Coaggregation and biofilm growth of Granulicatella spp. with Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans

Karched, Maribasappa; Bhardwaj, Radhika G.; Asikainen, Sirkka

doi:10.1186/s12866-015-0439-z

Cited by 57 publications

(49 citation statements)

References 62 publications

(81 reference statements)

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“…Biofilm formation by Granulicatella spp. was reported by Karched et al [23]. In agreement with the results obtained in this study, using plastic plate adherent assay with crystal violet stain, it was revealed that G. adiacens possesses the ability to form biofilms.…”

Section: Discussionsupporting

confidence: 93%

“…Then, 200 µL of ethanol (95%) was added to each well, and the plate was incubated at room temperature for 10 min. Then, 100 µL sample from each well were used to measure the optical densities at 590 nm using a microplate reader (Molecular Devices LLC, Tokyo, Japan) according to the method described by Karched et al [23]. For performing scanning electron microscopy, G. adiacens and A. defectiva strains were grown in THB at 37°C for 24 h. After washing with PBS, the cells were fixed in an equal volume of 2.5% glutaraldehyde in PBS for 30 min.…”

Section: Production Of Eps Biofilmmentioning

confidence: 99%

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Contribution of different adherent properties of Granulicatella adiacens and Abiotrophia defectiva to their associations with oral colonization and the risk of infective endocarditis

et al. 2020

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Granulicatella adiacens (G. adiacens) and Abiotrophia defectiva (A. defectiva) colonize the oral cavity and form part of the normal flora in the intestinal and genitourinary tracts. As reported previously, the frequency of isolation of G. adiacens from the oral cavity was much higher than that of A. defectiva. However, it has been reported that compared with G. adiacens, A. defectiva was isolated at considerably higher frequencies from the blood of patients with infective endocarditis (IE). Hence, in this study, the in vitro interaction of G. adiacens and A. defectiva strains with host surfaces and biofilm formation was examined to assess whether their different adhesive properties contribute to their associations with oral colonization and IE, respectively. G. adiacens exhibited an increased binding ability to saliva-coated hydroxyapatite beads than A. defectiva following the addition of CaCl 2. Furthermore, biofilm formation was observed only for G. adiacens with the use of a polystyrene tube and scanning electron microscopy analysis. Conversely, A. defectiva displayed significantly greater adherence to human umbilical vein endothelial cells and immobilized fibronectin than G. adiacens. These findings suggest that differences in binding properties to host components imply specific binding mechanisms in G. adiacwens and A. defectiva, which might mediate selective colonization in the oral cavity or are associated with the pathogenicity of endocarditis.

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Section: Discussionsupporting

confidence: 93%

Section: Production Of Eps Biofilmmentioning

confidence: 99%

Contribution of different adherent properties of Granulicatella adiacens and Abiotrophia defectiva to their associations with oral colonization and the risk of infective endocarditis

et al. 2020

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“…Of note, coaggregation assays were carried out using planktonic cultures and aimed to assess the possible mechanism behind the development of bacterial biofilms (Rickard et al, 2003). However, it has been reported that there is not always a direct relation between coaggregation and biofilm formation (Karched et al, 2015). In brief, 500 µL of G. vaginalis (10 7 CFU/mL) was combined with 500 µL of A. vaginae (10 7 CFU/mL) in 24-well plates (Thermo Fisher Scientific) and incubated for 4 h, at 37 • C, under anaerobic conditions.…”

Section: Coaggregation Assaysmentioning

confidence: 99%

Gardnerella vaginalis Enhances Atopobium vaginae Viability in an in vitro Model

Castro¹,

Roșca²,

Cools

et al. 2020

Front. Cell. Infect. Microbiol.

