Coaxial bioprinting vascular constructs: A review

Xu, Huilun; Su, Yimeng; Liao, Ziming; Liu, Zhengjiang; Huang, Xiaobo; Zhao, Liqin; Duan, Ruxin; Hu, Yinchun; Yan, Wei; Lian, Xiaojie; Huang, Di

doi:10.1016/j.eurpolymj.2022.111549

Cited by 9 publications

(6 citation statements)

References 114 publications

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“…The use of naturally derived biomaterials such as alginate, collagen, GelMA, and chitosan promotes favorable cellular interactions within the organoid microenvironment. 193 By optimizing parameters such as nozzle configuration, bioink viscosity, and extrusion rate, coaxial bioprinting can successfully generate vessels tailored to the specific diffusion and perfusion requirements of individual organoid types. 193 , 194 , 195 , 196 …”

Section: Methods For Engineering Vascularized Organoidsmentioning

confidence: 99%

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Bioengineering methods for vascularizing organoids

Nwokoye,

Abilez

2024

Cell Reports Methods

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Section: Methods For Engineering Vascularized Organoidsmentioning

confidence: 99%

“… 193 By optimizing parameters such as nozzle configuration, bioink viscosity, and extrusion rate, coaxial bioprinting can successfully generate vessels tailored to the specific diffusion and perfusion requirements of individual organoid types. 193 , 194 , 195 , 196 …”

Section: Methods For Engineering Vascularized Organoidsmentioning

confidence: 99%

Bioengineering methods for vascularizing organoids

Nwokoye,

Abilez

2024

Cell Reports Methods

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“…It leverages the concentric extrusion of multiple biomaterials through coaxial nozzles, enabling the simultaneous deposition of core (bioink) and shell (crosslinker) materials ( 249 ). Natural biomaterials, such as alginate, collagen, GelMA, and chitosan, are frequently used as coaxial bioinks and have superior biocompatibility relative to synthetic materials ( 250 ). Coaxial bioprinting enables the fabrication of intricate constructs, solid or hollow tubes, in a single step.…”

Section: Evolution Of Vascularized Tissues and Organoidsmentioning

confidence: 99%

“…For instance, in creating hollow tubes that mimic tubular vascular structures, the crosslinker is extruded from the inner nozzle, while the bioink is dispensed from the outer nozzle ( 251 ). Several parameters affect the bioprinting outcome, including nozzle diameter, bioink viscosity, and printing extrusion rate ( 250 ). Therefore, these factors must be carefully considered to achieve optimal results.…”

Section: Evolution Of Vascularized Tissues and Organoidsmentioning

confidence: 99%

Blood vessels in a dish: the evolution, challenges, and potential of vascularized tissues and organoids

Nwokoye,

Abilez

2024

Front. Cardiovasc. Med.

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Vascular pathologies are prevalent in a broad spectrum of diseases, necessitating a deeper understanding of vascular biology, particularly in overcoming the oxygen and nutrient diffusion limit in tissue constructs. The evolution of vascularized tissues signifies a convergence of multiple scientific disciplines, encompassing the differentiation of human pluripotent stem cells (hPSCs) into vascular cells, the development of advanced three-dimensional (3D) bioprinting techniques, and the refinement of bioinks. These technologies are instrumental in creating intricate vascular networks essential for tissue viability, especially in thick, complex constructs. This review provides broad perspectives on the past, current state, and advancements in key areas, including the differentiation of hPSCs into specific vascular lineages, the potential and challenges of 3D bioprinting methods, and the role of innovative bioinks mimicking the native extracellular matrix. We also explore the integration of biophysical cues in vascularized tissues in vitro, highlighting their importance in stimulating vessel maturation and functionality. In this review, we aim to synthesize these diverse yet interconnected domains, offering a broad, multidisciplinary perspective on tissue vascularization. Advancements in this field will help address the global organ shortage and transform patient care.

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“…[8,9] For small-diameter artificial vessels, the printing resolution has been a challenge that has plagued this research. [10] There are various methods to print small-diameter artificial vessels, but each method has a limited minimum print resolution. [11,12] Various instruments or materials have been applied to improve the printing resolution in experiments.…”

Section: Introductionmentioning

confidence: 99%

Complexation‐Induced Resolution Enhancement Pleiotropic Small Diameter Vascular Constructs with Superior Antibacterial and Angiogenesis Properties

Liu

Yan

et al. 2023

Adv Healthcare Materials

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Abstract3D printing has been widely applied for preparing artificial blood vessels, which will bring innovation to cardiovascular disorder intervention. However, the printing resolution and anti‐infection properties of small‐diameter vessels (Φ < 6 mm) have been challenging in 3D printing. The primary objective of this research is to design a noval coaxial 3D printing post‐processing method for preparing small‐size blood vessels with improved antibacterial and angiogenesis properties. The coaxial printing resolution can be more conveniently improved. Negatively charged polyvinyl alcohol (PVA) and alginate (Alg) interpenetrating networks artificial vessels are immersed in positively charged chitosan (CTS) solution. Rapid dimensional shrinkage takes place on its outer surface through electrostatic interactions. The maximum shrinkage size of wall thickness can reach 61.2%. The vessels demonstrate strong antibacterial properties against Escherichia coli (98.8 ± 0.5%) and Staphylococcus aureus (97.6 ± 1.4%). In rat dorsal skin grafting experiments, Cu2+ can promote angiogenesis by regulating hypoxia‐inducible factor‐1 pathway. No artificial blood vessel blockage occur after 5 d of blood circulation in vitro.This article is protected by copyright. All rights reserved

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Coaxial bioprinting vascular constructs: A review

Cited by 9 publications

References 114 publications

Bioengineering methods for vascularizing organoids

Bioengineering methods for vascularizing organoids

Blood vessels in a dish: the evolution, challenges, and potential of vascularized tissues and organoids

Complexation‐Induced Resolution Enhancement Pleiotropic Small Diameter Vascular Constructs with Superior Antibacterial and Angiogenesis Properties

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