The paper presents results of investigations of zinc and manganese concentration in the water, bottom deposits and ichthyofauna of Yavoriv water reservoir, which was formed in 2002 in frames of project of Yavoriv sulfur quarry revitalization. Mentioned elements are characterized by wide spectrum of action in fish body, which, depending on their concentration, can be either physiologic or toxic. It was revealed, that concentration of Zn and Mn in the water of Yavoriv water reservoir varied considerably depending on place of samples taking. The lowest Zn concentration in the water was below 1 MPL (maximum permitted level), namely 0.6 µg/l, and the highest – more than 7 MPL (70.5 µg/l). Similar differences were observed concerning Mn concentration in the water – the range of determined values varied from 3.3 (0.3 MPL) to 48.6 µg/l. The same peculiarities of Zn and Mn distribution were estimated in regard to their content in bottom deposits. In particular, difference of Zn concentration in samples of bottom deposits was near 23 times – from 5.4 to 230.0 mg/kg depending on the point of samples taking. Mn content in bottom deposits varies from 61.5 to 1500 mg/kg. Zn and Mn concentration in skeletal muscles, gills and skin of perch and rudd didn’t exceed MPL regardless their content in the water and bottom deposits. In most of cases, gills accumulated these elements more than other investigated tissues.