Coagulation factors and inhibitors in thawed plasma stored at 1–6°C for 5days in China

Wang, Zongkui; Du, Xi; Li, Changqing; Ma, Li; Sun, Pengfei; Cao, Haijun; Lin, Fen; Ye, Shengliang; Xiao, Xiaopu

doi:10.1016/j.transci.2014.01.002

Cited by 10 publications

(21 citation statements)

References 34 publications

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“…In our previous studies, we found that ABO blood group, age, and gender influenced the circulating concentrations of some plasma proteins, such as fibrinogen (FG), coagulation factor (F) VIII, von Willebrand factor, and ADAMTS13 (a disintegrin and metalloproteinase with thrombospondin motifs 13) . Therefore, in order to reduce the sample selection bias, we used an ABO type‐, gender‐, and age‐matched model to compare the plasma proteome profiles of Bama longevity hotspot (case study) with other non‐longevity area (control).…”

Section: Discussionmentioning

confidence: 99%

TMT‐Based Quantitative Proteomic Analysis Reveals Proteomic Changes Involved in Longevity

Wang

Zhang

Liu

et al. 2018

Proteomics Clinical Apps

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Purpose: Individual lifespans vary widely, and longevity is the main concern from ancient to modern times. This study is aimed to identify plasma proteins associated with longevity by proteomics technique. Experimental design: Tandem mass tags (TMT)-based proteomics analysis is performed for the plasma of Bama longevity group and a control group to analyze the differentially expressed proteins (DEPs). A validation set is used to verify the results of TMT-based proteomics.Results: Between Bama natives and the control individuals, the authors identify 175 DEPs, which are mainly involved in complement and coagulation cascades, metabolism of glyco and lipid, and regulation of actin cytoskeleton. Consistent with the proteomic analysis, plasma levels of MMP2, CCL5, and PF4 are significantly lower in Bama participants than in controls, whereas IGFBP2 and C9 increase in Bama individuals, in the validation set. By ROC analysis, combinations of these five proteins result in a high AUC value (0.991, 95% CI, 0.929-1.000, p < 0.0001) to distinguish longevous participants from controls. Conclusions and clinical relevance: The results highlight the roles of complement and coagulation cascades, metabolism of glyco and lipid, and inflammatory and immune response may play important roles in longevity.And the DEPs may serve as clinically useful biomarkers for healthy aging and predicting longevity.

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Section: Discussionmentioning

confidence: 99%

TMT‐Based Quantitative Proteomic Analysis Reveals Proteomic Changes Involved in Longevity

Wang

Zhang

Liu

et al. 2018

Proteomics Clinical Apps

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“…Of note, an extension of the storage time from 3 to 7 days will not influence the quality of clotting factors. [ 22 23 ] The waste of FFPs was 4 times higher in the patients for whom the MTP was activated but who turned out not to be massive bleeding. Over-activation of an MTP is common and varies between 54% and 84%,[ 12 20 21 ] as specific criteria for massive bleeding in nontrauma patients are lacking.…”

Section: Discussionmentioning

confidence: 99%

Effects of a hospital-wide introduction of a massive transfusion protocol on blood product ratio and blood product waste

Balvers

Coppens

Dieren

et al. 2015

J Emerg Trauma Shock

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Background:Massive transfusion protocols (MTPs) are increasingly used in the transfusion practice and are developed to provide the standardized and early delivery of blood products and procoagulant agents and to supply the transfusion of blood products in a well-balanced ratio.Aim:The aim of this study was to investigate the effect of a hospital-wide introduction of an MTP on blood product ratio and a waste of blood products.Materials and Methods:Retrospective analysis was performed to compare the transfusion practice in massive bleeding patients before and after the introduction of an MTP and between the use of an MTP and transfusion off-protocol. Massive bleeding was defined as an administration of ≥5 units of red blood cells (RBCs) within 12 h.Results:Of 547 massively transfused patients, 192 patients were included in the pre-MTP period and 355 patients in the MTP period. The ratio of RBC to fresh frozen plasma (FFP) and the platelets transfused shifted significantly toward 1:1:1 in the MTP period (P = 0.012). This was mainly caused by a shift in RBC: FFP ratio (P = 0.014). An increase in the waste of blood products was observed, most notably FFPs (P = 0.026). Extending the storage time after thawing reduced the waste of FFPs from 11% to 4%.Conclusion:Hospital-wide introduction of an MTP is an adequate way to achieve a well-balanced transfusion ratio of 1:1:1. This comes at the cost of an increase in the waste of FFPs, which is lowered after extending the duration of storage time after thawing.

