Coassembly Behavior and Rheological Properties of a β-Hairpin Peptide with Dicarboxylates

Ge, Yanqing; Wang, Chen; Zhang, Weiqiang; Lai, Shike; Wang, Dong; Wang, Jiqian

doi:10.1021/acs.langmuir.1c01376

Cited by 4 publications

(2 citation statements)

References 33 publications

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“…In addition, PI is a red-fluorescent membrane-impermeable drug molecule, and thus is frequently used as a marker for live/dead cell detection. 47–50 It was found that Pep1–PO 4 3− nanoblocks carrying PI molecules can be taken up by HeLa cells, and red fluorescence can be detected in the HeLa cells (Fig. 6), indicating that PI can be released from Pep1–PO 4 3− nanoblocks into the cytoplasm and nucleus rather than entering the cells due to toxicity.…”

Section: Resultsmentioning

confidence: 98%

Self-assembled peptide-based nanoblocks for drug delivery

Xie,

Meng,

Zhang

et al. 2023

New J. Chem.

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Section: Resultsmentioning

confidence: 98%

Self-assembled peptide-based nanoblocks for drug delivery

Xie,

Meng,

Zhang

et al. 2023

New J. Chem.

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“…Next, calcein-AM is cleaved by intracellular esterases to yield highly fluorescent and polar calcein (208)(209)(210). When cells are incubated with calcein and co 2+ , both enter the cytoplasm; however, calcein is further captured by mitochondria (211,212). calcein that accumulates in the mitochondria exhibits fluorescent staining, whereas calcein remaining in the cytoplasm or released from the mitochondria into the cytoplasm is rapidly quenched by co 2+ (213)(214)(215)(216)(217)(218)(219).…”

Section: Detection Of Mitochondrial Permeability Transition Poresmentioning

confidence: 99%

Common methods in mitochondrial research (Review)

Yin

Shen

2022

Int J Mol Med

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Mitochondrial abnormalities are primarily seen in morphology, structure and function. They can cause damage to organs, including the heart, brain and muscle, by various mechanisms, such as oxidative stress, abnormal energy metabolism, or genetic mutations. Identifying and detecting pathophysiological alterations in mitochondria is the principal means of studying mitochondrial abnormalities. The present study reviewed methods in mitochondrial research and focused on three aspects: Mitochondrial extraction and purification, morphology and structure and function. In addition to classical methods, such as electron microscopy and mitochondrial membrane potential monitoring, newly developed methods, such as mitochondrial ultrastructural determination, mtdNA mutation assays, metabolomics and analyses of regulatory mechanisms, have also been utilized in recent years. These approaches enable the accurate detection of mitochondrial abnormalities and provide guidance for the diagnosis and treatment of related diseases. Contents1. Introduction 2. Extraction and purification of mitochondria 3. determination of mitochondrial morphology and structure 4. determination of mitochondrial function 5.

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Biomineralization-Inspired Anti-Caries Strategy Based on Multifunctional Nanogels as Mineral Feedstock Carriers

Yuan,

Zhang,

Liao

et al. 2023

IJN

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Background: Dentin caries remains a significant public concern, with no clinically viable material that effectively combines remineralization and antimicrobial properties. To address this issue, this study focused on the development of a bio-inspired multifunctional nanogel with both antibacterial and biomineralization properties. Methods: First, p(NIPAm-co-DMC) (PNPDC) copolymers were synthesized from N-isopropylacrylamide (NIPAm) and 2-methacryloyloxyethyl-trimethyl ammonium chloride (DMC). Subsequently, PNPDC was combined with γ-polyglutamic acid (γ-PGA) through physical cross-linking to form nanogels. These nanogels served as templates for the mineralization of calcium phosphate (Cap), resulting in Cap-loaded PNPDC/PGA nanogels. The nanogels were characterized using various techniques, including TEM, particle tracking analysis, XRD, and FTIR. The release properties of ions were also assessed. In addition, the antibacterial properties of the Cap-loaded PNPDC/PGA nanogels were evaluated using the broth microdilution method and a biofilm formation assay. The remineralization effects were examined on both demineralized dentin and type I collagen in vitro. Results: PNPDC/PGA nanogels were successfully synthesized and loaded with Cap. The diameter of the Cap-loaded PNPDC/PGA nanogels was measured as 196.5 nm at 25°C and 162.3 nm at 37°C. These Cap-loaded nanogels released Ca 2+ and PO 4 3ions quickly, effectively blocking dental tubules with a depth of 10 μm and promoting the remineralization of demineralized dentin within 7 days. Additionally, they facilitated the heavy intrafibrillar mineralization of type I collagen within 3 days. Moreover, the Cap-loaded nanogels exhibited MIC 50 and MIC 90 values of 12.5 and 50 mg/mL against Streptococcus mutans, respectively, with an MBC value of 100 mg/mL. At a concentration of 50 mg/mL, the Cap-loaded nanogels also demonstrated potent inhibitory effects on biofilm formation by Streptococcus mutans while maintaining good biocompatibility. Conclusion:Cap-loaded PNPDC/PGA nanogels are a multifunctional biomimetic system with antibacterial and dentin remineralization effects. This strategy of using antibacterial nanogels as mineral feedstock carriers offered fresh insight into the clinical management of caries.

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Coassembly Behavior and Rheological Properties of a β-Hairpin Peptide with Dicarboxylates

Cited by 4 publications

References 33 publications

Self-assembled peptide-based nanoblocks for drug delivery

Self-assembled peptide-based nanoblocks for drug delivery

Common methods in mitochondrial research (Review)

Biomineralization-Inspired Anti-Caries Strategy Based on Multifunctional Nanogels as Mineral Feedstock Carriers

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