Coadsorption of HIV-1 p24 and gp120 proteins to surfactant-free anionic PLA nanoparticles preserves antigenicity and immunogenicity

Lamalle-Bernard, Delphine; Munier, Sevérine; Compagnon, Christelle; Charles, Marie‐Hélène; Kalyanaraman, Vaniambadi S.; Delair, Thierry; Verrier, Bernard; Ataman-Önal, Yasemin

doi:10.1016/j.jconrel.2006.07.006

Cited by 73 publications

(45 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the case of growth factors in culture medium, it has been widely observed that the behavior of colloidal carriers is altered with the adhesion of free bioactive molecules. The adsorption of human immunodeficiency virus-1 p24 and gp120 proteins from a solution controlled the release of vaccine from polylactide [6]. Adherent Tween 80 induced a specific brain-targeting effect of polylactide NPs [7,8].…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis of bone marrow stromal cells with uptake of heparin‐functionalized poly(lactide‐co‐glycolide) nanoparticles during differentiation towards neurons

2010

View full text Add to dashboard Cite

This study analyzes the varying electrophoretic mobility and zeta potential of bone marrow stromal cells (BMSCs) during their differentiation towards neurons. Electrophoresis of primary BMSCs and neuron growth factor (NGF)-induced neuron-like cells with the uptake of heparin-functionalized poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) are also investigated. Immunofluorescent images revealed that a high concentration of NGF accelerated the differentiation of BMSCs into neurons. When the concentration of NGF increased, the absolute values of electrophoretic mobility and zeta potential of the differentiating BMSCs increased. In addition, a longer inductive period yielded higher charge of the differentiating BMSCs and a smaller uptake amount of heparin-functionalized PLGA NPs. However, an increase in the loading efficiency of heparin on PLGA NPs enhanced the uptake and reduced the electrical characteristics of the primary and differentiating BMSCs. Hence, a general rule is drawn that an increase in the uptake of heparin-functionalized PLGA NPs decreased the electrophoretic mobility and zeta potential of BMSCs during differentiation towards neurons.

show abstract

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis of bone marrow stromal cells with uptake of heparin‐functionalized poly(lactide‐co‐glycolide) nanoparticles during differentiation towards neurons

2010

View full text Add to dashboard Cite

show abstract

“…Attempts at subunit vaccination with Env antigen are well documented [10,[34][35][36]. In particular, recent studies have elegantly employed fusion proteins that spontaneously self-assemble into nanoparticles mimicking virus-like particles for multivalent display of Env-based immunogens, including germline targeting gp120 outer domains, Clade C ZM109-based V1V2 domain trimers, BG505 gp120 trimers, and BG505.…”

Section: Self-assembling 'Virus-like' Nanoparticles For Presentation mentioning

confidence: 99%

Vaccine Nanoparticles for Protection Against HIV Infection

Aikins

Bazzill

Moon

2017

Nanomedicine (Lond.)

View full text Add to dashboard Cite

The development of a successful vaccine against HIV is a major global challenge. Antiretroviral therapy is the standard treatment against HIV-1 infection. However, only 46% of the eligible people received the therapy in 2015. Furthermore, suboptimal adherence poses additional obstacles. Therefore, there is an urgent need for an HIV-1 vaccine. The most promising clinical trial to date is Phase III RV144, which for the first time demonstrated the feasibility of vaccine-mediated immune protection against HIV-1. Nevertheless, its 31% efficacy and limited durability underscore major hurdles. Here, we discuss recent progress in HIV-1 vaccine development with a special emphasis on nanovaccines, which are at the forefront of efforts to develop a successful HIV-1 vaccine.

show abstract

“…Moreover, the exposure of proteins to organic solvents, required to dissolve the polymer, makes them highly susceptible to denaturation leading to the loss of antigenic epitope recognition [208]. This can partially be overcome by adsorption of proteins on the particle surface [209,210]. Additionally, a rather expensive up-scaling process and clean-up procedure to ensure sterile production constitute difficult obstacles [211].…”

Section: Polymers Based On Lactic Acid (Pla) and Glycolic Acid (Plga)mentioning

confidence: 99%

“Wrapped Up” Vaccines in the Context of HIV-1 Immunotherapy

et al. 2012

View full text Add to dashboard Cite

show abstract

Coadsorption of HIV-1 p24 and gp120 proteins to surfactant-free anionic PLA nanoparticles preserves antigenicity and immunogenicity

Cited by 73 publications

References 29 publications

Capillary electrophoresis of bone marrow stromal cells with uptake of heparin‐functionalized poly(lactide‐co‐glycolide) nanoparticles during differentiation towards neurons

Capillary electrophoresis of bone marrow stromal cells with uptake of heparin‐functionalized poly(lactide‐co‐glycolide) nanoparticles during differentiation towards neurons

Vaccine Nanoparticles for Protection Against HIV Infection

“Wrapped Up” Vaccines in the Context of HIV-1 Immunotherapy

Contact Info

Product

Resources

About