Coal-vitamin medium for improved scheme of isolating biosurfactant-producing actinomycetes of rare species from soil samples

Ayoib, A.; Gopinath, Subash C. B.; Yahya, Ahmad Shukri; Zakaria, Latiffah

doi:10.1007/s13399-022-03691-8

Cited by 4 publications

(3 citation statements)

References 98 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In ODM, the surfactant-containing supernatant displaces oil on the water's surface, with the clear zone's size indicating the surfactant concentration. Since lowmolecular-mass biosurfactants like glycolipids and lipopeptides are effective in reducing surface and interfacial tension [24,40], surfactant-containing supernatants will spread or collapse on hydrophobic surfaces, such as crude oil. Therefore, DCM and ODM are valuable screening techniques for detecting low-molecular mass biosurfactants.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Screening biosurfactant‐producing actinomycetes: Identification of Streptomyces sp. RP1 as a potent species for bioremediation

Ayoib,

Gopinath,

Zambry

et al. 2024

J Basic Microbiol

Self Cite

View full text Add to dashboard Cite

This study aimed to isolate biosurfactant‐producing and hydrocarbon‐degrading actinomycetes from different soils using glycerol–asparagine and starch–casein media with an antifungal agent. The glycerol–asparagine agar exhibited the highest number of actinomycetes, with a white, low‐opacity medium supporting pigment production and high growth. Biosurfactant analyses, such as drop collapse, oil displacement, emulsification, tributyrin agar test, and surface tension measurement, were conducted. Out of 25 positive isolates, seven could utilize both olive oil and black oil for biosurfactant production, and only isolate RP1 could produce biosurfactant when grown in constrained conditions with black oil as the sole carbon source and inducer, demonstrating in situ bioremediation potential. Isolate RP1 from oil‐spilled garden soil is Gram‐staining‐positive with a distinct earthy odor, melanin formation, and white filamentous colonies. It has a molecular size of ~621 bp and 100% sequence similarity to many Streptomyces spp. Morphological, biochemical, and 16 S rRNA analysis confirmed it as Streptomyces sp. RP1, showing positive results in all screenings, including high emulsification activity against kerosene (27.2%) and engine oil (95.8%), oil displacement efficiency against crude oil (7.45 cm), and a significant reduction in surface tension (56.7 dynes/cm). Streptomyces sp. RP1 can utilize citrate as a carbon source, tolerate sodium chloride, resist lysozyme, degrade petroleum hydrocarbons, and produce biosurfactant at 37°C in a 15 mL medium culture, indicating great potential for bioremediation and various downstream industrial applications with optimization.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Experiments were conducted using modified compositions of SC and GA media in favor of actinomycetes growth [24]. The SC (g/L) liquid medium was composed of potato starch, 10.0; casein, 0.3; KNO 3 , 2.0; NaCl, 2.0; K 2 HPO 4 , 2.0; FeSO 4 ‧7H 2 O, 0.01; MgSO 4 ‧7H 2 O, 0.05.…”

Section: Growth Culture Media and Storage Of Bacterial Isolatesmentioning

confidence: 99%

Screening biosurfactant‐producing actinomycetes: Identification of Streptomyces sp. RP1 as a potent species for bioremediation

Ayoib,

Gopinath,

Zambry

et al. 2024

J Basic Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…58 The collected soil samples 10cm below the surface were treated prior to the isolation by exposing to air and sieved, the obtained soil sample was subjected at 30°C in an hot air oven for a week, 40°C exposure of the samples was done in order to reduce the proportion of bacteria and other actinomycetes while preserving the spores of actinomycetes. 59 A different pretreatment involved mixing1 g of air-dried soil with 10 mL of sterile 5 mM phosphate buffer pH 7.2, (i) peptone (6%), and (ii) yeast extract (6%), followed by a heat shock at 50°C for 10 minutes at 200 rpm in an incubator shaker. Inhibiting the growth of Gram-negative bacteria and other unrelated soil bacteria while actinomycetes spores germinate.…”

Section: Treatment Of Soil Samplementioning

confidence: 99%

Actinomycetes: Isolation, Cultivation and its Active Biomolecules

Meenakshi,

Hiremath,

Meenakshi

et al. 2024

J. Pure Appl. Microbiol.

View full text Add to dashboard Cite

Actinomycetes are the potential producers of secondary metabolites of vivid applications; they are isolated from almost all the sources both terrestrial and aquatic habitats. Actinomycetes are a group of Gram-positive bacteria known for their filamentous structure and ability to produce a diverse array of bioactive compounds. These bioactive compounds include antibiotics, antifungals, antivirals, anticancer agents, immunosuppressants, and enzymes. Actinomycetes have been a major source of these bioactive compounds and have played a significant role in the development of many therapeutic drugs. Actinomycetes, which are isolated from practically all sources in both terrestrial and aquatic ecosystems, have the potential to create secondary metabolites with diverse uses. A class of Gram-positive bacteria called actinomycetes is distinguished by its filamentous structure and capacity to manufacture a wide range of bioactive substances. Antibiotics, antifungals, antivirals, cancer preventatives, immunosuppressants, and enzymes are a few examples of these bioactive substances. These bioactive substances have primarily come from actinomycetes, which have also contributed significantly to the creation of several medicinal medications. However, actinomycetes isolation and cultivation can be challenging due to their slow growth rate and complex nutritional requirements. In order to isolate and cultivate actinomycetes, several pre-treatment methods and media can be employed.

show abstract

Biotechnological potentials of surfactants in coal utilization: a review

Akimbekov,

Digel,

Zhubanova

et al. 2024

Environ Sci Pollut Res

View full text Add to dashboard Cite

The quest for scientifically advanced and sustainable solutions is driven by growing environmental and economic issues associated with coal mining, processing, and utilization. Consequently, within the coal industry, there is a growing recognition of the potential of microbial applications in fostering innovative technologies. Microbial-based coal solubilization, coal beneficiation, and coal dust suppression are green alternatives to traditional thermochemical and leaching technologies and better meet the need for ecologically sound and economically viable choices. Surfactant-mediated approaches have emerged as powerful tools for modeling, simulation, and optimization of coal-microbial systems and continue to gain prominence in clean coal fuel production, particularly in microbiological co-processing, conversion, and beneficiation. Surfactants (surface-active agents) are amphiphilic compounds that can reduce surface tension and enhance the solubility of hydrophobic molecules. A wide range of surfactant properties can be achieved by either directly influencing microbial growth factors, stimulants, and substrates or indirectly serving as frothers, collectors, and modifiers in the processing and utilization of coal. This review highlights the significant biotechnological potential of surfactants by providing a thorough overview of their involvement in coal biodegradation, bioprocessing, and biobeneficiation, acknowledging their importance as crucial steps in coal consumption.

show abstract

Coal-vitamin medium for improved scheme of isolating biosurfactant-producing actinomycetes of rare species from soil samples

Cited by 4 publications

References 98 publications

Screening biosurfactant‐producing actinomycetes: Identification of Streptomyces sp. RP1 as a potent species for bioremediation

Screening biosurfactant‐producing actinomycetes: Identification of Streptomyces sp. RP1 as a potent species for bioremediation

Actinomycetes: Isolation, Cultivation and its Active Biomolecules

Biotechnological potentials of surfactants in coal utilization: a review

Contact Info

Product

Resources

About