Cobalt-chromium-enriched medium ameliorates shear-stressed endothelial cell performance

Machado, Mariana Issler Pinheiro; Gomes, Anderson Moreira; Ferreira, Marcel Rodrigues; Pinto, Tatiana Andrea Soares; Fernandes, Célio Júnior da Costa; Bezerra, Fábio; Zambuzzi, Willian Fernando

doi:10.1016/j.jtemb.2019.04.012

Cited by 18 publications

(12 citation statements)

References 44 publications

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“…was reached to determine which studies fully fulfilled the selection criteria. Then, 17 papers were excluded [ 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ], resulting in the inclusion of 21 individual studies ( Table 1 , Figure 1 ).…”

Section: Resultsmentioning

confidence: 99%

Toxicological Risks of the Cobalt–Chromium Alloys in Dentistry: A Systematic Review

et al. 2022

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Background: The toxicological risk of Co-Cr dental alloys is actually a sensitive subject with the European regulatory changes, namely regulation (EU) 2017/745 and annex VI to the CLP regulation (EC) 1972/2008. Objectives: The objective of this review is to conduct a rigorous analysis of the cytocompatibility of cobalt–chromium (Co-Cr) dental alloys. Considering various parameters such as cytotoxicity, type IV hypersensitivity reaction, sensitization, and irritation, we investigated evidence of toxicity of Co-Cr in human dental applications. Data sources: Specific search strategies were performed in three electronic databases, namely Medline, Embase, and Web of Science, using a main restriction in the search regarding the publication date (1995–2022). Study selection: Out of a total of 836 articles, only 21 studies were selected and analyzed according to PRISMA methodology. Results: Among them, 10 in vitro studies using human samples and 11 in vivo studies on human patients were distinguished. Most of the in vitro studies confirmed that Co-Cr alloys have a good cytocompatibility compared to Ni alloys. Regarding the in vivo studies, it appeared that Co-Cr could rarely cause sensitization, irritation, and allergic reactions. Reactions were mainly observed for people allergic to Co or Cr. Nevertheless, titanium-based materials showed better results. Conclusions: This study proposes a new state of the art on Co-Cr dental alloys and will thus be very useful for carrying out additional studies. Relevance: This review will help practitioners in their daily clinical choice.

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Section: Resultsmentioning

confidence: 99%

Toxicological Risks of the Cobalt–Chromium Alloys in Dentistry: A Systematic Review

et al. 2022

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“…Adipocyte-related conditioned medium was later used to incubate both types of titanium disc for 24 h: DAE and nHA, in accordance with ISO 10993:2016 (0.2 g/mL w / v ) with slight modification as suggested by Zambuzzi et al [ 23 , 24 , 25 , 26 ]. The final conditioned medium was later used to expose ECs for 3 days under shear stress to mimic blood flow.…”

Section: Methodsmentioning

confidence: 99%

Adipogenesis-Related Metabolic Condition Affects Shear-Stressed Endothelial Cells Activity Responding to Titanium

