Coat Protein Gene Replacement Results in Whitefly Transmission of an Insect Nontransmissible Geminivirus Isolate

Höfer, Petra; Bedford, Ian; Markham, P. G.; Jeske, Holger; Frischmuth, Thomas

doi:10.1006/viro.1997.8751

Cited by 108 publications

(72 citation statements)

References 35 publications

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“…4B). These results confirmed the essential role of viral CP in vector-mediated virus transmission (42,44).…”

Section: Retention Of Virions In Psgs Is a Prerequisite For Virus Trasupporting

confidence: 79%

“…Replacement of CP from the nontransmissible Abutilon mosaic virus (AbMV) with CP of the transmissible Sida golden mosaic virus (SiGMV) resulted in a transmissible AbMV chimera (44). The region of CP between amino acids 129 and 134 was shown to be involved in whiteflyspecific transmission of TYLCV and correlated with virion formation (45).…”

Section: Discussionmentioning

confidence: 99%

“…The CP of begomoviruses is the only virus-encoded protein that has been shown to determine vector competence (42)(43)(44). To investigate whether viral CP is implicated in virus transmission, we exchanged a partial CP sequence of TYLCV which has been shown to influence virus transmissibility (44,45) with that of TYLCCNV (Fig. 3) and examined the transmissibility of the two mutant viruses by MEAM1 and MED whiteflies.…”

Section: Retention Of Virions In Psgs Is a Prerequisite For Virus Tramentioning

confidence: 99%

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Specific Cells in the Primary Salivary Glands of the Whitefly Bemisia tabaci Control Retention and Transmission of Begomoviruses

Wei

Zhao

Zhang

et al. 2014

J Virol

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The majority of plant viruses are vectored by arthropods via persistent-circulative or noncirculative transmission. Previous studies have shown that specific binding sites for noncirculative viruses reside within the stylet or foregut of insect vectors, whereas the transmission mechanisms of circulative viruses remain ambiguous. Here we report the critical roles of whitefly primary salivary glands (PSGs) in the circulative transmission of two begomoviruses. The Middle East Asia Minor 1 (MEAM1) species of the whitefly Bemisia tabaci complex efficiently transmits both Tomato yellow leaf curl China virus (TYLCCNV) and Tomato yellow leaf curl virus (TYLCV), whereas the Mediterranean (MED) species transmits TYLCV but not TYLCCNV. PCR and fluorescence in situ hybridization experiments showed that TYLCCNV efficiently penetrates the PSGs of MEAM1 but not MED whiteflies. When a fragment of the coat protein of TYLCCNV was exchanged with that of TYLCV, mutated TYLCCNV accumulated in the PSGs of MED whiteflies, while mutant TYLCV was nearly undetectable. Confocal microscopy revealed that virion transport in PSGs follows specific paths to reach secretory cells in the central region, and the accumulation of virions in the secretory region of PSGs was correlated with successful virus transmission. Our findings demonstrate that whitefly PSGs, in particular the cells around the secretory region, control the specificity of begomovirus transmission. IMPORTANCEOver 75% of plant viruses are transmitted by insects. However, the mechanisms of virus transmission by insect vectors remain largely unknown. Begomoviruses and whiteflies are a complex of viruses and vectors which threaten many crops worldwide. We investigated the transmission of two begomoviruses by two whitefly species. We show that specific cells of the whitefly primary salivary glands control viral transmission specificity and that virion transport in the glands follows specific paths to reach secretory cells in the central region and then to reach the salivary duct. Our results indicate that the secretory cells in the central region of primary salivary glands determine the recognition and transmission of begomoviruses. These findings set a foundation for future research not only on circulative plant virus transmission but also on other human and animal viruses transmitted by arthropod vectors.

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“…4B). These results confirmed the essential role of viral CP in vector-mediated virus transmission (42,44).…”

Section: Retention Of Virions In Psgs Is a Prerequisite For Virus Trasupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Retention Of Virions In Psgs Is a Prerequisite For Virus Tramentioning

confidence: 99%

See 1 more Smart Citation

Specific Cells in the Primary Salivary Glands of the Whitefly Bemisia tabaci Control Retention and Transmission of Begomoviruses

Wei

Zhao

Zhang

et al. 2014

J Virol

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“…Coat protein gene of CpCDV was replaced by CLCuBuV by removal of the coat protein coding region of CpCDV using polymerase chain reaction (PCR) amplification (Hofer et al, 1997). The machine was programmed for a preheat treatment of 94˚C for 5 min pursued by 30 cycles of 94˚C for 1 min, 55˚C for 1 min and 72˚C for 2 min.…”

