Coating typologies and constrained swelling of hyaluronic acid gels within scaffold pores

Abstract

A set of elastomeric scaffolds with a well defined porous structure was prepared with a template leaching procedure and coated with hyaluronic acid solutions. Depending on the coating process parameters the hyaluronic acid deposited on the pores had configurations ranging from thin disconnected aggregates to a thick continuous layer on the pore surface. The development of the coating layer was studied by scanning electron microscopy and the materials were subjected to dynamical and equilibrium swelling experiments in a water vapor ambient of fixed activity. The porosity change due to coating and to swelling of the coating layer were determined. The hyaluronic acid coating the pores has a different swelling capacity depending on the type of layer formed, as a consequence of the scaffold constraint and of the layer typology. These factors were investigated analytically by modifying the standard theory of gel swelling. An experimental quantity is introduced which reflects the constrainment build-up on gel swelling.

Electrospun adherent–antiadherent bilayered membranes based on cross-linked hyaluronic acid for advanced tissue engineering applications

“…In [15] divinylsulfone (DVS) was proposed as two step crosslinker for HA films, and in [16] it was successfully employed by the authors to crosslink HA coatings of acrylate scaffolds' pores in a two step process. In this work, unmodified HA has been successfully crosslinked with said crosslinker 3 for the first time in one single step during the process of electrodeposition and with no additional post-treatments.…”

Development of Bioactive Patch for Maintenance of Implanted Cells at the Myocardial Infarcted Site

“…Poly(ethyl acrylate) (PEA) scaffolds with interconnected cylindrical orthogonal pores were obtained by the template leaching method described before [47][48][49]. On the other hand, poly(caprolactone 2-(methacryloyloxy)ethyl ester) (PCLMA) scaffolds with spherical interconnected pores were obtained by a procedure analogous to that followed by Escobar Ivirico et al [34].…”

Design and Assembly Procedures for Large-Sized Biohybrid Scaffolds as Patches for Myocardial Infarct

“…In a second series of scaffolds (hereafter referred to as 2-step loaded, Fig. 1b), the SAP was incorporated as a 0.15% (w/v) solution with the help of some vacuum, as in 19,39 ; more precisely, the 8-mm scaffold discs were placed (folded if necessary) in a 50-mL sterile syringe, then the aqueous SAP solution was loaded, and the air removed. Next, maintaining the Luer taper of the sealed syringe, the peptide solution was forced to penetrate throughout the scaffold by repeatedly pulling the syringe plunger until the samples were completely wet.…”