Coaxial nanofibers of chitosan–alginate–PEO polycomplex obtained by electrospinning

Nista, Silvia Vaz Guerra; Bettini, Jefferson; Mei, Lucía Helena Innocentini

doi:10.1016/j.carbpol.2015.03.063

Cited by 88 publications

(42 citation statements)

References 21 publications

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“…Large‐scale cells encapsulation and bulk production of the microfibers remain a bottleneck. Core–shell nanofibers have been obtained by electrospinning the blends of NaA/PEO and CS/PEO; however, cell encapsulation and culture in the solid nanofibers are still issues . In addition, those methods could not manipulate the microfluid in a controlled manner and thus could not control the structure of the microfiber and its inner environment, which are critical for the cell culture and tissue engineering application.…”

Section: Introductionmentioning

confidence: 99%

Simple fabrication of inner chitosan‐coated alginate hollow microfiber with higher stability

Liu

Wang

et al. 2019

J Biomed Mater Res

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Sodium alginate (NaA) has been widely used as microfiber‐based scaffold material. However, Ca‐alginate microfiber might disintegrate in the physiological environment due to the loss of calcium ions, which will limit its long‐term application in tissue engineering. In this work, to enhance the stability of Ca‐alginate microfiber in the physiological environment, an inner chitosan coating was introduced to Ca‐alginate hollow microfiber by one step via a microfluidic device. A more stable composite microfiber with double cross‐linking layers was generated. The stability of the microfiber was studied in the PBS solution (pH 7.4) to identify the coating effect on the hollow structure. The results revealed that chitosan component bonded an NaA layer to form a stable polyelectrolyte complex membrane in the inner wall of the microfiber, which stabilized the hollow region even though the Ca‐alginate shell was disintegrated by PBS solution. In addition, the introduction of chitosan coating improved the inner environment of the low affinity of alginate to cell surfaces and facilitated the cell adhesion and culture in the microfiber. HepG2 cells in the microfibers displayed favorable cell viability and proliferation ability. We believe that this work will lead to the development of innovative methodologies and materials for both cell culture and tissue engineering application. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2527–2536, 2019.

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Section: Introductionmentioning

confidence: 99%

Simple fabrication of inner chitosan‐coated alginate hollow microfiber with higher stability

Liu

Wang

et al. 2019

J Biomed Mater Res

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“…This technique is able to simultaneously electrospun different polymers obtaining core–shell structure nanofibers . Several publications can be found related to co‐electrospun nanofibers based on different polymers, such as, PEG or PLA , block copolymers and based on hybrid nanofibers with chitosan , cellulose , multiwalled carbon nanotubes (MWCNTs) , or inorganic nanoparticles .…”

Section: Introductionmentioning

confidence: 99%

Optimization of the electrospinning processing‐window to fabricate nanostructured PE‐b‐PEO and hybrid PE‐b‐PEO/EBBA fibers

Carrasco‐Hernandez

Gutierrez

Peponi

et al. 2017

Polymer Engineering & Sci

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An electrospinning technique is used to obtain poly(ethylene-b-ethylene oxide) block copolymer (PE-b-PEO) fibers. Optimization of the electrospinning processing-window is carried out by varying the concentration of the block copolymer solution and playing with three different electrospinning parameters, applied voltage, solvent and block copolymer solution flow rate. The influence of the concentration of the block copolymer solution over the length and diameter of the fibers is mainly studied and results indicate that the optimum conditions to fabricate PE-b-PEO block copolymer fibers are concentrations between 45 and 47 wt% of PE-b-PEO block copolymer and chloroform/DMF mixture ratio of 4:1 and 5:1. Moreover, low solvent and block copolymer solution flow rates led to the longest and widest fibers. Hybrid fibers are also fabricated modifying PE-b-PEO block copolymer fibers with low molecular weight N-(4-ethoxybenzylidene)24butylaniline (EBBA) nematic liquid crystal using coaxial electrospinning technique. The morphology of the fabricated fibers is investigated from micro to nanoscale. Atomic force microscopy (AFM) results show selforganization of investigated PE-b-PEO and hybrid PE-b-PEO/EBBA fibers on the nanometric scale, which make them interesting from the point of view of novel applications as template materials.

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“…In recent years, our research group has been dedicated to the study of nanostructured biopolymer Silver nanoparticles incorporated into nanostructured biopolymer membranes produced by electrospinning 913 membranes for application in the medical and dental fields (Nista et al, 2012;Nista et al, 2013;Nista, Bettini, Mei, 2015;Bizarria, Davila, Mei, 2014;Kleinubing et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies have shown that an especially lucrative opportunity lies in applying these polymeric nanostructures in the medical field (Abdelgawada, Hudsona, Rojas, 2014;Chaloupka, Malam, Seifalian, 2010;Dallas, Sharma, Zboril, 2011). The literature contains a great deal of studies demonstrating the successful application of these nanostructures in the healthcare, pharmaceutical and cosmetic industries for repairing and regenerating the skin and organs; as drug delivery vectors and therapies; biocompatible and biodegradable implants; in medical diagnosis and instrumentation; as tissue protective agents that guard against infection; and in cosmetics, molecular medicines, supplements and body care applications in the field of dentistry (Nista, Bettini;Mei, 2015;Bizarria, Davila, Mei, 2014;Kleinubing et al, 2014).The AgNPs possess superior antimicrobial properties compared to other salts, due to their extremely large surface area, which provides better contact with microorganisms (Rai, Yadav, Gade 2009). Although the mechanism of action of silver nanoparticles on microorganisms is not fully understood, there are hypotheses that these nanoparticles can cause cell lysis or inhibit cell transduction (Prabhu, Poulose, 2012).…”