Section: Construction Of Recombinant Constructs Of Cpcdv and Clcubuv-mentioning

confidence: 99%

“…However, by replacing the coat protein gene of African cassava mosaic virus (ACMV; whitefly transmissible begomovirus) with BCTV-CP using appropriate restriction enzymes switched its transmission from leafhopper to whitefly (Briddon et al, 1990). Hofer et al (1997) conducted the same experiment on whitefly transmissible and nontransmissible begomoviruses i.e., Sida golden mosaic virus' (SiGMV-Co) and Abutilon mosaic virus' (AbMV), respectively, by applying polymerase chain reaction with specific primers (instead of using restriction enzymes). The resultant AbMV changed its transmission behavior from non-transmissibility to successful transmission.…”

Section: Introductionmentioning

confidence: 99%

Construction of an Infectious Chimeric Geminivirus by Molecular Cloning Based on Coinfection and Recombination

Khalid¹,

Zia-ur-Rehman²,

Ali³

et al. 2017

IJAB

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In the evolution of geminiviruses, genetic recombination plays a key role in ecological, biochemical and evolutionary processes. They evolve as results of prehistoric inter-genus recombination whereas intra-genus recombination of geminiviruses leads to the emergence of agriculturally important plant pathogens. Geminiviruses are transmitted by leafhoppers, treehoppers and whiteflies. An isolate of Chickpea chlorotic dwarf virus (CpCDV), a monopartite dicot infecting mastrevirus is transmitted by leafhopper. In contrast, Cotton leaf curl burewala virus (CLCuBuV), a monopartite begomovirus, was acquired and transmissible by whitefly to various host plants. In this study, we remove the coat protein (CP) gene of CpCDV by PCR and replaced with that of PCR amplified CLCuBuV-CP. The resultant infectious clone of chimeric CpCDV is produced later named mastrebegomo chimera (pGII0000MBC). By using Agroinfiltration technique (Agrobacterium-mediated inoculation) the chimeric clone along with wild type Clone CpCDV and CLCuBuV are injected on tomato (Lycopersicon esculentum Mill.) and tobacco (Nicotiana benthamiana Domin.) seedlings and get symptoms after 21dpi (days post inoculation). Later, the symptoms appeared on plants were compared with healthy plants. This study elaborates our awareness about the genetic recombination and coexistence of mastreviruses with begomovirus globally including Pakistan and will provide the basic information regarding their management.

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Transmission specificities of plant viruses with the newly identified species of the Bemisia tabaci species complex

Polston

Barro

Boykin

2014

Pest Management Science

187

113

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Bemisia tabaci has had a colorful nomenclatural past and is now recognized as a species complex. This new species framework has added many new areas of research including adding new insight into the virus transmission specificity of the species in the B. tabaci species complex. There is a wide disparity in what is known about the transmission of plant viruses by different members of the B. tabaci species complex. In this paper, we have synthesized the transmission specificities of the plant viruses transmitted by species belonging to the complex. There are five genera of plant viruses with members that are transmitted by species of the B. tabaci species complex. The transmission of viruses belonging to two of these, Begomovirus and Crinivirus, are well studied and much is known in regards to the relationship between species and transmission and etiology. This is in contrast to viruses of the genera, Torradovirus and Carlavirus, for which very little is known inregards to their transmission. This is the first attempt to integrate viral data within the new B. tabaci species complex framework. It is clear that matching historical transmission data with the current species framework is difficult due to the lack of awareness of the underlying genetic diversity within B. tabaci. We encourage all researchers to determine which species of B. tabaci they are using to facilitate association of phenotypic traits with particular members of the complex.

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Coat Protein Gene Replacement Results in Whitefly Transmission of an Insect Nontransmissible Geminivirus Isolate

Cited by 108 publications

References 35 publications

Specific Cells in the Primary Salivary Glands of the Whitefly Bemisia tabaci Control Retention and Transmission of Begomoviruses

Specific Cells in the Primary Salivary Glands of the Whitefly Bemisia tabaci Control Retention and Transmission of Begomoviruses

Construction of an Infectious Chimeric Geminivirus by Molecular Cloning Based on Coinfection and Recombination

Transmission specificities of plant viruses with the newly identified species of the Bemisia tabaci species complex

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