Coating of titanium implants with collagen, RGD peptide and chondroitin sulfate

Rammelt, Stefan; Illert, Till; Bierbaum, Susanne; Scharnweber, Dieter; Zwipp, H.; Schneiders, Wolfgang

doi:10.1016/j.biomaterials.2006.06.034

Cited by 268 publications

(240 citation statements)

References 47 publications

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“…Interaction of hMSCs with Col I occurs via ² 1 integrins and has been shown to be critical for their osteogenic differentiation when cultured on Col I matrix including phosphorylation of focal adhesion kinase, downstream signaling by MAP kinase related to expression of osteogenic transcription factors [58,59]. On the other hand, many studies demonstrated the beneficial of CS on bone formation [60,61]. Murphy and the co-workers found that the type of GAG used, can control the lineage specification of MSCs, whereby HA enhanced chondrogenic differentiation and CS promoted osteogenic MSC differentiation [61].…”

Section: Osteogenic Differentiation Of Hadscsmentioning

confidence: 99%

Molecular composition of GAG-collagen I multilayers affects remodeling of terminal layers and osteogenic differentiation of adipose-derived stem cells

et al. 2016

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T. (2016) Molecular composition of GAG-collagen I multilayers affects remodeling of terminal layers and osteogenic differentiation of adipose-derived stem cells. Acta Biomaterialia, 41, pp. 86-99. (doi:10.1016Biomaterialia, 41, pp. 86-99. (doi:10. /j.actbio.2016 This is the author's final accepted version.There may be differences between this version and the published version. You are advised to consult the publisher's version if you wish to cite from it.http://eprints.gla.ac.uk/119626/ AbstractThe effect of molecular composition of multilayers, by pairing type I collagen (Col I) with either hyaluronic acid (HA) or chondroitin sulfate (CS) was studied regarding the osteogenic differentiation of adhering human adipose-derived stem cells (hADSCs). Multilayer (PEM)formation was based primarily on ion pairing and on additional intrinsic cross-linking through imine bond formation replacing native by oxidized HA (oHA) or CS (oCS) with Col I.Significant amounts of Col I fibrils were found on both native and oxidized CS-based PEMs, resulting in higher water contact angles and surface potential under physiological condition, while much less organized Col I was detected in either HA-based multilayers, which were more hydrophilic and negatively charged. An important finding was that hADSCs remodeled Col I at the terminal layers of PEMs by mechanical reorganization and pericellular proteolytic degradation, being more pronounced on CS-based PEMs. This was in accordance with the higher quantity of Col I deposition in this system, accompanied by more cell spreading, focal adhesions (FA) formation and significant α2β1 integrin recruitment compared to HA-based PEMs. Both CS-based PEMs caused also an increased fibronectin (FN) secretion and cell growth. Furthermore, significant calcium phosphate deposition, enhanced ALP, Col I and Runx2 expression were observed in hADSCs on CS-based PEMs, particularly on oCS-containing one. Overall, multilayer composition can be used to direct cell-matrix interactions, and hence stem cell fates showing for the first time that PEMs made of biogenic polyelectrolytes undergo significant remodeling of terminal protein layers, which enables cells to form a more adequate extracellular matrix-like environment.

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Section: Osteogenic Differentiation Of Hadscsmentioning

confidence: 99%

Molecular composition of GAG-collagen I multilayers affects remodeling of terminal layers and osteogenic differentiation of adipose-derived stem cells

et al. 2016

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“…Recent studies concerning collagen coatings on Ti hard-tissue implants have demonstrated their effective roles in stimulating cellular responses [11], increasing bone remodeling [12,13], and improving bone growth and bone-implant contact [14]. Therefore, hybrids of collagen and HA have greater potential for clinical applications than their pure HA or collagen equivalents, because they benefit from the advantageous properties of both materials, while retaining a similar composition and structure to that of human hard tissues.…”

Section: Introductionmentioning

confidence: 99%

Bioactive nanocomposite coatings of collagen/hydroxyapatite on titanium substrates

Teng

Lee

Park

et al. 2008

J Mater Sci: Mater Med

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Collagen/hydroxyapatite (HA) nanocomposite thin films containing 10, 20, and 30 wt.% HA were prepared on commercially pure titanium substrates by the spin coating of their homogeneous sols. All of the nanocomposite coatings having a thickness of *7.5 lm exhibited a uniform and dense surface, without any obvious aggregation of the HA particles. A minimum contact angle of 36.5°w as obtained at 20 wt.% HA, suggesting that these coatings would exhibit the best hydrophilicity. The in vitro cellular assays revealed that the coating treatment of the Ti substrates favored the adhesion of osteoblast-like cells and significantly enhanced the cell proliferation rate. The cells on the nanocomposite coatings expressed much higher alkaline phosphatase (ALP) levels than those on the uncoated Ti substrates. Increasing the amount of HA resulted in a gradual improvement in the ALP activity. The nanocomposite coatings on Ti substrates also exhibited much better cell proliferation behaviors and osteogenic potentials than the conventional composite coatings with equivalent compositions, demonstrating the greater potential of the former as implant materials for hard tissue engineering.

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“…To compensate for this, the spacer has to be adapted. Though this immobilization system was successfully tested in animal models [4,23] it still shows limitations. The number of different molecules immobilizable at one time is limited.…”

Section: Resultsmentioning

confidence: 99%