Cobalt(III) complexes as potential anticancer agents: Physicochemical, structural, cytotoxic activity and DNA/protein interactions

Thamilarasan, Vijayan; Sengottuvelan, Nallathambi; Sudha, A.; Srinivasan, Pappu; Chakkaravarthi, G.

doi:10.1016/j.jphotobiol.2016.06.024

Cited by 58 publications

(20 citation statements)

References 65 publications

(68 reference statements)

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“…Currently, the development of metal complexes with nonplatinum central atoms including ruthenium [11], gold [12], silver [13], copper [14], zinc [15], Manganese [16], nickel [17], iridium [18] and cobalt [19] has been one of the hottest emerging areas of anticancer drug research. Among these, carbonyl cobalt complex has strong properties of cancer prevention including tumour growth/metastasis suppression [20], antiproliferative [21], DNA cleavage [22], apoptotic induction [23] and autophagy [8], which was more active than cisplatin on the human breast tumour cell lines (MCF-7 and MDA-MB-231) [24,25] and ovarian cancer cells (SKOV3/ DDP) [26]. With the concept of carbonyl metal CO-releasing molecules (CORMs) appearing, the anti-inflammatory effects were found through liberating CO to inhibit nitrite content [27].…”

Section: Introductionmentioning

confidence: 99%

Co-SLD suppressed the growth of oral squamous cell carcinoma via disrupting mitochondrial function

Zhang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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To evaluate the safety and efficacy of novel cobalt complex with sulindac (Co-SLD), the zebrafish and oral squamous cell carcinoma CAL27 were investigated in the present study. The developmental toxicity of Co-SLD ranging from 5 to 20 lM was determined by exposure to 3-144-h post-fertilization (hpf) zebrafish. Our data showed that Co-SLD did not cause to the appreciable toxicity at low concentration (5 and 10 lM). A remarkable toxicity was observed at high concentration (20 lM), including increased mortality and malformation, delayed hatchability, reduced heart rate as well as suppressed behaviour. With regard to the antitumor activity of Co-SLD, inhibited cell growth and migration capability were outstandingly observed in oral squamous cell carcinoma treated with 10 and 20 lM Co-SLD, which could be mainly attributed to the Co-SLD-elicited mitochondrial damage as marked by the depression of mitochondrial membrane potential, ROS accumulation and ATP depletion. Furthermore, administration of 10 lM Co-SLD was an optimal concentration not only to avoid the normal tissue toxicity, but also to enhance the killing of cancer cells via disrupting mitochondrial dysfunction. Taken together the above results demonstrated the desirable response of oral squamous cell carcinoma to Co-SLD. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Co-SLD suppressed the growth of oral squamous cell carcinoma via disrupting mitochondrial function

Zhang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…In our study, complex ( 2 ) effectively cleaved the DNA in a higher concentration. The same effect was obtained by Thamilarasan et al (2016) with a higher concentration of the complexes ([Co(acac)(bpy)(N 3 ) 2 ·H 2 O, ([Co(acac)(en)(N 3 ) 2 and ([Co(acac)(2-pic)(N 3 ) 2 , where acac = acetylacetone, bpy = 2,2′-bipyridine, en = ethylenediamine, 2-pic = picolylamine, and NaN 3 = sodium azide), and the cleavage was found to be much more efficient [ 55 ]. This suggested that the cleavage of pBR322 DNA depends on the concentration of the complexes.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Activity of Co(III) Complexes with Diamine Chelate Ligands against a Broad Spectrum of Bacteria with a DNA Interaction Mechanism

