Coagulansin-A has beneficial effects on the development of bovine embryos <i>in vitro</i> via HSP70 induction

Khan, Imran; Lee, Kyeong-Lim; Fakruzzaman, Md.; Song, Seok-Hwan; ul-Haq, Ihsan; Mirza, Bushra; Yan, Chunri; Kong, Il‐Keun

doi:10.1042/bsr20150222

Cited by 15 publications

(14 citation statements)

References 47 publications

(50 reference statements)

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“…The numbers of apoptotic and total cells are key factors for embryo quality; the higher the number of cells in an implanted embryo, the higher the chance of live offspring being delivered (van Soom et al 2007). In the present study, coa-A treatment in IVM media at a heat stress of 40.5˚C for 20 h improved blastocyst quality by increasing the total number of cells and decreasing the apoptotic index compared with the control group, which is in agreement with our previous findings at a normal culture temperature (Khan et al 2016).…”

Section: Discussionsupporting

confidence: 93%

“…The goal of this study was to determine the protective effect of coa-A against heat stress in IVM media for blastocyst development. For this purpose, the optimal concentration of coa-A (5 μM) was selected based on our previous data (Khan et al 2016). Oocytes treated with 5 μM coa-A were compared with the untreated control group.…”

Section: Experimental Designmentioning

confidence: 99%

“…Withanolides also have a dynamic anticancer role, operating as anticancer agents in cancer cell lines by enhancing apoptosis, and are also used to treat Alzheimer's disease, Parkinson's disease and dementia (Wang et al 2012). Coagulansin-A (coa-A) is a withanolide derivative, and we reported for the first time that it improves bovine embryo development in vitro in normal culture conditions (Khan et al 2016). Various environmental factors affect the fertility of dairy animals.…”

Section: Introductionmentioning

confidence: 99%

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Improvement of in vitro-produced bovine embryo treated with coagulansin-A under heat-stressed condition

Khan

Lee

et al. 2017

Reproduction

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Heat stress has large effects on reproduction including conception rate in cattle. In this study, we examined the effects of coagulansin-A (coa-A), a steroidal lactone, on acquired thermo tolerance during in vitro production of bovine embryos. Oocytes were incubated in in vitro maturation (IVM) media with or without coa-A at two different temperatures, 40.5˚C and 42˚C, for 20 h. The treatment of coa-A significantly improved blastocyst development only at 40.5˚C (P < 0.05). Interestingly, immunofluorescence analysis demonstrated that coa-A induced heat shock protein 70 (HSP70) and phosphatidylinositol-3-kinase (PI3K), but significantly attenuated nuclear factor kappa B (NF-κB) and cyclooxygenase-2 (COX2). To determine the expression patterns of related genes at the transcription level, qRT-PCR was performed. Expression of HSP70 and PI3K was elevated, whereas expression of NF-κB, COX2 and inducible nitric oxide synthase (iNOS) was significantly (P < 0.05) downregulated in the coa-A-treated group compared with the control group. Moreover, pro-apoptotic genes were downregulated, and antiapoptic genes were upregulated in the coa-A group. We also counted the total cell number and apoptotic nuclei at the blastocyst and found that more cell numbers (143.1 ± 1.5) and less apoptotic damages (6.4 ± 0.5) in the coa-A treatment group comparing to control group (131.4 ± 2.0 and 10.8 ± 0.5), indicating the enhanced embryo quality. In conclusion, our results demonstrate that the coa-A not only improved the blastocyst development in vitro but also increased their resistance to heat stress condition through induction of HSP70/PI3K.

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Section: Discussionsupporting

confidence: 93%

Section: Experimental Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Improvement of in vitro-produced bovine embryo treated with coagulansin-A under heat-stressed condition

Khan

Lee

et al. 2017

Reproduction

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“…This result is consistent with a recently published study that addition of coagulansin‐A, a ROS‐scavenging compound, to IVM media induced HSP70 expression in bovine embryos, and this protein after inducing in the culture system improved the embryo quality and efficiency (Khan et al . ).…”

Section: Discussionmentioning

confidence: 97%

Protective effects of melatonin on the in vitro developmental competence of bovine oocytes

Pang

Zhao

Sun

et al. 2017

Animal Science Journal

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The present study investigated the effects of melatonin on bovine oocyte maturation and subsequent embryonic development in vitro. Results showed that the nuclear and cytoplasmic maturation, characterized by first polar body extrusion, normal distribution of cortical granules and mitochondria, as well as increased mitochondrial membrane potential, were significantly improved in 10 mol/L melatonin-treated oocytes. Melatonin supplementation reduced intracellular reactive oxygen species level and enhanced glutathione production. Meanwhile, the presence of melatonin (10 mol/L) during oocyte maturation resulted in a decreased early apoptotic rate in oocytes. After in vitro fertilization, oocytes receiving melatonin supplementation exhibited a significantly higher blastocyst formation rate and yielded a markedly lower number of apoptotic cells. Mechanistic explorations showed that addition of 10 mol/L melatonin to in vitro maturation media significantly attenuated the transcript level of caspase-3, while the expressions of BCL-2, XIAP, CAT and HSP70 were significantly reinforced in the resultant embryos. Taken together, melatonin ameliorates bovine oocyte maturation potential, and the beneficial effects can affect subsequent embryonic development. The protective role of melatonin may be due to its anti-apoptotic and anti-oxidative activities.

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“… and embryos; Khan et al . ), but these studies did not discriminate between HSPA1A and HSPA1B expression. In fact, based on the sequence of the primers that were used, in some cases they could have potentially targeted both genes.…”

Section: Discussionmentioning

confidence: 77%

Characterization of a deletion in the Hsp70 cluster in the bovine reference genome

et al. 2017

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The 70 kilodalton heat shock proteins (Hsp70) are highly conserved molecular chaperones which have a crucial role in the stress response of the cell. In mammals, the Hsp70 proteins are encoded by a cluster of three genes: HSPA1A, HSPA1B and HSPA1L. In bovines, this cluster is located on chromosome 23 downstream of the major histocompatibility complex (BoLA). We detected inconsistencies in the location of markers on the Hsp70 genes reported in the literature that pointed to a potential deletion in the bovine reference genome UMD 3.1.1. An in silico analysis of the bovine genomic region of the Hsp70 cluster, using available information from public databases, confirmed the existence of a deletion of 11.1-kb spanning the HSPA1B gene and the intergenic region between HSPA1B and HSPA1A. Although we originally considered this an assembly error, it is most likely a particular condition of L1 Dominette 01449, the cow sequenced in the Bovine Genome Project. Moreover, we suggest a new classification of bovine Hsp70 sequences reported in NCBI and a reassignment of the location of SNPs from dbSNP that map to the deletion on BTA23. We also compared the location of selected transcription factor binding sites on the promoters of HSPA1A and HSPA1B. The results generated in the present work could be helpful to refine the reference genome of an important livestock species and also to understand the role and the regulation of the bovine Hsp70 genes.

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Coagulansin-A has beneficial effects on the development of bovine embryos in vitro via HSP70 induction

Cited by 15 publications

References 47 publications

Improvement of in vitro-produced bovine embryo treated with coagulansin-A under heat-stressed condition

Improvement of in vitro-produced bovine embryo treated with coagulansin-A under heat-stressed condition

Protective effects of melatonin on the in vitro developmental competence of bovine oocytes

Characterization of a deletion in the Hsp70 cluster in the bovine reference genome

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