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“…We did not evaluate the stability of the coagulation factors in thawed plasma, a product commonly used in clinical medicine in the United States. While this would provide additional information on the in vitro profile for thawed plasma, there is no evidence to suggest that any changes in thawed PF24RT24WB stored over 5 days would be different from those of FFP . European definitions do not distinguish between frozen plasma thawed held at 1 to 6°C less than 24 hours and that held at 1 to 6°C up to 5 days.…”

Section: Discussionmentioning

confidence: 99%

“…While this would provide additional information on the in vitro profile for thawed plasma, there is no evidence to suggest that any changes in thawed PF24RT24WB stored over 5 days would be different from those of FFP. [16][17][18][19][20] European definitions do not distinguish between frozen plasma thawed held at 1 to 6°C less than 24 hours and that held at 1 to 6°C up to 5 days. Likewise, European definitions do not distinguish between plasma frozen within 8 hours of collection and that prepared from room temperature held WB and frozen within 24 hours of collection.…”

Section: Discussionmentioning

confidence: 99%

The bioequivalence of frozen plasma prepared from whole blood held overnight at room temperature compared to fresh‐frozen plasma prepared within eight hours of collection

et al. 2014

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BACKGROUND:Overnight, room temperature hold of whole blood (WB) before leukoreduction and component processing offers significant logistic and cost advantages over WB processed within 8 hours. Plasma prepared from WB held at room temperature overnight (PF24RT24WB) may result in a degradation of plasma coagulation protein activities compared to plasma frozen within 8 hours of collection. In this study, we intended to evaluate the bioequivalence (BE) of PF24RT24WB prepared using a new WB collection, leukoreduction, and storage system compared to freshfrozen plasma (FFP) after 12 months of frozen storage. STUDY DESIGN AND METHODS:We conducted a three-center, three-arm evaluation of the LEUKOSEP HWB-600-XL test system (Hemerus Medical LLC) compared to the RZ2000 control (Fenwal, Inc.). FFP was prepared from WB held at room temperature more than 6 hours and placed at less than −18°C by 8 hours for control (n = 60) and test (n = 60) arms. PF24RT24WB (n = 60) was prepared with the test system from WB held at room temperature and then filtered and processed 20 to 24 hours postcollection. Frozen plasma was tested at 3, 6, and 12 months using a comprehensive panel of protein and coagulation factor assays. RESULTS: The test FFP was BE for all coagulation factors and tested proteins at 12 months. As expected, PF24RT24WB had a reduced Factor (F)VIII activity compared to control FFP (87.1%; 90% confidence interval, 79.4%-93.3%) with the lower confidence limit less than 80%. All other factors were within the BE region. CONCLUSION: Leukoreduced FFP and PF24RT24WB prepared using the LEUKOSEP HWB-600-XL system has been shown to be BE to control leukoreduced FFP with an expected decrease in FVIII activity after overnight hold.

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Coagulation factors and inhibitors in thawed plasma stored at 1–6°C for 5days in China

Cited by 10 publications

References 34 publications

TMT‐Based Quantitative Proteomic Analysis Reveals Proteomic Changes Involved in Longevity

TMT‐Based Quantitative Proteomic Analysis Reveals Proteomic Changes Involved in Longevity

Effects of a hospital-wide introduction of a massive transfusion protocol on blood product ratio and blood product waste

The bioequivalence of frozen plasma prepared from whole blood held overnight at room temperature compared to fresh‐frozen plasma prepared within eight hours of collection

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