et al. 2021

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Cobalt coordination complexes are very attractive compounds for their therapeutic uses as antiviral, antibacterial, antifungal, antiparasitic, or antitumor agents. Two Co(III) complexes with diamine chelate ligands ([CoCl2(dap)2]Cl (1) and [CoCl2(en)2]Cl (2)) (where dap = 1,3-diaminopropane, en = ethylenediamine) were synthesized and characterized by elemental analysis, an ATR technique, and a scan method and sequentially tested against Gram-positive and Gram-negative bacteria. The minimum inhibitory concentration results revealed that anaerobic and microaerophilic bacteria were found to be the most sensitive; the serial passages assay presented insignificant increases in bacterial resistance to both compounds after 20 passages. The synergy assay showed a significant reduction in the MIC values of nalidixic acid when combined with Compounds (1) or (2). The assessment of cell damage by the complexes was performed using scanning electron microscopy, transmission electron microscopy, and confocal microscopy, which indicated cell membrane permeability, deformation, and altered cell morphology. DNA interaction studies of the Co(III) complexes with plasmid pBR322 using spectrophotometric titration methods revealed that the interaction between Complex (1) or (2) and DNA suggested an electrostatic and intercalative mode of binding, respectively. Furthermore, the DNA cleavage ability of compounds by agarose gel electrophoresis showed nuclease activity for both complexes. The results suggest that the effect of the tested compounds against bacteria can be complex.

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“…The agarose gel was prestained with 0.5 g/mL ethidium bromide (EtBr) and visualized under UV light and photographed for further analysis. The cleavage properties were determined based on the ability of compounds to convert the supercoiled form (Form I) into nicked (Form II) and linear form (Form III) [23].…”

Section: Dna Cleavage Assaymentioning

confidence: 99%

“…Cobalt involves itself in the regulation of DNA synthesis indirectly and in cobalt-dependent proteins which make it an essential biological element [21]. Cobalt(III) complexes have been widely investigated in coordination chemistry and biochemistry [22] owing to their therapeutic activities [23]. Copper and cobalt are bioessential metals in all living systems which show antifungal and antibacterial properties against several pathogenic fungi and bacteria depending on the reaction with the central DNA system [24].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Cobalt(III) and Copper(II) Complexes of 2-((E)-(6-Fluorobenzo[d]thiazol-2-ylimino) methyl)-4-chlorophenol: DNA Binding and Nuclease Studies—SOD and Antimicrobial Activities

Savithri

Kumar

Vivek³

et al. 2018

International Journal of Spectroscopy

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A bidentate (N- and O-) imine-based ligand(L1)and its metal complexes of types [CuII(L1)2](C1), [CuII(L1)(Phen)](C2), [CoIII(L1)2](C3), and [CoIII(L1)(Phen)](C4)(L1= 2-((E)-(6-fluorobenzo[d]thiazol-2-ylimino)methyl)-4-chlorophenol and phen = 1,10-phenanthroline) were synthesized as potential chemotherapeutic drug candidates. The prepared complexes were structurally characterized by spectral techniques (NMR, FT-IR, LC-MS, EPR, and electronic absorption), thermogravimetric analysis (TGA/DTA), magnetic moment, and CHNO elemental analysis. Spectroscopic studies suggested the distorted octahedral structure for all complexes.In vitrobioassay studies include binding and nuclease activities of the ligand and its complexes with target calf thymus- (CT-) DNA were carried out by employing UV-Vis, fluorescence spectroscopy, viscosity, and gel electrophoresis techniques. The extent of binding propensity was determined quantitatively byKbandKsvvalues which revealed a higher binding affinity for C2and C4as compared to C1and C3. In addition, the scavenging superoxide anion free radical (O∙-2) activity of metal complexes was determined by nitroblue tetrazolium (NBT) light reduction assay. Molecular docking studies with DNA and SOD enzyme were also carried out on these compounds. The antimicrobial study has shown that all the compounds are potential antibacterial agents against Gram-negative bacterial strains and better antifungal agents with respect to standard drugs used.

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Cobalt(III) complexes as potential anticancer agents: Physicochemical, structural, cytotoxic activity and DNA/protein interactions

Cited by 58 publications

References 65 publications

Co-SLD suppressed the growth of oral squamous cell carcinoma via disrupting mitochondrial function

Co-SLD suppressed the growth of oral squamous cell carcinoma via disrupting mitochondrial function

Antibacterial Activity of Co(III) Complexes with Diamine Chelate Ligands against a Broad Spectrum of Bacteria with a DNA Interaction Mechanism

Synthesis and Characterization of Cobalt(III) and Copper(II) Complexes of 2-((E)-(6-Fluorobenzo[d]thiazol-2-ylimino) methyl)-4-chlorophenol: DNA Binding and Nuclease Studies—SOD and Antimicrobial Activities

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