Pinto

Gomes

Morais

et al. 2023

JFB

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Purpose: Obesity has increased around the world. Obese individuals need to be better assisted, with special attention given to dental and medical specialties. Among obesity-related complications, the osseointegration of dental implants has raised concerns. This mechanism depends on healthy angiogenesis surrounding the implanted devices. As an experimental analysis able to mimic this issue is currently lacking, we address this issue by proposing an in vitro high-adipogenesis model using differentiated adipocytes to further investigate their endocrine and synergic effect in endothelial cells responding to titanium. Materials and methods: Firstly, adipocytes (3T3-L1 cell line) were differentiated under two experimental conditions: Ctrl (normal glucose concentration) and High-Glucose Medium (50 mM of glucose), which was validated using Oil Red O Staining and inflammatory markers gene expression by qPCR. Further, the adipocyte-conditioned medium was enriched by two types of titanium-related surfaces: Dual Acid-Etching (DAE) and Nano-Hydroxyapatite blasted surfaces (nHA) for up to 24 h. Finally, the endothelial cells (ECs) were exposed in those conditioned media under shear stress mimicking blood flow. Important genes related to angiogenesis were then evaluated by using RT-qPCR and Western blot. Results: Firstly, the high-adipogenicity model using 3T3-L1 adipocytes was validated presenting an increase in the oxidative stress markers, concomitantly with an increase in intracellular fat droplets, pro-inflammatory-related gene expressions, and also the ECM remodeling, as well as modulating mitogen-activated protein kinases (MAPKs). Additionally, Src was evaluated by Western blot, and its modulation can be related to EC survival signaling. Conclusion: Our study provides an experimental model of high adipogenesis in vitro by establishing a pro-inflammatory environment and intracellular fat droplets. Additionally, the efficacy of this model to evaluate the EC response to titanium-enriched mediums under adipogenicity-related metabolic conditions was analyzed, revealing significant interference with EC performance. Altogether, these data gather valuable findings on understanding the reasons for the higher percentage of implant failures in obese individuals.

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“…To prepare the titanium-enriched medium, both dual acid-etching (DAE) treating surface (named W/DAE) and the nanohydroxyapatite-blasted surfaces (named HAnano®) were incubated in cell culture media (RPMI) without FBS up to 24 h at 37°C, 5% CO 2 , and 95% humidity (0.2 g/mL ( w / v ); ISO 10993:2016), as we have proposed earlier to evaluate biomaterials [ 11 , 29 , 30 ]. This is expected that the conditioned medium contains molecules released from those metallic alloys and might affect the biology of endothelial cells.…”

Section: Methodsmentioning

confidence: 99%

Nanohydroxyapatite-Blasted Bioactive Surface Drives Shear-Stressed Endothelial Cell Growth and Angiogenesis

Pinto

Martins

Ferreira

et al. 2022

BioMed Research International

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Nanosized crystalline hydroxyapatite coating (HAnano®) accelerates the osteointegration of dental implants which is hypothesized to drive angiogenesis. In order to test this hypothesis, we have subjected shear-stressed human umbilical vein endothelial cells (HUVECs) to a HAnano®-enriched medium, as well as to surface presenting dual acid etching (DAE) as a control. To note, the titanium implants were coated with 10 nm in diameter HA particles using the Promimic HAnano method. Our data reveals that HAnano® modulates higher expression of genes related with endothelial cell performance and viability, such as VEGF, eNOS, and AKT, and further angiogenesis in vitro by promoting endothelial cell migration. Additionally, the data shows a significant extracellular matrix (ECM) remodeling, and this finding seems developing a dual role in promoting the expression of VEGF and control endothelial cell growth during angiogenesis. Altogether, these data prompted us to further validate this phenomenon by exploring genes related with the control of cell cycle and in fact our data shows that HAnano® promotes higher expression of CDK4 gene, while p21 and p15 genes (suppressor genes) were significantly lower. In conjunction, our data shows for the first time that HAnano®-coated surfaces drive angiogenesis by stimulating a proliferative and migration phenotype of endothelial cells, and this finding opens novel comprehension about osseointegration mechanism considering nanosized hydroxyapatite coating dental implants.

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Cobalt-chromium-enriched medium ameliorates shear-stressed endothelial cell performance

Cited by 18 publications

References 44 publications

Toxicological Risks of the Cobalt–Chromium Alloys in Dentistry: A Systematic Review

Toxicological Risks of the Cobalt–Chromium Alloys in Dentistry: A Systematic Review

Adipogenesis-Related Metabolic Condition Affects Shear-Stressed Endothelial Cells Activity Responding to Titanium

Nanohydroxyapatite-Blasted Bioactive Surface Drives Shear-Stressed Endothelial Cell Growth and Angiogenesis

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