Coassembled nanostructured bioscaffold reduces the expression of proinflammatory cytokines to induce apoptosis in epithelial cancer cells

Li, Rui; Pavuluri, Sivapriya; Bruggeman, Kiara F.; Long, Benjamin M.; Parnell, Andrew J.; Martel, Anne; Parnell, Steven R.; Pfeffer, Frederick M.; Dennison, Andrew J. C.; Nicholas, Kevin R.; Barrow, Colin J.; Nisbet, David R.; Williams, Richard J.

doi:10.1016/j.nano.2016.01.009

Cited by 40 publications

(50 citation statements)

References 58 publications

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“…Consequently, adsorption prevents protein unfolding until the time at which they are released via diffusion. Previously, we have characterised this effect with other macromolecules such as fucoidan, observing their close association with the bundles of nanofibres, and our ability to stabilise soluble molecules within our minimalistic SAP hydrogels (Horgan et al, 2016;Li et al, 2017;Li et al, 2016); however, this is the first reported demonstration of this process with molecules where the maintenance of structure is vital to their function.…”

Section: Fmoc-dikvav Impedes Gdnf Unfolding Resulting In Sustained mentioning

confidence: 84%

Using minimalist self‐assembling peptides as hierarchical scaffolds to stabilise growth factors and promote stem cell integration in the injured brain

Rodriguez

Bruggeman

Wang

et al. 2017

J Tissue Eng Regen Med

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Neurotrophic growth factors are effective in slowing progressive degeneration and/or promoting neural repair through the support of residual host and/or transplanted neurons. However, limitations including short half-life and enzyme susceptibility of growth factors highlight the need for alternative strategies to prolong localised delivery at a site of injury. Here, we establish the utility of minimalist N-fluorenylmethyloxycarbonyl (Fmoc) self-assembling peptides (SAPs) as growth factor delivery vehicle, targeted at supporting neural transplants in an animal model of Parkinson's disease. The neural tissue-specific SAP, Fmoc-DIKVAV, demonstrated sustained release of glial cell line derived neurotrophic factor, up to 172 hr after gel loading. This represents a significant advance in drug delivery, because its lifetime in phosphate buffered saline was less than 1 hr. In vivo transplantation of neural progenitor cells, together with our growth factor-loaded material, into the injured brain improved graft survival compared with cell transplants alone. We show for the first time the use of minimalist Fmoc-SAP in an in vivo disease model for sustaining the delivery of neurotrophic growth factors, facilitating their spatial and temporal delivery in vivo, whilst also providing an enhanced niche environment for transplanted cells.

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Section: Fmoc-dikvav Impedes Gdnf Unfolding Resulting In Sustained mentioning

confidence: 84%

Using minimalist self‐assembling peptides as hierarchical scaffolds to stabilise growth factors and promote stem cell integration in the injured brain

Rodriguez

Bruggeman

Wang

et al. 2017

J Tissue Eng Regen Med

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“…Peptide concentration of 1 wt% was used to co‐assemble with either fucoidan or versican and gelation took place through pH switch . The presence of macromolecules affected the self‐assembly of fibers into bundles, as shown in the Fmoc‐FRGDF + fucoidan . However, the fibrils formed with Fmoc‐FRGDF + versican showed rather different morphologies than the former scaffolds .…”

Section: Self‐assembling Hydrogels Of Peptides With Biopolymersmentioning

confidence: 99%

Supramolecular Peptide/Polymer Hybrid Hydrogels for Biomedical Applications

Radvar

Azevedo

2018

Macromolecular Bioscience

123

107

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Peptides and polymers are the "elite" building blocks in hydrogel fabrication where the typical approach consists of coupling specific peptide sequences (cell adhesive and/or enzymatically cleavable) to polymer chains aiming to obtain controlled cell responses (adhesion, migration, differentiation). However, the use of polymers and peptides as structural components for fabricating supramolecular hydrogels is less well established. Here, the literature on the design of peptide/polymer systems for self-assembly into hybrid hydrogels, as either peptide-polymer conjugates or combining both components individually, is reviewed. The properties (stiffness, mesh structure, responsiveness, and biocompatibility) of the hydrogels are then discussed from the viewpoint of their potential biomedical applications.

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“…[21][22][23] An alternative strategy is to incorporate the functional payload, in this case the RGD motif, within the peptide backbone. Although this is a similar strategy to that of Fmoc-FRGDF and other examples where the functional motif is introduced within the sequence, 24,25 the introduction of RGD and other epitopes within the Fmoc self-assembling peptide sequence would not be expected to affect self-assembly as the Fmoc group is responsible for self-assembly. There are also other notable examples where a functional motif is introduced between two b-sheet forming a-peptides.…”

mentioning

confidence: 99%

β3-tripeptides act as sticky ends to self-assemble into a bioscaffold

et al. 2018

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Peptides comprised entirely of β 3 -amino acids, commonly referred to as β-foldamers, have been shown to self-assemble into a range of materials. Previously, β-foldamers have been functionalised via various side chain chemistries to introduce function to these materials without perturbation of the self-assembly motif. Here, we show that insertion of both rigid and flexible molecules into the backbone structure of the β-foldamer did not disturb the self-assembly, provided that the molecule is positioned between two β 3 -tripeptides. These hybrid β 3 -peptide flanked molecules self-assembled into a range of structures. α-Arginlyglycylaspartic acid (RGD), a commonly used cell attachment motif derived from fibronectin in the extracellular matrix, was incorporated into the peptide sequence in order to form a biomimetic scaffold that would support neuronal cell growth. The RGD-containing sequence formed the desired mesh-like scaffold but did not encourage neuronal growth, possibly due to over-stimulation with RGD. Mixing the RGD peptide with a β-foldamer without the RGD sequence produced a well-defined scaffold that successfully encouraged the growth of neurons and enabled neuronal electrical functionality. These results indicate that β 3 -tripeptides can form distinct self-assembly units separated by a linker and can form fibrous assemblies. The linkers within the peptide sequence can be composed of a bioactive α-peptide and tuned to provide a biocompatible scaffold.

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Coassembled nanostructured bioscaffold reduces the expression of proinflammatory cytokines to induce apoptosis in epithelial cancer cells

Cited by 40 publications

References 58 publications

Using minimalist self‐assembling peptides as hierarchical scaffolds to stabilise growth factors and promote stem cell integration in the injured brain

Using minimalist self‐assembling peptides as hierarchical scaffolds to stabilise growth factors and promote stem cell integration in the injured brain

Supramolecular Peptide/Polymer Hybrid Hydrogels for Biomedical Applications

β3-tripeptides act as sticky ends to self-assemble into a bioscaffold

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