Coat Protein-Mediated Resistance Against Virus Infection

Beachy, Roger N.; Loesch-Fries, Sue; Tumer, Nilgun E.

doi:10.1146/annurev.py.28.090190.002315

Cited by 393 publications

(128 citation statements)

References 42 publications

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“…Most examples of PDR involve transgenic plants engineered to express a viral coat protein (CP) or a segment of a replicase gene (for reviews, see Beachy et al, 1990; Wilson, 1993). As a general rule, transgenic plants accumulating one of these viral proteins are often resistant to that particular virus and closely related viruses.…”

Section: Introductionmentioning

confidence: 99%

Induction of a Highly Specific Antiviral State in Transgenic Plants: Implications for Regulation of Gene Expression and Virus Resistance.

et al. 1993

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Transgenic tobacco plants expressing either a full-length form of the tobacco etch virus (TEV) coat protein or a form truncated at the N terminus of the TEV coat protein were initially susceptible to TEV infection, and typlcal systemic symptoms developed. However, 3 to 5 weeks after a TEV infection was established, transgenlc plants "recovered" from the TEV infection, and new stem and leaf tissue emerged symptom and virus free. A TEV-resistant state was induced ln the recovered tissue. The resistance was virus speclflc. Recovered plant tissue could not be lnfected wlth TEV, but was susceptlble to the closely related virus, potato virus Y. The resistance phenotype was functional at the slngle-cell leve1 because protoplasts from recovered transgenic tissue did not support TEV repllcation. Surprlslngly, steady state transgene mRNA levels in recovered tissue were 12-to 22-fold less than transgene mRNA levels in uninoculated transgenic tissue of the same developmental stage. However, nuclear run-off studles suggested that transgene transcription rates in recovered and uninoculated plants were similar. We propose that the resistant state and reduced steady state levels of transgene transcript accumulation are mediated at the cellular leve1 by a cytoplasmic actlvity that targets speclfic RNA sequences for inactlvation.

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Section: Introductionmentioning

confidence: 99%

Induction of a Highly Specific Antiviral State in Transgenic Plants: Implications for Regulation of Gene Expression and Virus Resistance.

et al. 1993

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“…Okuno et al (1993b) have also reported higher virus accumulation in the inoculated leaves of transgenic plants expressing the CP gene than in the systemic leaves. As reviewed by Beachy et al (1990), the CPMR is manifested by a reduced rate of systemic spread of infection whereby development of systemic disease symptoms in CP (+) plants is either completely inhibited or much reduced than the non-transgenic controls so that even if the inoculation resulted in infection on the inoculated leaves, the likelihood of the infection becoming systemic is reduced. Similarly, Nelson et al (1987) have also reported that the delay in systemic symptom development in transgenic tobacco expressing the CP gene of TMV was correlated with reduction in lesion numbers and virus accumulation in the transgenic plants.…”

Section: Discussionmentioning

confidence: 99%

“…First engineered resistance against CMV utilizing the coat protein (CP) gene was demonstrated by Cuozzo et al (1988). Since then, many examples of CP-mediated resistance (CPMR) in varying degrees have been described, with different constructs in different hosts (Beachy et al 1990;Srivastava & Raj 2008). Different mechanisms also appear to be responsible for protection, depending on the virus group or the viral transgene studied (Loesch-Fries et al 1987;Lomonossoff 1995;Gururani et al, 2012).…”

Section: Introduction Cucumber Mosaic Virus (Cmv) Is a Member Of The mentioning

confidence: 99%

Expression of coat protein gene ofCucumber mosaic virus(CMV-subgroup IA)Gladiolusisolate inNicotiana tabacum

Dubey

Chandrasekhar

Srivastava³

et al. 2015

Journal of Plant Interactions

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The coat protein (CP)-mediated resistance against Cucumber mosaic virus (CMV) subgroup IA was developed in transgenic lines of Nicotiana tabacum cv. Petit Havana using Agrobacterium tumefaciens-mediated transformation. Ten independently transformed lines have developed, four of which were tested for resistance against CMV using virus challenge inoculations. The transgenic lines exhibiting complete resistance remained healthy and symptomless in their life span and showed reduced or no virus accumulation in their systemic leaves after virus challenge inoculation. These transgenic lines also showed resistance against CMV strains which are not closely related to CMV-Gladiolus strains. This is the first report of CP-mediated transgenic resistance against a CMV subgroup IA member isolated from India showing resistance to all CMV strains occurring in the same vicinity.

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“…This type of resistance, generally referred to as coat protein-mediated resistance, has been described for many virus/host systems, but is restricted to the virus closely related to the introduced gene (Beachy et al 1990). …”

Section: Introductionmentioning

confidence: 99%

Development of Breeding Materials of Transgenic Tomato Plants with a Truncated Replicase Gene of Cucumber Mosaic Virus for Resistance to the Virus.

et al. 2002

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Tomato plants were transformed with a truncated replicase gene encoded by RNA 2 of cucumber mosaic virus (CMV) strain GT, subgroup II. The truncated replicase gene does not retain a C-terminal region of the gene that contains the GDD amino acid motif and the NTP binding motif. These motifs are considered to correspond to active and/or recognition domains of RNAdependent RNA polymerase. Upon transformation via Agrobacterium tumefaciens, 137 individual transgenic lines were obtained. Each transgenic line was evaluated for resistance to CMV. About 10 % of the transgenic lines were highly resistant and the remaining 90 % showed a moderate resistance or were susceptible. The 15 lines were selected as resistant lines. Chenopodium amaranticolor was used to analyze the multiplication of CMV in the symptom-less plants. Among the selected lines, three lines did not appear to show any multiplication of CMV in both inoculated and non-inoculated leaves. Based on transgene amplification by PCR and the kanamycin resistance assay, the T 1 progeny of the selected lines harbored the transgene. Several resistant lines of the T 1 generation were resistant to viral inoculation. These resistant lines could become suitable breeding materials for resistance to CMV.

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Coat Protein-Mediated Resistance Against Virus Infection

Cited by 393 publications

References 42 publications

Induction of a Highly Specific Antiviral State in Transgenic Plants: Implications for Regulation of Gene Expression and Virus Resistance.

Induction of a Highly Specific Antiviral State in Transgenic Plants: Implications for Regulation of Gene Expression and Virus Resistance.

Expression of coat protein gene ofCucumber mosaic virus(CMV-subgroup IA)Gladiolusisolate inNicotiana tabacum

Development of Breeding Materials of Transgenic Tomato Plants with a Truncated Replicase Gene of Cucumber Mosaic Virus for Resistance to the Virus.

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