Cobalt ions influence proliferation and function of human osteoblast-like cells

Anissian, Lucas; Stark, André; Dahlstrand, Henrik; Granberg, B.; Good, Victoria; Bucht, E.

doi:10.1080/000164702320155400

Cited by 62 publications

(50 citation statements)

References 19 publications

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“…28,29 Cytotoxic effects of cobalt ions have been demonstrated in macrophages, with cobalt ions inducing apoptosis, necrosis, and TNF-a secretion. [30][31][32][33][34] A similar cytotoxic effect has been observed in osteoblast-like cells, with reduced osteoblast proliferation, [35][36][37] viability, 35,37 and function 35,36,38 observed. In this study we investigated the in vitro effect of cobalt ions on primary human osteoblasts (pHOBs).…”

mentioning

confidence: 54%

Cobalt ions induce chemokine secretion in primary human osteoblasts

Queally¹,

Devitt²,

Butler³

et al. 2009

Journal Orthopaedic Research

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Chemokines are major regulators of the inflammatory response and have been shown to play an important role in periprosthetic osteolysis. Titanium particles have previously been shown to induce IL-8 and MCP-1 secretion in osteoblasts. These chemokines result in the chemotaxis and activation of neutrophils and macrophages, respectively. Despite a resurgence in the use of cobalt-chromium-molybdenum alloys in metal-on-metal arthroplasty, cobalt and chromium ion toxicity in the periprosthetic area has been insufficiently studied. In this study we investigate the in vitro effect of cobalt ions on primary human osteoblast activity. We demonstrate that cobalt ions rapidly induce the protein secretion of IL-8 and MCP-1 in primary human osteoblasts. This elevated chemokine secretion is preceded by an increase in the transcription of the corresponding chemokine gene. Using a Transwell migration chemotaxis assay we also demonstrate that the chemokines secreted are capable of inducing neutrophil and macrophage migration. Furthermore, cobalt ions significantly inhibit osteoblast function as demonstrated by reduced alkaline phosphatase activity and calcium deposition. In aggregate these data demonstrate that cobalt ions can activate transcription of the chemokine genes IL-8 and MCP-1 in primary human osteoblasts. Cobalt ions are not benign and may play an important role in the pathogenesis of osteolysis by suppressing osteoblast function and stimulating the production and secretion of chemokines that attract inflammatory and osteoclastic cells to the periprosthetic area. ß

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mentioning

confidence: 54%

Cobalt ions induce chemokine secretion in primary human osteoblasts

Queally¹,

Devitt²,

Butler³

et al. 2009

Journal Orthopaedic Research

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“…Osteoblasts exposed to Co and Cr ions undergo a dose-dependent reduction in proliferation. Although the metal ion concentrations within bone in vivo has not been established, exposure of osteoblasts to Co particles at concentrations of 100 µg/L or higher leads to the development of cytoplasmic vacuolations (Allen et al 1997, Anissian et al 2002, Hallab et al 2002. Moreover, Co and Cr ions are toxic for osteoblasts, leading to markedly reduced alkaline phosphatase activity (McKay et al 1996, Fleury et al 2006).…”

Section: Osteoblastsmentioning

confidence: 99%

“…A decrease in protein, DNA, and RNA synthesis has been shown with Co and Cr ions (Hodges and Chipman 2002). Collagen type I synthesis is markedly reduced after exposure to Co and Cr particles or ions at concentrations of 10 µg/L or 100 µg/L (Wang et al 1997, Hallab et al 2001, Anissian et al 2002. Proteins from the extracellular matrix such as bone sialoprotein, osteocalcin, and osteopontin are important for calcification of the bone matrix (Robey and Boskey 2006).…”

Section: Osteoblastsmentioning

confidence: 99%

“…Co and Cr ions induce oxidation and nitration of proteins and dysregulation of the expression of antioxidant enzymes (Fleury et al 2006). The cells are capable of releasing proinflammatory cytokines into the microenvironment, such as IL-6 and TNF-α (Hallab et al 2001, Anissian et al 2002, Hallab et al 2002. These cytokines can in turn activate the differentiation of preosteoclasts into mature boneresorbing cells (Kudo et al 2003).…”

Section: Osteoblastsmentioning

confidence: 99%

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Metal-on-metal hip resurfacing arthroplasty: A review of periprosthetic biological reactions

et al. 2008

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“…In addition, Co and Cr are toxic to osteoblasts, leading to markedly reduced alkaline phosphatase activity (McKay et al, 1996;Fleury et al, 2006). The cells can also release pro-inflammatory cytokines such as IL-6 and TNF- (Hallab, 2001;Anissian et al, 2002;Hallab et al, 2002). These cytokines can in turn activate the differentiation of pre-osteoclasts into mature bone resorbing cells (Kudo et al, 2003).…”

Section: Metallurgymentioning

confidence: 99%

Hip resurfacing arthroplasty: current status and future perspectives

Corten

Ganz²,

Simon³

et al. 2011

eCM

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Hip resurfacing arthroplasty (HRA) is a concept of hip replacement that allows treating young active patients with a femoral bone preserving procedure. The proposed advantages of resuming an active lifestyle with increased frequency and duration of sports activities have been shown to be realistic. The 30-year cost-effectiveness in young male patients has been shown to be higher in resurfacing compared to conventional total hip replacement (THA). However, prognosticators of an inferior outcome have also been identified. The most important patient related factors are secondary osteoarthritis as the indication for surgery such as post-childhood hip disorders or AVN, female gender, smaller component sizes and older age (>65 years for males and >55 years for females). In addition, surgical technique (approach and cementing technique) and component design are also important determinant factors for the risk of failure. Moreover, concerns have surfaced with respect to high metal ion concentrations and metal ion hypersensitivities. In addition, the presumed ease of revising HRA has not reflected in improved or equal survivorship in comparison to a primary THA. This highlights the importance of identifying patient-, surgery-, and implant-related prognosticators for success or failure of HRA. Rather than vilifying the concept of hip resurfacing, detailed in depth analysis should be used to specify indications and improve implant design and surgical techniques.

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Cobalt ions influence proliferation and function of human osteoblast-like cells

Cited by 62 publications

References 19 publications

Cobalt ions induce chemokine secretion in primary human osteoblasts

Cobalt ions induce chemokine secretion in primary human osteoblasts

Metal-on-metal hip resurfacing arthroplasty: A review of periprosthetic biological reactions

Hip resurfacing arthroplasty: current status and future perspectives

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