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Bacterial vaginosis (BV) is the most common vaginal infection among women of reproductive age. A hallmark of BV is the presence of a highly structured polymicrobial biofilm on the vaginal epithelium, presumably initiated by facultative anaerobes of the genus Gardnerella, which then becomes a scaffold for other species to adhere to. One of the species often found incorporated in Gardnerella mediated biofilms is Atopobium vaginae. Interestingly, A. vaginae is very rarely found without the presence of Gardnerella. However, not much is known regarding the interactions between A. vaginae and Gardnerella species. This study assessed biological interactions between Gardnerella vaginalis and A. vaginae. In our in vitro model, by using specific Gardnerella and A. vaginae Peptide Nucleic Acid (PNA)-Fluorescence In Situ Hybridization (FISH) probes, we confirmed that A. vaginae was able to incorporate a pre-formed G. vaginalis biofilm, accounting for up to 20% of the total number of biofilm cells. However, our findings showed that almost 92% of A. vaginae cells lost viability after 48 h of mono-species planktonic growth, but were able to maintain viability when co-cultured with Gardnerella or after pre-conditioning with cell-free supernatant of Gardnerella cultures. While the in vitro conditions are very different from the in vivo microenvironment, this study contributes to a better understanding of why A. vaginae vaginal colonization rarely occurs in the absence of Gardnerella. Overall, this highlights the importance of microbial interactions between BV-associated bacteria and demands more studies focused on the polymicrobial bacterial communities found in BV.

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“…The congregation of Granulicatella spp. with Aggregatibacter actinomycetemcomitans [48] has been positively correlated to periodontitis [49], as well as serious infections outside of the oral cavity, such as infective endocarditis [50]. Veillonella is a genus of Gram-negative anaerobic bacteria mainly found in the oral and gastrointestinal tracts.…”

Section: Discussionmentioning

confidence: 99%

Shifts in the bacterial community of supragingival plaque related to metabolic associated fatty liver disease

Zhao

Yuan

Wang

et al. 2020

Preprint

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Background Metabolic-associated fatty liver disease (MAFLD), also known as the hepatic manifestation of metabolic disorders, has become one of the most common chronic liver diseases worldwide. The associations between some oral resident microbes and MAFLD have been described. However, changes to the oral microbial community in patients with MAFLD remain unknown. Methods In this study, variations to the supragingival microbiota of MAFLD patients were identified. The microbial genetic profile of supragingival plaque samples from 24 MAFLD patients and 22 healthy participants were analyzed by 16S rDNA sequencing and bioinformatics analysis. Clinical variables, including indicators of insulin resistance, obesity, blood lipids, and hepatocellular damage, were evaluated with laboratory tests and physical examinations. Results The results showed that the diversity of the supragingival microbiota in MAFLD patients was significantly higher than that in healthy individuals. Weighted UniFrac principal coordinates analysis and partial least squares discriminant analysis showed that the samples from the MAFLD and control groups formed separate clusters (Adonis, P = 0.0120). There were 27 taxa with differential distributions (linear discriminant analysis, LDA༞2.0) between two groups, among which Actinomyces and Prevotella 2 spp. were over-represented in the MAFLD group with highest LDA score, while Neisseria and Bergeyella spp. were more abundant in the control group. Co-occurrence networks of the top 50 abundant genera in the two groups suggested that the inter-genera relationships were also altered in the supragingival plaque of MAFLD patients. In addition, as risk factors for the development of MAFLD, insulin resistance was positively correlated with the abundances of Granulicatella, Veillonella, Streptococcus, and Scardovia spp., while obesity was positively correlated to the abundances of Streptococcus, Oslenella, Scardovia, and Selenomonas spp. Metagenomic predictions based on Phylogenetic Investigation of Communities by Reconstruction of Unobserved States revealed that pathways related to sugar (mainly free sugar) metabolism were enriched in the supragingival plaque of the MAFLD group. Conclusions In conclusion, as compared to healthy individuals, component and interactional dysbioses were observed in the supragingival microbiota of the MAFLD group, which could be further explored as potential oral biomarkers of the MAFLD.

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Coaggregation and biofilm growth of Granulicatella spp. with Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans

Cited by 57 publications

References 62 publications

Contribution of different adherent properties of <i>Granulicatella adiacens</i> and <i>Abiotrophia defectiva</i> to their associations with oral colonization and the risk of infective endocarditis

Contribution of different adherent properties of <i>Granulicatella adiacens</i> and <i>Abiotrophia defectiva</i> to their associations with oral colonization and the risk of infective endocarditis

Gardnerella vaginalis Enhances Atopobium vaginae Viability in an in vitro Model

Shifts in the bacterial community of supragingival plaque related to metabolic associated fatty liver disease

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