Coating of Polyetheretherketone Films with Silver Nanoparticles by a Simple Chemical Reduction Method and Their Antibacterial Activity

Cruz-Pacheco, Andrés F.; Muñoz-Castiblanco, Tatiana; Cuaspud, Jairo Alberto Gómez; Paredes-Madrid, Leonel; Vargas, Carlos Arturo Parra; Martínez, José Javier Pérez-Fadón; Gómez, Carlos Andrés Palacio

doi:10.3390/coatings9020091

Cited by 20 publications

(14 citation statements)

References 66 publications

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“…The formation of a silver layer on a glass surface has been well-known for several decades and described in multiple studies [ 39 , 40 , 41 , 42 , 43 , 44 ] and patents [ 45 , 46 , 47 ] by applying various techniques. The most-common silver-deposition reaction is based on Tollens’ reagent, which is a colorless solution of a diamminesilver(I) complex that is created by a mixture of silver nitrate, ammonia, and alkaline [ 48 , 49 , 50 , 51 , 52 , 53 ]. The silver-layer formation is highly influenced by the various conditions of the deposition procedure.…”

Section: Resultsmentioning

confidence: 99%

The Incorporation of Amplified Metal-Enhanced Fluorescence in a CMOS-Based Biosensor Increased the Detection Sensitivity of a DNA Marker of the Pathogenic Fungus Colletotrichum gloeosporioides

2020

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Half of the global agricultural fresh produce is lost, mainly because of rots that are caused by various pathogenic fungi. In this study, a complementary metal-oxide-semiconductor (CMOS)-based biosensor was developed, which integrates specific DNA strands that allow the detection of enoyl-CoA-hydratase/isomerase, which is a quiescent marker of Colletotrichum gloeosporioides fungi. The developed biosensor mechanism is based on the metal-enhanced fluorescence (MEF) phenomenon, which is amplified by depositing silver onto a glass surface. A surface DNA strand is then immobilized on the surface, and in the presence of the target mRNA within the sample, the reporter DNA strand that is linked to horseradish peroxidase (HRP) enzyme will also bind to it. The light signal that is later produced from the HRP enzyme and its substrate is enhanced and detected by the coupled CMOS sensor. Several parameters that affect the silver-deposition procedure were examined, including silver solution temperature and volume, heating mode, and the tank material. Moreover, the effect of blocking treatment (skim milk or bovine serum albumin (BSA)) on the silver-layer stability and nonspecific DNA absorption was tested. Most importantly, the effect of the deposition reaction duration on the silver-layer formation and the MEF amplification was also investigated. In the study findings a preferred silver-deposition reaction duration was identified as 5–8 min, which increased the deposition of silver on the glass surface up to 13-times, and also resulted in the amplification of the MEF phenomenon with a maximum light signal of 50 relative light units (RLU). It was found that MEF can be amplified by a customized silver-deposition procedure that results in increased detection sensitivity. The implementation of the improved conditions increased the biosensor sensitivity to 3.3 nM (4500 RLU) with a higher detected light signal as compared to the initial protocol (400 RLU). Moreover, the light signal was amplified 18.75-, 11.11-, 5.5-, 11.25-, and 3.75-times in the improved protocol for all the tested concentrations of the target DNA strand of 1000, 100, 10, 3.3, and 2 nM, respectively. The developed biosensor system may allow the detection of the pathogenic fungus in postharvest produce and determine its pathogenicity state.

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Section: Resultsmentioning

confidence: 99%

The Incorporation of Amplified Metal-Enhanced Fluorescence in a CMOS-Based Biosensor Increased the Detection Sensitivity of a DNA Marker of the Pathogenic Fungus Colletotrichum gloeosporioides

2020

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“…The antimicrobial character of AgNPs and silicone hydrogel-based composite films against S. aureus , P. aeruginosa , and E. coli was also investigated by Mourad et al The composite films proved their potential in contact lens applications [ 158 ]. Furthermore, Cruz-Pacheco et al coated polyetheretherketone with one or two layers of AgNPs to inhibit E. coli , S. marcescens , and B. licheniformis growth for potential biomedical applications [ 159 ]. Additionally, López-Saucedo et al developed polytetrafluorethylene films grafted with methyl methacrylate and subsequently with N-vinylimidazole using gamma-rays that were used for the immobilization of AgNPs.…”

Section: Inorganic Nanoparticle-based Composite Films For Antimicrobial Applicationsmentioning

confidence: 99%

Inorganic Nanoparticles and Composite Films for Antimicrobial Therapies

Spirescu

Chircov

Grumezescu

et al. 2021

IJMS

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The development of drug-resistant microorganisms has become a critical issue for modern medicine and drug discovery and development with severe socio-economic and ecological implications. Since standard and conventional treatment options are generally inefficient, leading to infection persistence and spreading, novel strategies are fundamentally necessary in order to avoid serious global health problems. In this regard, both metal and metal oxide nanoparticles (NPs) demonstrated increased effectiveness as nanobiocides due to intrinsic antimicrobial properties and as nanocarriers for antimicrobial drugs. Among them, gold, silver, copper, zinc oxide, titanium oxide, magnesium oxide, and iron oxide NPs are the most preferred, owing to their proven antimicrobial mechanisms and bio/cytocompatibility. Furthermore, inorganic NPs can be incorporated or attached to organic/inorganic films, thus broadening their application within implant or catheter coatings and wound dressings. In this context, this paper aims to provide an up-to-date overview of the most recent studies investigating inorganic NPs and their integration into composite films designed for antimicrobial therapies.

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“…On the urban scale, they might reduce the heat island effect and sewage system load, improve runoff water and air quality, and reconstruct natural landscapes including wildlife. In summary, this e-book on coatings compiles the following Special Issues demonstrating the bright future of advanced coatings for buildings: "Advanced Protective Coatings for Buildings" [2,3], "Nanocoatings with Air-Purifying Properties" [4][5][6], "Antifouling Coatings" [7][8][9][10], "Ultra-Low Biofouling Materials and Coatings" [11] and "Biological Coatings for Buildings" [12][13][14][15][16]. Going into detail, the Special Issues include the following topics:…”

Section: Multiperformance Durable and Sustainable Coatingsmentioning

confidence: 99%