir, Muhammad; Wazir, Z.; Tufail, Muhammad; Ibupoto, Zafar Hussain; Jamil-Rana, Sadaf; Khan, Shahid Ali; Willander, Magnus

doi:10.1007/s12648-014-0594-3

Cited by 27 publications

(19 citation statements)

References 35 publications

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“…These results suggest that immobilization stabilizes the structure of the peptide and helps to maintain the catalytic activity of the immobilized enzyme at a high level . This could be explained by the fact that immobilization leads to increased rigidity of the enzyme particle and quenching of the entire structure …”

Section: Resultsmentioning

confidence: 88%

“…The presence of hydroxyl and amino groups in its molecules facilitates the creation of strong and stable covalent bonds between the enzyme and the support, and like chitin, chitosan can easily be obtained in a variety of forms . There are also many reports concerning chitosan particles combined with various metal oxides, such as ZnO, Fe 3 O 4 , and Co 3 O 4 , as well as other materials, including graphene oxide and alginate . The use of these materials as supports in enzyme immobilization enables the binding of a larger quantity of the peptides and entails easier control of the catalyzed process.…”

Section: Introductionmentioning

confidence: 99%

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Luffa cylindrica sponges as a thermally and chemically stable support for Aspergillus niger lipase

Zdarta

Jesionowski

2016

Biotechnology Progress

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The use of biopolymer compounds as matrices for enzyme immobilization is currently a focus of increasing interest. In the present work we propose the use of Luffa cylindrica vegetable sponges as a support for the lipase extracted from Aspergillus niger. Effectiveness of immobilization was analyzed using Fourier transform infrared spectroscopy, elemental analysis and the Bradford method. An initial enzyme solution concentration of 1.0 mg/mL and an immobilization time of 12 h were selected as the parameters that produce a system retaining the highest hydrolytic activity (84% of free enzyme). The resulting biocatalyst system also exhibited high thermal and chemical stability, reusability and storage stability, which makes it a candidate for use in a wide range of applications. Kinetic parameters for the native and immobilized lipase were also calculated. The value of the Michaelis-Menten constant for the immobilized lipase (0.47 mM) is higher than for the free enzyme (0.21 mM), which indicates that the adsorbed enzyme exhibits a lower affinity to the substrate than native lipase. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:657-665, 2016.

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Section: Resultsmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Luffa cylindrica sponges as a thermally and chemically stable support for Aspergillus niger lipase

Zdarta

Jesionowski

2016

Biotechnology Progress

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“…9 C, showed a maximum interference of 2.1% with the presence of ascorbic acid. The results illustrated the importance of the prepared biosensor (FTO/ZnO/Urs) in the selective detection of urea.Finally, based on the enzyme electrostatic immobilization strategy, the response characteristics of the FTO/ZnO/Urs biosensor with the other reported urea biosensors was compared and summarized inTable 2[45][46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61][62][63]. We are currently developing nanostructured ZnO based urea biosensing using impedimetric assessment.…”

mentioning

confidence: 99%

Disposable urea biosensor based on nanoporous ZnO film fabricated from omissible polymeric substrate

Rahmanian

Mozaffari

Abedi

2015

Materials Science and Engineering: C

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“…Wazir et al developed a potentiometric urea biosensor fabricated on glass filter paper through the immobilization of urease enzyme onto cobalt oxide- chitosan nanocomposite. The sensitivity was measured over the concentration range between 1 × 10 −4 and 8 × 10 −2 M of the urea electrolyte solution, revealing that the fabricated biosensor exhibited good sensing ability with a linear slope curve of 45 mV/decade [ 151 ]. Ge et al constructed a Co 3 O 4 -Au polyhedron-based photoelectrochemical (PEC) biosensor for detecting miRNA-141 detection with a linear range of 1 pM to 50 nM, and a detection limit of 0.2 pM [ 152 ].…”

Section: Nanomaterial-based Biosensors (Nanobiosensors)mentioning

confidence: 99%

A Review on Biosensors and Recent Development of Nanostructured Materials-Enabled Biosensors

Naresh

Lee

2021

Sensors

936

427

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A biosensor is an integrated receptor-transducer device, which can convert a biological response into an electrical signal. The design and development of biosensors have taken a center stage for researchers or scientists in the recent decade owing to the wide range of biosensor applications, such as health care and disease diagnosis, environmental monitoring, water and food quality monitoring, and drug delivery. The main challenges involved in the biosensor progress are (i) the efficient capturing of biorecognition signals and the transformation of these signals into electrochemical, electrical, optical, gravimetric, or acoustic signals (transduction process), (ii) enhancing transducer performance i.e., increasing sensitivity, shorter response time, reproducibility, and low detection limits even to detect individual molecules, and (iii) miniaturization of the biosensing devices using micro-and nano-fabrication technologies. Those challenges can be met through the integration of sensing technology with nanomaterials, which range from zero- to three-dimensional, possessing a high surface-to-volume ratio, good conductivities, shock-bearing abilities, and color tunability. Nanomaterials (NMs) employed in the fabrication and nanobiosensors include nanoparticles (NPs) (high stability and high carrier capacity), nanowires (NWs) and nanorods (NRs) (capable of high detection sensitivity), carbon nanotubes (CNTs) (large surface area, high electrical and thermal conductivity), and quantum dots (QDs) (color tunability). Furthermore, these nanomaterials can themselves act as transduction elements. This review summarizes the evolution of biosensors, the types of biosensors based on their receptors, transducers, and modern approaches employed in biosensors using nanomaterials such as NPs (e.g., noble metal NPs and metal oxide NPs), NWs, NRs, CNTs, QDs, and dendrimers and their recent advancement in biosensing technology with the expansion of nanotechnology.

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Cobalt oxide magnetic nanoparticles–chitosan nanocomposite based electrochemical urea biosensor

Cited by 27 publications

References 35 publications

Luffa cylindrica sponges as a thermally and chemically stable support for Aspergillus niger lipase

Luffa cylindrica sponges as a thermally and chemically stable support for Aspergillus niger lipase

Disposable urea biosensor based on nanoporous ZnO film fabricated from omissible polymeric substrate

A Review on Biosensors and Recent Development of Nanostructured Materials-Enabled Biosensors

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