Coagulase gene polymorphism of Staphylococcus aureus isolates from dairy cattle in different geographical areas

Abstract

The objectives of this study were to investigate the coagulase gene polymorphism of Staphylococcus aureus isolates obtained from bovine mastitic milk and to determine the resistance of predominant and rare coagulase genotypes to bovine blood neutrophil bactericidal activities. A total of 453 isolates were collected from four countries: the Czech Republic, France, Korea and the United States. The isolates were subtyped into 40 types by restriction fragment length polymorphism (RFLP) of the coagulase gene. Twenty-three strains from predominant and rare genotypes were evaluated for their ability to resist neutrophil bactericidal activities. There were significant (P < 0.01) differences in the average percent neutrophil killing of the predominant (16.7%) and rare (39.7%) genotypes when bacteria were opsonized with antiserum. The results indicate that the profiles of coagulase genotype differ among geographic locations, and only a few genotypes prevail in each location. In addition, the predominant genotypes were more resistant to neutrophil bactericidal activities than rare genotypes.

Molecular Analysis of Coagulase Gene Polymorphism in Clinical Isolates of Methicilin Resistant Staphylococcus aureus by Restriction Fragment Length Polymorphism Based Genotyping

“…We have also evaluated the efficiency of these typing methods in discriminating unrelated S. aureus strains using two restriction enzymes AluI and HaeIII for their typing ability. [19,20] .…”

Subklinik Mastitli İneklerden İzole Edilen Staphylococcus aureus İzolatlarinin Antibiyotik Duyarlılık Profillerinin Çıkarılması ve Koagulaz Geni Polimorfizmine Göre Tiplendirilmesi

“…Raimundo et al [23] examined coa gene type of 151 samples of cattle S. aureus isolates from 76 farms I II III IV I II III IV I II III IV I II III IV P 6 33 by using Coag2 and Coag3 primers and found 6 types of PCR. Su et al [3] investigated coa gene diversity in S. aureus isolates from 4 countries. They reported 5 genotypes were dominant for each country.…”

“…Regional evaluation of antibiotic susceptibilities of S. aureus may help veterinary surgeons [1,2] . Although most of the current S. aureus isolates have different genotypic and phenotypic characteristics, few are known about geographical distribution of those isolates and types of the pathogens in the herd [3] . Previously, distinct classification methods such as phage typing had been applied to both human and cattle originated S. aureus isolates [4,5] .…”

Coagulase Gene Polymorphism in Staphylococcus aureus isolated From Chicken and Pork Marketed in Retail Outlets of Chennai

“…Several researchers have reported polymorphism in coa gene of S. aureus isolated from bovine mastitis and have appreciated the use coa typing as simple and accurate method (Su et al, 1999 andTenover et al, 1994). Van Belkum et al (1998) opined that the discriminatory power of coa gene in typing of S. aureus is due to heterogeneity of the region containing the 81 bp tandem repeats at the 3' coding region of the coagulase gene.…”