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confidence: 99%

“…The authors showed that the polymeric solution (PVA/GK/Ag) and the final AgNP-containing membrane (Ag-MEM) exhibited clear antibacterial activity toward Gram-negative E. coli and P. aeruginosa and Gram-positive S. aureus. These newly synthesized nanomaterials show great potential for the development of environmentally friendly antibacterial devices for medical, food packaging, and water purification purposes (Padil et al, 2015).In recent years, our research group has been dedicated to the study of nanostructured biopolymer Silver nanoparticles incorporated into nanostructured biopolymer membranes produced by electrospinning 913 membranes for application in the medical and dental fields (Nista et al, 2012;Nista et al, 2013;Nista, Bettini, Mei, 2015;Bizarria, Davila, Mei, 2014;Kleinubing et al, 2014).This work focuses on the synthesis, characterization, and comparative antimicrobial activities of AgNPs incorporated into nanostructured biopolymer membranes made from either a blend of CTS/PEO or CA when tested against both Gram-positive and Gram-negative bacteria. …”

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confidence: 99%

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Silver nanoparticles incorporated into nanostructured biopolymer membranes produced by electrospinning: a study of antimicrobial activity

Segala

Nista

Cordi

et al. 2015

Braz. J. Pharm. Sci.

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This study examines the antimicrobial activity of silver nanoparticles incorporated into nanostructured membranes made of cellulose acetate (CA) and blends of chitosan/poly-(ethylene oxide, CTS/PEO) and prepared by electrospinning. The formation of chemically synthesized Ag nanoparticles (AgNPs) was monitored by UV-visible spectroscopy (UV-Vis) and characterized by transmission electron microscopy (TEM). The size distribution of the AgNPs was measured by dynamic light scattering (DLS), with an average size of approximately 20 nm. The presence of AgNPs on the surface of electrospun nanofibers was observed by field emission electron microscopy (FEG) and confirmed by TEM. The antimicrobial activity of AgNPs incorporated into nanostructured membranes made of CA and CTS/PEO electrospun nanofibers was evaluated in the presence of both Gram-positive bacteria, such as Staphylococcus aureus ATCC 29213 and Propionibacterium acnes ATCC 6919, and Gram-negative bacteria, such as Escherichia coli ATCC 25992 and Pseudomonas aeruginosa ATCC 17933. Microbiological results showed that the presence of AgNPs in CA and CTS/PEO nanostructured membranes has significant antimicrobial activity for the Gram-positive bacteria Escherichia coli and Propionibacterium acnes. Uniterms:Electrospinning. Silver nanoparticles/antibacterial activity. Cellulose acetate. Chitosan.Neste trabalho avaliou-se a atividade antimicrobiana das nanopartículas de prata (AgNPs) incorporadas em membranas de acetato celulose (AC) e blendas de quitosana/poli-óxido de etileno (CTS/PEO) preparadas pelo método de eletrofiação. A formação das AgNPs previamente sintetizadas foi monitorada por UV-Vis e caracterizada por microscopia eletrônica de transmissão (MET). A distribuição de tamanho das AgNPs foi mensurada por espalhamento de luz dinâmico, com tamanho médio em torno de 20 nm. A presença das NPs na superfície das nanofibras eletrofiadas foi observada por microscopia eletrônica com emissão de campo (FEG) e confirmada por MET. A atividade antimicrobiana das membranas nanoestruturadas de AC e CTS/PEO foi avaliada pelo uso de bactérias Gram-positivas, tais como Staphylococcus aureus ATCC 29213 e Propionibacterium acnes ATCC 6919, e Gram-negativas, como Escherichia coli ATCC 25992 e Pseudomonas aeruginosa ATCC 17933. Os resultados microbiológicos mostraram a presença das AgNPs nas membranas de AC e CTS/PEO com significativa atividade antimicrobiana para Escherichia coli e Propionibacterium acnes, respectivamente.Unitermos: Eletrofiação. Nanopartículas de prata/atividade antimicrobiana. Acetato de celulose. Quitosana. 912 INTRODUCTIONThe use of metal nanoparticles in various medical and biotechnological applications is one of the most investigated areas in materials science. These applications require appropriate chemical functionalization of the nanoparticles with organic molecules or their incorporation into polymer matrices (Dallas, Sharma, Zboril, 2011). Among the numerous types of nanoparticles that have been used to decorate polymers, silver nanoparticles (AgNPs) ...

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Coaxial nanofibers of chitosan–alginate–PEO polycomplex obtained by electrospinning

Cited by 88 publications

References 21 publications

Simple fabrication of inner chitosan‐coated alginate hollow microfiber with higher stability

Simple fabrication of inner chitosan‐coated alginate hollow microfiber with higher stability

Optimization of the electrospinning processing‐window to fabricate nanostructured PE‐b‐PEO and hybrid PE‐b‐PEO/EBBA fibers

Silver nanoparticles incorporated into nanostructured biopolymer membranes produced by electrospinning: a study of antimicrobial activity

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